Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8
Abstract Cupriavidus sp. strain CNP-8 isolated from a pesticide-contaminated soil was able to utilize 2-chloro-4-nitrophenol (2C4NP) as a sole source of carbon, nitrogen and energy, together with the release of nitrite and chloride ions. It could degrade 2C4NP at temperatures from 20 to 40 °C and at...
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| Format: | Article |
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SpringerOpen
2018-03-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-018-0574-7 |
| _version_ | 1828471267556065280 |
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| author | Jun Min Jinpei Wang Weiwei Chen Xiaoke Hu |
| author_facet | Jun Min Jinpei Wang Weiwei Chen Xiaoke Hu |
| author_sort | Jun Min |
| collection | DOAJ |
| description | Abstract Cupriavidus sp. strain CNP-8 isolated from a pesticide-contaminated soil was able to utilize 2-chloro-4-nitrophenol (2C4NP) as a sole source of carbon, nitrogen and energy, together with the release of nitrite and chloride ions. It could degrade 2C4NP at temperatures from 20 to 40 °C and at pH values from 5 to 10, and degrade 2C4NP as high as 1.6 mM. Kinetics assay showed that biodegradation of 2C4NP followed Haldane substrate inhibition model, with the maximum specific growth rate (μ max) of 0.148/h, half saturation constant (K s) of 0.022 mM and substrate inhibition constant (K i) of 0.72 mM. Strain CNP-8 was proposed to degrade 2C4NP with hydroxyquinol (1,2,4-benzenetriol, BT) as the ring-cleavage substrate. The 2C4NP catabolic pathway in strain CNP-8 is significant from those reported in other Gram-negative 2C4NP utilizers. Enzymatic assay indicated that the monooxygenase initiating 2C4NP catabolism had different substrates specificity compared with previously reported 2C4NP monooxygenations. Capillary assays showed that strain CNP-8 exhibited metabolism-dependent chemotactic response toward 2C4NP at the optimum concentration of 0.5 mM with a maximum chemotaxis index of 37.5. Furthermore, microcosm studies demonstrated that strain CNP-8, especially the pre-induced cells, could remove 2C4NP rapidly from the 2C4NP-contaminated soil. Considering its adaptability to pH and temperature fluctuations and great degradation efficiency against 2C4NP, strain CNP-8 could be a promising candidate for the bioremediation of 2C4NP-contaminated sites. |
| first_indexed | 2024-12-11T05:09:29Z |
| format | Article |
| id | doaj.art-2c4a4b7ba4444a2c9a1aaa43b74385e9 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-11T05:09:29Z |
| publishDate | 2018-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-2c4a4b7ba4444a2c9a1aaa43b74385e92022-12-22T01:19:58ZengSpringerOpenAMB Express2191-08552018-03-018111110.1186/s13568-018-0574-7Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8Jun Min0Jinpei Wang1Weiwei Chen2Xiaoke Hu3Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of SciencesKey Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of SciencesKey Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of SciencesKey Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of SciencesAbstract Cupriavidus sp. strain CNP-8 isolated from a pesticide-contaminated soil was able to utilize 2-chloro-4-nitrophenol (2C4NP) as a sole source of carbon, nitrogen and energy, together with the release of nitrite and chloride ions. It could degrade 2C4NP at temperatures from 20 to 40 °C and at pH values from 5 to 10, and degrade 2C4NP as high as 1.6 mM. Kinetics assay showed that biodegradation of 2C4NP followed Haldane substrate inhibition model, with the maximum specific growth rate (μ max) of 0.148/h, half saturation constant (K s) of 0.022 mM and substrate inhibition constant (K i) of 0.72 mM. Strain CNP-8 was proposed to degrade 2C4NP with hydroxyquinol (1,2,4-benzenetriol, BT) as the ring-cleavage substrate. The 2C4NP catabolic pathway in strain CNP-8 is significant from those reported in other Gram-negative 2C4NP utilizers. Enzymatic assay indicated that the monooxygenase initiating 2C4NP catabolism had different substrates specificity compared with previously reported 2C4NP monooxygenations. Capillary assays showed that strain CNP-8 exhibited metabolism-dependent chemotactic response toward 2C4NP at the optimum concentration of 0.5 mM with a maximum chemotaxis index of 37.5. Furthermore, microcosm studies demonstrated that strain CNP-8, especially the pre-induced cells, could remove 2C4NP rapidly from the 2C4NP-contaminated soil. Considering its adaptability to pH and temperature fluctuations and great degradation efficiency against 2C4NP, strain CNP-8 could be a promising candidate for the bioremediation of 2C4NP-contaminated sites.http://link.springer.com/article/10.1186/s13568-018-0574-72-Chloro-4-nitrophenolHydroxyquinol pathwayKineticsChemotaxisCupriavidus sp. strain CNP-8 |
| spellingShingle | Jun Min Jinpei Wang Weiwei Chen Xiaoke Hu Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8 AMB Express 2-Chloro-4-nitrophenol Hydroxyquinol pathway Kinetics Chemotaxis Cupriavidus sp. strain CNP-8 |
| title | Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8 |
| title_full | Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8 |
| title_fullStr | Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8 |
| title_full_unstemmed | Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8 |
| title_short | Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8 |
| title_sort | biodegradation of 2 chloro 4 nitrophenol via a hydroxyquinol pathway by a gram negative bacterium cupriavidus sp strain cnp 8 |
| topic | 2-Chloro-4-nitrophenol Hydroxyquinol pathway Kinetics Chemotaxis Cupriavidus sp. strain CNP-8 |
| url | http://link.springer.com/article/10.1186/s13568-018-0574-7 |
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