Genome-Wide Identification and Analysis of Chitinase-Like Gene Family in <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae)
Chitinases are of great importance in chitin degradation and remodeling in insects. However, the genome-wide distribution of chitinase-like gene family in <i>Bemsia tabaci</i>, a destructive pest worldwide, is still elusive. With the help of bioinformatics, we annotated 14 genes that enc...
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MDPI AG
2021-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/3/254 |
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| author | Zhengke Peng Jun Ren Qi Su Yang Zeng Lixia Tian Shaoli Wang Qingjun Wu Pei Liang Wen Xie Youjun Zhang |
| author_facet | Zhengke Peng Jun Ren Qi Su Yang Zeng Lixia Tian Shaoli Wang Qingjun Wu Pei Liang Wen Xie Youjun Zhang |
| author_sort | Zhengke Peng |
| collection | DOAJ |
| description | Chitinases are of great importance in chitin degradation and remodeling in insects. However, the genome-wide distribution of chitinase-like gene family in <i>Bemsia tabaci</i>, a destructive pest worldwide, is still elusive. With the help of bioinformatics, we annotated 14 genes that encode putative chitinase-like proteins, including ten chitinases (Cht), three imaginal disk growth factors (<i>IDGF</i>), and one endo-β-N-acetylglucosaminidase (<i>ENGase</i>) in the genome of the whitefly, <i>B. tabaci</i>. These genes were phylogenetically grouped into eight clades, among which 13 genes were classified in the glycoside hydrolase family 18 groups and one in the <i>ENGase</i> group. Afterwards, developmental expression analysis suggested that <i>BtCht10</i>, <i>BtCht5,</i> and <i>BtCht7</i> were highly expressed in nymphal stages and exhibit similar expression patterns, implying their underlying role in nymph ecdysis. Notably, nymphs exhibited a lower rate of survival when challenged by dsRNA targeting these three genes via a nanomaterial-promoted RNAi method. In addition, silencing of <i>BtCht10</i> significantly resulted in a longer duration of development compared to control nymphs. These results indicate a key role of <i>BtCht10</i>, <i>BtCht5,</i> and <i>BtCht7</i> in <i>B. tabaci</i> nymph molting. Our research depicts the differences of chitinase-like family genes in structure and function and identified potential targets for RNAi-based whitefly management. |
| first_indexed | 2024-03-10T13:08:29Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T13:08:29Z |
| publishDate | 2021-03-01 |
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| series | Insects |
| spelling | doaj.art-2c552f90dbf64387b12c01d59f2621c72023-11-21T10:55:09ZengMDPI AGInsects2075-44502021-03-0112325410.3390/insects12030254Genome-Wide Identification and Analysis of Chitinase-Like Gene Family in <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae)Zhengke Peng0Jun Ren1Qi Su2Yang Zeng3Lixia Tian4Shaoli Wang5Qingjun Wu6Pei Liang7Wen Xie8Youjun Zhang9Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaHubei Engineering Technology Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaDepartment of Entomology, China Agricultural University, Beijing 100193, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaChitinases are of great importance in chitin degradation and remodeling in insects. However, the genome-wide distribution of chitinase-like gene family in <i>Bemsia tabaci</i>, a destructive pest worldwide, is still elusive. With the help of bioinformatics, we annotated 14 genes that encode putative chitinase-like proteins, including ten chitinases (Cht), three imaginal disk growth factors (<i>IDGF</i>), and one endo-β-N-acetylglucosaminidase (<i>ENGase</i>) in the genome of the whitefly, <i>B. tabaci</i>. These genes were phylogenetically grouped into eight clades, among which 13 genes were classified in the glycoside hydrolase family 18 groups and one in the <i>ENGase</i> group. Afterwards, developmental expression analysis suggested that <i>BtCht10</i>, <i>BtCht5,</i> and <i>BtCht7</i> were highly expressed in nymphal stages and exhibit similar expression patterns, implying their underlying role in nymph ecdysis. Notably, nymphs exhibited a lower rate of survival when challenged by dsRNA targeting these three genes via a nanomaterial-promoted RNAi method. In addition, silencing of <i>BtCht10</i> significantly resulted in a longer duration of development compared to control nymphs. These results indicate a key role of <i>BtCht10</i>, <i>BtCht5,</i> and <i>BtCht7</i> in <i>B. tabaci</i> nymph molting. Our research depicts the differences of chitinase-like family genes in structure and function and identified potential targets for RNAi-based whitefly management.https://www.mdpi.com/2075-4450/12/3/254<i>Bemisia tabaci</i>chitinaseglycosyl hydrolase familyphylogenetic analysisRNA interference |
| spellingShingle | Zhengke Peng Jun Ren Qi Su Yang Zeng Lixia Tian Shaoli Wang Qingjun Wu Pei Liang Wen Xie Youjun Zhang Genome-Wide Identification and Analysis of Chitinase-Like Gene Family in <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae) Insects <i>Bemisia tabaci</i> chitinase glycosyl hydrolase family phylogenetic analysis RNA interference |
| title | Genome-Wide Identification and Analysis of Chitinase-Like Gene Family in <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae) |
| title_full | Genome-Wide Identification and Analysis of Chitinase-Like Gene Family in <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae) |
| title_fullStr | Genome-Wide Identification and Analysis of Chitinase-Like Gene Family in <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae) |
| title_full_unstemmed | Genome-Wide Identification and Analysis of Chitinase-Like Gene Family in <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae) |
| title_short | Genome-Wide Identification and Analysis of Chitinase-Like Gene Family in <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae) |
| title_sort | genome wide identification and analysis of chitinase like gene family in i bemisia tabaci i hemiptera aleyrodidae |
| topic | <i>Bemisia tabaci</i> chitinase glycosyl hydrolase family phylogenetic analysis RNA interference |
| url | https://www.mdpi.com/2075-4450/12/3/254 |
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