Differential and interactive effects of cytoplasmic substitution and seed ageing on submergence stress response in wheat (Triticum aestivum L.)
Cytoplasmic genomes affect various phenotypes, including abiotic stress responses, through interaction with nuclear genomes in plants. We focused on the effects of cytoplasmic substitution on germination and seedling growth in combinations with submergence and seed ageing, both of which are known to...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2018.1549960 |
| _version_ | 1818929068384452608 |
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| author | Shotaro Takenaka Ryohei Yamamoto Chiharu Nakamura |
| author_facet | Shotaro Takenaka Ryohei Yamamoto Chiharu Nakamura |
| author_sort | Shotaro Takenaka |
| collection | DOAJ |
| description | Cytoplasmic genomes affect various phenotypes, including abiotic stress responses, through interaction with nuclear genomes in plants. We focused on the effects of cytoplasmic substitution on germination and seedling growth in combinations with submergence and seed ageing, both of which are known to inhibit these traits posing a challenge in agriculture and seed banking. We carried out comparative phenotypic studies of submergence and seed ageing effects using a series of nucleus–cytoplasm (NC) hybrids of wheat, in which 11 heterologous cytoplasms of Triticum and Aegilops species were combined with a common nucleus. Adopting the test-tube bioassay, germination and seedling growth were studied using aged and non-aged seeds. Imbibed seeds were subjected to 3-days submergence followed by incubation under de-submergence conditions. Seed ageing reduced the germination rates in NC hybrids. Submergence and seed ageing both caused reduction of seedling growth evaluated by shoot length in all or most of the lines. The magnitude of shoot growth inhibition by submergence and seed ageing varied greatly among NC hybrids compared with the nuclear donor, and three distinct response types were recognized. Submergence and seed ageing, in combination, caused leaf chlorosis in most of NC hybrids. Our results suggested that the observed diverse effects of cytoplasmic substitution were exerted through differential interactions with submergence and seed ageing. Further studies are needed to clarify the mechanisms underlying cytoplasmic genome diversity and interaction with nuclear genomes affecting submergence and seed ageing responses in wheat. |
| first_indexed | 2024-12-20T03:38:55Z |
| format | Article |
| id | doaj.art-2c4ae7b5bcd5411cbfc8ebb880da99b5 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-20T03:38:55Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-2c4ae7b5bcd5411cbfc8ebb880da99b52022-12-21T19:54:48ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302019-01-01331758510.1080/13102818.2018.15499601549960Differential and interactive effects of cytoplasmic substitution and seed ageing on submergence stress response in wheat (Triticum aestivum L.)Shotaro Takenaka0Ryohei Yamamoto1Chiharu Nakamura2Ryukoku UniversityRyukoku UniversityRyukoku UniversityCytoplasmic genomes affect various phenotypes, including abiotic stress responses, through interaction with nuclear genomes in plants. We focused on the effects of cytoplasmic substitution on germination and seedling growth in combinations with submergence and seed ageing, both of which are known to inhibit these traits posing a challenge in agriculture and seed banking. We carried out comparative phenotypic studies of submergence and seed ageing effects using a series of nucleus–cytoplasm (NC) hybrids of wheat, in which 11 heterologous cytoplasms of Triticum and Aegilops species were combined with a common nucleus. Adopting the test-tube bioassay, germination and seedling growth were studied using aged and non-aged seeds. Imbibed seeds were subjected to 3-days submergence followed by incubation under de-submergence conditions. Seed ageing reduced the germination rates in NC hybrids. Submergence and seed ageing both caused reduction of seedling growth evaluated by shoot length in all or most of the lines. The magnitude of shoot growth inhibition by submergence and seed ageing varied greatly among NC hybrids compared with the nuclear donor, and three distinct response types were recognized. Submergence and seed ageing, in combination, caused leaf chlorosis in most of NC hybrids. Our results suggested that the observed diverse effects of cytoplasmic substitution were exerted through differential interactions with submergence and seed ageing. Further studies are needed to clarify the mechanisms underlying cytoplasmic genome diversity and interaction with nuclear genomes affecting submergence and seed ageing responses in wheat.http://dx.doi.org/10.1080/13102818.2018.1549960aegilopscytoplasm substitutionnucleus–cytoplasm hybridsseed ageingsubmergencewheat (triticum) |
| spellingShingle | Shotaro Takenaka Ryohei Yamamoto Chiharu Nakamura Differential and interactive effects of cytoplasmic substitution and seed ageing on submergence stress response in wheat (Triticum aestivum L.) Biotechnology & Biotechnological Equipment aegilops cytoplasm substitution nucleus–cytoplasm hybrids seed ageing submergence wheat (triticum) |
| title | Differential and interactive effects of cytoplasmic substitution and seed ageing on submergence stress response in wheat (Triticum aestivum L.) |
| title_full | Differential and interactive effects of cytoplasmic substitution and seed ageing on submergence stress response in wheat (Triticum aestivum L.) |
| title_fullStr | Differential and interactive effects of cytoplasmic substitution and seed ageing on submergence stress response in wheat (Triticum aestivum L.) |
| title_full_unstemmed | Differential and interactive effects of cytoplasmic substitution and seed ageing on submergence stress response in wheat (Triticum aestivum L.) |
| title_short | Differential and interactive effects of cytoplasmic substitution and seed ageing on submergence stress response in wheat (Triticum aestivum L.) |
| title_sort | differential and interactive effects of cytoplasmic substitution and seed ageing on submergence stress response in wheat triticum aestivum l |
| topic | aegilops cytoplasm substitution nucleus–cytoplasm hybrids seed ageing submergence wheat (triticum) |
| url | http://dx.doi.org/10.1080/13102818.2018.1549960 |
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