6-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway
This study aimed to investigate how 6-bromoindirubin-3’-oxime (BIO) increases the osteogenic differentiation of canine bone mesenchymal stem cells (BMSCs) and the role of the Wnt/β-catenin signaling pathway in this process. We mimicked the effect of Wnt by adding BIO to the culture medium of BMSCs a...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academia Brasileira de Ciências
2019-03-01
|
| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100610&lng=en&tlng=en |
| _version_ | 1818963057830789120 |
|---|---|
| author | XIAO-E ZHAO ZHENSHAN YANG ZHEN GAO JUNBANG GE QIANG WEI BAOHUA MA |
| author_facet | XIAO-E ZHAO ZHENSHAN YANG ZHEN GAO JUNBANG GE QIANG WEI BAOHUA MA |
| author_sort | XIAO-E ZHAO |
| collection | DOAJ |
| description | This study aimed to investigate how 6-bromoindirubin-3’-oxime (BIO) increases the osteogenic differentiation of canine bone mesenchymal stem cells (BMSCs) and the role of the Wnt/β-catenin signaling pathway in this process. We mimicked the effect of Wnt by adding BIO to the culture medium of BMSCs and examined whether canonical Wnt signaling positively affects the differentiation of these cells into osteoblasts. Canine BMSCs were cultured with 0.5 and 1.0 μM BIO under osteogenic conditions and then differentiation markers were investigated. It was found that BIO significantly increased the activity of alkaline phosphatase (ALP), the number of ALP-positive cells, the mineralization level and calcium deposits. Moreover, cells cultured with 0.5 and 1.0 μM BIO exhibited detectable β-catenin expression in their nuclei, and showed upregulated β-catenin and glycogen synthase kinase 3 beta(GSK3β) phosphorylation compared to untreated cells. In addition, BIO enhanced the mRNA expression of osteoblast differentiation markers such as ALP, runt-related transcription factor 2, collagen I, osteocalcin, and osteonectin. In conclusion, BIO upregulated GSK3β phosphorylation and inhibited its activity, thereby activating the Wnt/β-catenin signaling pathway and promoting the osteogenic differentiation of canine BMSCs. The effect of 1.0 μM BIO on BMSCs differentiation was stronger than that of 0.5 μM BIO. |
| first_indexed | 2024-12-20T12:39:10Z |
| format | Article |
| id | doaj.art-2c4d8a9d75b941018ec25883056bf752 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-20T12:39:10Z |
| publishDate | 2019-03-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-2c4d8a9d75b941018ec25883056bf7522022-12-21T19:40:32ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902019-03-0191110.1590/0001-3765201920180459S0001-376520190001006106-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathwayXIAO-E ZHAOZHENSHAN YANGZHEN GAOJUNBANG GEQIANG WEIBAOHUA MAThis study aimed to investigate how 6-bromoindirubin-3’-oxime (BIO) increases the osteogenic differentiation of canine bone mesenchymal stem cells (BMSCs) and the role of the Wnt/β-catenin signaling pathway in this process. We mimicked the effect of Wnt by adding BIO to the culture medium of BMSCs and examined whether canonical Wnt signaling positively affects the differentiation of these cells into osteoblasts. Canine BMSCs were cultured with 0.5 and 1.0 μM BIO under osteogenic conditions and then differentiation markers were investigated. It was found that BIO significantly increased the activity of alkaline phosphatase (ALP), the number of ALP-positive cells, the mineralization level and calcium deposits. Moreover, cells cultured with 0.5 and 1.0 μM BIO exhibited detectable β-catenin expression in their nuclei, and showed upregulated β-catenin and glycogen synthase kinase 3 beta(GSK3β) phosphorylation compared to untreated cells. In addition, BIO enhanced the mRNA expression of osteoblast differentiation markers such as ALP, runt-related transcription factor 2, collagen I, osteocalcin, and osteonectin. In conclusion, BIO upregulated GSK3β phosphorylation and inhibited its activity, thereby activating the Wnt/β-catenin signaling pathway and promoting the osteogenic differentiation of canine BMSCs. The effect of 1.0 μM BIO on BMSCs differentiation was stronger than that of 0.5 μM BIO.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100610&lng=en&tlng=enBone mesenchymal stem cellsbromoindirubin oximeosteogenetic differentiationcanine |
| spellingShingle | XIAO-E ZHAO ZHENSHAN YANG ZHEN GAO JUNBANG GE QIANG WEI BAOHUA MA 6-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway Anais da Academia Brasileira de Ciências Bone mesenchymal stem cells bromoindirubin oxime osteogenetic differentiation canine |
| title | 6-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway |
| title_full | 6-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway |
| title_fullStr | 6-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway |
| title_full_unstemmed | 6-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway |
| title_short | 6-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway |
| title_sort | 6 bromoindirubin 3 oxime promotes osteogenic differentiation of canine bmscs through inhibition of gsk3β activity and activation of the wnt β catenin signaling pathway |
| topic | Bone mesenchymal stem cells bromoindirubin oxime osteogenetic differentiation canine |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100610&lng=en&tlng=en |
| work_keys_str_mv | AT xiaoezhao 6bromoindirubin3oximepromotesosteogenicdifferentiationofcaninebmscsthroughinhibitionofgsk3bactivityandactivationofthewntbcateninsignalingpathway AT zhenshanyang 6bromoindirubin3oximepromotesosteogenicdifferentiationofcaninebmscsthroughinhibitionofgsk3bactivityandactivationofthewntbcateninsignalingpathway AT zhengao 6bromoindirubin3oximepromotesosteogenicdifferentiationofcaninebmscsthroughinhibitionofgsk3bactivityandactivationofthewntbcateninsignalingpathway AT junbangge 6bromoindirubin3oximepromotesosteogenicdifferentiationofcaninebmscsthroughinhibitionofgsk3bactivityandactivationofthewntbcateninsignalingpathway AT qiangwei 6bromoindirubin3oximepromotesosteogenicdifferentiationofcaninebmscsthroughinhibitionofgsk3bactivityandactivationofthewntbcateninsignalingpathway AT baohuama 6bromoindirubin3oximepromotesosteogenicdifferentiationofcaninebmscsthroughinhibitionofgsk3bactivityandactivationofthewntbcateninsignalingpathway |