On-Site Multisample Determination of Chlorogenic Acid in Green Coffee by Chemiluminiscent Imaging
The potential of antioxidants in preventing several diseases has attracted great attention in recent years. Indeed, these products are part of a multi-billion industry. However, there is a lack of scientific information about safety, quality, doses, and changes over time. In the present work, a simp...
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MDPI AG
2023-02-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/6/1/20 |
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| author | Sergi Mallorca-Cebria Yolanda Moliner-Martinez Carmen Molins-Legua Pilar Campins-Falcó |
| author_facet | Sergi Mallorca-Cebria Yolanda Moliner-Martinez Carmen Molins-Legua Pilar Campins-Falcó |
| author_sort | Sergi Mallorca-Cebria |
| collection | DOAJ |
| description | The potential of antioxidants in preventing several diseases has attracted great attention in recent years. Indeed, these products are part of a multi-billion industry. However, there is a lack of scientific information about safety, quality, doses, and changes over time. In the present work, a simple multisample methodology based on chemiluminiscent imaging to determine chlorogenic acid (CHLA) in green coffee samples has been proposed. The multi-chemiluminiscent response was obtained after a luminol-persulfate reaction at pH 10.8 in a multiplate followed by image capture with a charge-coupled device (CCD) camera as a readout system. The chemiluminiscent image was used as an analytical response by measuring the luminescent intensity at 0 °C with the CCD camera. Under the optimal conditions, the detection limit was 20 µM and precision was also adequate with RSD < 12%. The accuracy of the proposed system was evaluated by studying the matrix effect, using a standard addition method. Recoveries of chlorogenic acid ranged from 93–94%. The use of the CCD camera demonstrated advantages such as analysis by image inspection, portability, and easy-handling which is of particular relevance in the application for quality control in industries. Furthermore, multisample analysis was allowed by one single image saving time, energy, and cost. The proposed methodology is a promising sustainable analytical tool for quality control to ensure green coffee safety through dosage control and proper labelling preventing potential frauds. |
| first_indexed | 2024-03-11T08:20:34Z |
| format | Article |
| id | doaj.art-2c53602ec8fe41b8a8ef7e6b9b2ec771 |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-03-11T08:20:34Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-2c53602ec8fe41b8a8ef7e6b9b2ec7712023-11-16T22:26:26ZengMDPI AGMethods and Protocols2409-92792023-02-01612010.3390/mps6010020On-Site Multisample Determination of Chlorogenic Acid in Green Coffee by Chemiluminiscent ImagingSergi Mallorca-Cebria0Yolanda Moliner-Martinez1Carmen Molins-Legua2Pilar Campins-Falcó3MINTOTA Research Group, Departament de Química Analítica, Facultad de Química, Universitat de Valencia, C/Doctor Moliner 50, 46100 Burjassot, SpainMINTOTA Research Group, Departament de Química Analítica, Facultad de Química, Universitat de Valencia, C/Doctor Moliner 50, 46100 Burjassot, SpainMINTOTA Research Group, Departament de Química Analítica, Facultad de Química, Universitat de Valencia, C/Doctor Moliner 50, 46100 Burjassot, SpainMINTOTA Research Group, Departament de Química Analítica, Facultad de Química, Universitat de Valencia, C/Doctor Moliner 50, 46100 Burjassot, SpainThe potential of antioxidants in preventing several diseases has attracted great attention in recent years. Indeed, these products are part of a multi-billion industry. However, there is a lack of scientific information about safety, quality, doses, and changes over time. In the present work, a simple multisample methodology based on chemiluminiscent imaging to determine chlorogenic acid (CHLA) in green coffee samples has been proposed. The multi-chemiluminiscent response was obtained after a luminol-persulfate reaction at pH 10.8 in a multiplate followed by image capture with a charge-coupled device (CCD) camera as a readout system. The chemiluminiscent image was used as an analytical response by measuring the luminescent intensity at 0 °C with the CCD camera. Under the optimal conditions, the detection limit was 20 µM and precision was also adequate with RSD < 12%. The accuracy of the proposed system was evaluated by studying the matrix effect, using a standard addition method. Recoveries of chlorogenic acid ranged from 93–94%. The use of the CCD camera demonstrated advantages such as analysis by image inspection, portability, and easy-handling which is of particular relevance in the application for quality control in industries. Furthermore, multisample analysis was allowed by one single image saving time, energy, and cost. The proposed methodology is a promising sustainable analytical tool for quality control to ensure green coffee safety through dosage control and proper labelling preventing potential frauds.https://www.mdpi.com/2409-9279/6/1/20antioxidantschlorogenic acidchemiluminiscenceCCD cameragreen coffeeon-site |
| spellingShingle | Sergi Mallorca-Cebria Yolanda Moliner-Martinez Carmen Molins-Legua Pilar Campins-Falcó On-Site Multisample Determination of Chlorogenic Acid in Green Coffee by Chemiluminiscent Imaging Methods and Protocols antioxidants chlorogenic acid chemiluminiscence CCD camera green coffee on-site |
| title | On-Site Multisample Determination of Chlorogenic Acid in Green Coffee by Chemiluminiscent Imaging |
| title_full | On-Site Multisample Determination of Chlorogenic Acid in Green Coffee by Chemiluminiscent Imaging |
| title_fullStr | On-Site Multisample Determination of Chlorogenic Acid in Green Coffee by Chemiluminiscent Imaging |
| title_full_unstemmed | On-Site Multisample Determination of Chlorogenic Acid in Green Coffee by Chemiluminiscent Imaging |
| title_short | On-Site Multisample Determination of Chlorogenic Acid in Green Coffee by Chemiluminiscent Imaging |
| title_sort | on site multisample determination of chlorogenic acid in green coffee by chemiluminiscent imaging |
| topic | antioxidants chlorogenic acid chemiluminiscence CCD camera green coffee on-site |
| url | https://www.mdpi.com/2409-9279/6/1/20 |
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