Detection of SARS-CoV-2 Genome in Stool and Plasma Samples of Laboratory Confirmed Iranian COVID-19 Patients
Coronavirus disease 2019 (COVID19), caused by the severe acute respiratory syndrome coronavirus 2 (SARSCoV2), was first discovered in China in late 2019 and quickly spread worldwide. Although nasopharyngeal swab sampling is still the most popular approach identify SARS-CoV-2 carriers, other body sam...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.865129/full |
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| author | Mobin Makhmalbaf Mobin Makhmalbaf Seyed Masoud Hosseini Hamid Asadzadeh Aghdaei Mahsa Saeedi Niasar Shahrzad Shoraka Abbas Yadegar Shaghayegh Baradaran Ghavami Shabnam Shahrokh Mohammadreza Moshari Habib Malekpour Mohammad Reza Zali Seyed Reza Mohebbi |
| author_facet | Mobin Makhmalbaf Mobin Makhmalbaf Seyed Masoud Hosseini Hamid Asadzadeh Aghdaei Mahsa Saeedi Niasar Shahrzad Shoraka Abbas Yadegar Shaghayegh Baradaran Ghavami Shabnam Shahrokh Mohammadreza Moshari Habib Malekpour Mohammad Reza Zali Seyed Reza Mohebbi |
| author_sort | Mobin Makhmalbaf |
| collection | DOAJ |
| description | Coronavirus disease 2019 (COVID19), caused by the severe acute respiratory syndrome coronavirus 2 (SARSCoV2), was first discovered in China in late 2019 and quickly spread worldwide. Although nasopharyngeal swab sampling is still the most popular approach identify SARS-CoV-2 carriers, other body samples may reveal the virus genome, indicating the potential for virus transmission via non-respiratory samples. In this study, researchers looked at the presence and degree of SARS-CoV-2 genome in stool and plasma samples from 191 Iranian COVID-19 patients, and looked for a link between these results and the severity of their disease. SARS-CoV-2 RNA shedding in feces and plasma of COVID-19 patients was assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Medical data were collected and evaluated, including Clinical features, demographics, radiological, and laboratory findings of the patients. Plasma samples from 117 confirmed laboratory patients were evaluated and 24 out of 117 patients (20.51%) tested positive for SARS-COV-2 RNA. Besides, 20 out of 74 patients (27.03%) tested positive for SARS-COV-2 RNA in stool samples. There seems to be no relationship between the presence of SARS-CoV-2 genome in fecal and plasma samples of Covid-19 patients and the severity of illness. We provide evidence of the SARS-CoV-2 genome presence in stool and plasma samples of Iranian COVID-19 patients. |
| first_indexed | 2024-04-13T19:46:05Z |
| format | Article |
| id | doaj.art-2c5d2e30c35149de86ed19c8f90587bb |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-13T19:46:05Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-2c5d2e30c35149de86ed19c8f90587bb2022-12-22T02:32:44ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-06-01910.3389/fmolb.2022.865129865129Detection of SARS-CoV-2 Genome in Stool and Plasma Samples of Laboratory Confirmed Iranian COVID-19 PatientsMobin Makhmalbaf0Mobin Makhmalbaf1Seyed Masoud Hosseini2Hamid Asadzadeh Aghdaei3Mahsa Saeedi Niasar4Shahrzad Shoraka5Abbas Yadegar6Shaghayegh Baradaran Ghavami7Shabnam Shahrokh8Mohammadreza Moshari9Habib Malekpour10Mohammad Reza Zali11Seyed Reza Mohebbi12Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, IranDepartment of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, IranBasic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IranGastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IranGastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IranFoodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IranBasic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IranGastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Anesthesiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranResearch and Development Center, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IranGastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IranGastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IranCoronavirus disease 2019 (COVID19), caused by the severe acute respiratory syndrome coronavirus 2 (SARSCoV2), was first discovered in China in late 2019 and quickly spread worldwide. Although nasopharyngeal swab sampling is still the most popular approach identify SARS-CoV-2 carriers, other body samples may reveal the virus genome, indicating the potential for virus transmission via non-respiratory samples. In this study, researchers looked at the presence and degree of SARS-CoV-2 genome in stool and plasma samples from 191 Iranian COVID-19 patients, and looked for a link between these results and the severity of their disease. SARS-CoV-2 RNA shedding in feces and plasma of COVID-19 patients was assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Medical data were collected and evaluated, including Clinical features, demographics, radiological, and laboratory findings of the patients. Plasma samples from 117 confirmed laboratory patients were evaluated and 24 out of 117 patients (20.51%) tested positive for SARS-COV-2 RNA. Besides, 20 out of 74 patients (27.03%) tested positive for SARS-COV-2 RNA in stool samples. There seems to be no relationship between the presence of SARS-CoV-2 genome in fecal and plasma samples of Covid-19 patients and the severity of illness. We provide evidence of the SARS-CoV-2 genome presence in stool and plasma samples of Iranian COVID-19 patients.https://www.frontiersin.org/articles/10.3389/fmolb.2022.865129/fullSARS-CoV-2COVID-19RT-qPCRplasmafecesCOVID-19 nucleic acid testing |
| spellingShingle | Mobin Makhmalbaf Mobin Makhmalbaf Seyed Masoud Hosseini Hamid Asadzadeh Aghdaei Mahsa Saeedi Niasar Shahrzad Shoraka Abbas Yadegar Shaghayegh Baradaran Ghavami Shabnam Shahrokh Mohammadreza Moshari Habib Malekpour Mohammad Reza Zali Seyed Reza Mohebbi Detection of SARS-CoV-2 Genome in Stool and Plasma Samples of Laboratory Confirmed Iranian COVID-19 Patients Frontiers in Molecular Biosciences SARS-CoV-2 COVID-19 RT-qPCR plasma feces COVID-19 nucleic acid testing |
| title | Detection of SARS-CoV-2 Genome in Stool and Plasma Samples of Laboratory Confirmed Iranian COVID-19 Patients |
| title_full | Detection of SARS-CoV-2 Genome in Stool and Plasma Samples of Laboratory Confirmed Iranian COVID-19 Patients |
| title_fullStr | Detection of SARS-CoV-2 Genome in Stool and Plasma Samples of Laboratory Confirmed Iranian COVID-19 Patients |
| title_full_unstemmed | Detection of SARS-CoV-2 Genome in Stool and Plasma Samples of Laboratory Confirmed Iranian COVID-19 Patients |
| title_short | Detection of SARS-CoV-2 Genome in Stool and Plasma Samples of Laboratory Confirmed Iranian COVID-19 Patients |
| title_sort | detection of sars cov 2 genome in stool and plasma samples of laboratory confirmed iranian covid 19 patients |
| topic | SARS-CoV-2 COVID-19 RT-qPCR plasma feces COVID-19 nucleic acid testing |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.865129/full |
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