Fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytes
Abstract Aims We investigated the effects of intraperitoneal injections of titanium dioxide nanoparticles (TiO2 NPs, 100 mg/kg) for 5 consecutive days on the developmental competence of murine oocytes. Furthermore, study the effects of TiO2 NPs on antioxidant and oxidative stress biomarkers, as well...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-024-03963-7 |
| _version_ | 1797219719011368960 |
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| author | Mohammed A Elmetwally Amal Helmy Ahmed Balboula Mohamed Eladl Basma Hamed Samah Lashen Shaymaa Rezk Amira Yaseen Heba Sharawy Mamdouh Hussien Samy Zabel Abdelmonem Montaser Amal Halawa |
| author_facet | Mohammed A Elmetwally Amal Helmy Ahmed Balboula Mohamed Eladl Basma Hamed Samah Lashen Shaymaa Rezk Amira Yaseen Heba Sharawy Mamdouh Hussien Samy Zabel Abdelmonem Montaser Amal Halawa |
| author_sort | Mohammed A Elmetwally |
| collection | DOAJ |
| description | Abstract Aims We investigated the effects of intraperitoneal injections of titanium dioxide nanoparticles (TiO2 NPs, 100 mg/kg) for 5 consecutive days on the developmental competence of murine oocytes. Furthermore, study the effects of TiO2 NPs on antioxidant and oxidative stress biomarkers, as well as their effects on expression of apoptotic and hypoxia inducing factor-1α (HIF1A) protein translation. Moreover, the possible ameliorating effects of intraperitoneal injections of fructose (2.75 mM/ml) was examined. Materials and methods Thirty sexually mature (8–12 weeks old; ~ 25 g body weight) female mice were used for the current study. The female mice were assigned randomly to three treatment groups: Group1 (G1) mice were injected intraperitoneal (ip) with deionized water for 5 consecutive days; Group 2 (G2) mice were injected ip with TiO2 NPs (100 mg/kg BW) for 5 consecutive days; Group 3 (G3) mice were injected ip with TiO2 NPs (100 mg/kg BW + fructose (2.75 mM) for 5 consecutive days. Results Nano-titanium significantly decreased expression of GSH, GPx, and NO, expression of MDA and TAC increased. The rates of MI, MII, GVBD and degenerated oocytes were significantly less for nano-titanium treated mice, but the rate of activated oocytes was significantly greater than those in control oocytes. TiO2 NPs significantly increased expression of apoptotic genes (BAX, Caspase 3 and P53) and HIF1A. Intraperitoneal injection of fructose (2.75 mM/kg) significantly alleviated the detrimental effects of TiO2 NPs. Transmission electron microscopy indicated that fructose mitigated adverse effects of TiO2 NPs to alter the cell surface of murine oocytes. Conclusion Results of this study suggest that the i/p infusion of fructose for consecutive 5 days enhances development of murine oocytes and decreases toxic effects of TiO2 NPs through positive effects on oxidative and antioxidant biomarkers in cumulus-oocyte complexes and effects to inhibit TiO2-induced increases in expression of apoptotic and hypoxia inducing factors. |
| first_indexed | 2024-04-24T12:38:06Z |
| format | Article |
| id | doaj.art-2c573d91d6fd437b96bb45df62eb55b9 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-24T12:38:06Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-2c573d91d6fd437b96bb45df62eb55b92024-04-07T11:21:48ZengBMCBMC Veterinary Research1746-61482024-04-0120111110.1186/s12917-024-03963-7Fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytesMohammed A Elmetwally0Amal Helmy1Ahmed Balboula2Mohamed Eladl3Basma Hamed4Samah Lashen5Shaymaa Rezk6Amira Yaseen7Heba Sharawy8Mamdouh Hussien9Samy Zabel10Abdelmonem Montaser11Amal Halawa12Department of Theriogenology, Center for Reproductive Biotechnology, Faculty of Veterinary Medicine, Mansoura UniversityFertility care center, Obstetrics and Gynecology department, faculty of medicine, Mansoura UniversityDepartment of animal science, University of MissouriDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Mansoura UniversityMedical research center, faculty of medicine, Mansoura UniversityDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura UniversityFertility care center, Obstetrics and Gynecology department, faculty of medicine, Mansoura UniversityDepartment of Theriogenology, Center for Reproductive Biotechnology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Theriogenology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Theriogenology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Theriogenology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Mansoura UniversityAbstract Aims We investigated the effects of intraperitoneal injections of titanium dioxide nanoparticles (TiO2 NPs, 100 mg/kg) for 5 consecutive days on the developmental competence of murine oocytes. Furthermore, study the effects of TiO2 NPs on antioxidant and oxidative stress biomarkers, as well as their effects on expression of apoptotic and hypoxia inducing factor-1α (HIF1A) protein translation. Moreover, the possible ameliorating effects of intraperitoneal injections of fructose (2.75 mM/ml) was examined. Materials and methods Thirty sexually mature (8–12 weeks old; ~ 25 g body weight) female mice were used for the current study. The female mice were assigned randomly to three treatment groups: Group1 (G1) mice were injected intraperitoneal (ip) with deionized water for 5 consecutive days; Group 2 (G2) mice were injected ip with TiO2 NPs (100 mg/kg BW) for 5 consecutive days; Group 3 (G3) mice were injected ip with TiO2 NPs (100 mg/kg BW + fructose (2.75 mM) for 5 consecutive days. Results Nano-titanium significantly decreased expression of GSH, GPx, and NO, expression of MDA and TAC increased. The rates of MI, MII, GVBD and degenerated oocytes were significantly less for nano-titanium treated mice, but the rate of activated oocytes was significantly greater than those in control oocytes. TiO2 NPs significantly increased expression of apoptotic genes (BAX, Caspase 3 and P53) and HIF1A. Intraperitoneal injection of fructose (2.75 mM/kg) significantly alleviated the detrimental effects of TiO2 NPs. Transmission electron microscopy indicated that fructose mitigated adverse effects of TiO2 NPs to alter the cell surface of murine oocytes. Conclusion Results of this study suggest that the i/p infusion of fructose for consecutive 5 days enhances development of murine oocytes and decreases toxic effects of TiO2 NPs through positive effects on oxidative and antioxidant biomarkers in cumulus-oocyte complexes and effects to inhibit TiO2-induced increases in expression of apoptotic and hypoxia inducing factors.https://doi.org/10.1186/s12917-024-03963-7Murine oocytesTiO2 NPsFructoseApoptosisAntioxidantOxidative stress |
| spellingShingle | Mohammed A Elmetwally Amal Helmy Ahmed Balboula Mohamed Eladl Basma Hamed Samah Lashen Shaymaa Rezk Amira Yaseen Heba Sharawy Mamdouh Hussien Samy Zabel Abdelmonem Montaser Amal Halawa Fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytes BMC Veterinary Research Murine oocytes TiO2 NPs Fructose Apoptosis Antioxidant Oxidative stress |
| title | Fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytes |
| title_full | Fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytes |
| title_fullStr | Fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytes |
| title_full_unstemmed | Fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytes |
| title_short | Fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytes |
| title_sort | fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytes |
| topic | Murine oocytes TiO2 NPs Fructose Apoptosis Antioxidant Oxidative stress |
| url | https://doi.org/10.1186/s12917-024-03963-7 |
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