In Vivo Human Somitogenesis Guides Somite Development from hPSCs
Somites form during embryonic development and give rise to unique cell and tissue types, such as skeletal muscles and bones and cartilage of the vertebrae. Using somitogenesis-stage human embryos, we performed transcriptomic profiling of human presomitic mesoderm as well as nascent and developed som...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-02-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717300955 |
| _version_ | 1818970855696236544 |
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| author | Haibin Xi Wakana Fujiwara Karen Gonzalez Majib Jan Simone Liebscher Ben Van Handel Katja Schenke-Layland April D. Pyle |
| author_facet | Haibin Xi Wakana Fujiwara Karen Gonzalez Majib Jan Simone Liebscher Ben Van Handel Katja Schenke-Layland April D. Pyle |
| author_sort | Haibin Xi |
| collection | DOAJ |
| description | Somites form during embryonic development and give rise to unique cell and tissue types, such as skeletal muscles and bones and cartilage of the vertebrae. Using somitogenesis-stage human embryos, we performed transcriptomic profiling of human presomitic mesoderm as well as nascent and developed somites. In addition to conserved pathways such as WNT-β-catenin, we also identified BMP and transforming growth factor β (TGF-β) signaling as major regulators unique to human somitogenesis. This information enabled us to develop an efficient protocol to derive somite cells in vitro from human pluripotent stem cells (hPSCs). Importantly, the in-vitro-differentiating cells progressively expressed markers of the distinct developmental stages that are known to occur during in vivo somitogenesis. Furthermore, when subjected to lineage-specific differentiation conditions, the hPSC-derived somite cells were multipotent in generating somite derivatives, including skeletal myocytes, osteocytes, and chondrocytes. This work improves our understanding of human somitogenesis and may enhance our ability to treat diseases affecting somite derivatives. |
| first_indexed | 2024-12-20T14:43:07Z |
| format | Article |
| id | doaj.art-2c5d8ad1d37f4e2fa142ed1be03ca66e |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-20T14:43:07Z |
| publishDate | 2017-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-2c5d8ad1d37f4e2fa142ed1be03ca66e2022-12-21T19:37:12ZengElsevierCell Reports2211-12472017-02-011861573158510.1016/j.celrep.2017.01.040In Vivo Human Somitogenesis Guides Somite Development from hPSCsHaibin Xi0Wakana Fujiwara1Karen Gonzalez2Majib Jan3Simone Liebscher4Ben Van Handel5Katja Schenke-Layland6April D. Pyle7Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USADepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USADepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USADepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USADepartment of Women’s Health, Research Institute for Women’s Health, Eberhard Karls University Tübingen, 72074 Tübingen, GermanyDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USADepartment of Women’s Health, Research Institute for Women’s Health, Eberhard Karls University Tübingen, 72074 Tübingen, GermanyDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USASomites form during embryonic development and give rise to unique cell and tissue types, such as skeletal muscles and bones and cartilage of the vertebrae. Using somitogenesis-stage human embryos, we performed transcriptomic profiling of human presomitic mesoderm as well as nascent and developed somites. In addition to conserved pathways such as WNT-β-catenin, we also identified BMP and transforming growth factor β (TGF-β) signaling as major regulators unique to human somitogenesis. This information enabled us to develop an efficient protocol to derive somite cells in vitro from human pluripotent stem cells (hPSCs). Importantly, the in-vitro-differentiating cells progressively expressed markers of the distinct developmental stages that are known to occur during in vivo somitogenesis. Furthermore, when subjected to lineage-specific differentiation conditions, the hPSC-derived somite cells were multipotent in generating somite derivatives, including skeletal myocytes, osteocytes, and chondrocytes. This work improves our understanding of human somitogenesis and may enhance our ability to treat diseases affecting somite derivatives.http://www.sciencedirect.com/science/article/pii/S2211124717300955human pluripotent stem cellssomiteskeletal myogenesisosteogenesischondrogenesisdifferentiationdevelopment |
| spellingShingle | Haibin Xi Wakana Fujiwara Karen Gonzalez Majib Jan Simone Liebscher Ben Van Handel Katja Schenke-Layland April D. Pyle In Vivo Human Somitogenesis Guides Somite Development from hPSCs Cell Reports human pluripotent stem cells somite skeletal myogenesis osteogenesis chondrogenesis differentiation development |
| title | In Vivo Human Somitogenesis Guides Somite Development from hPSCs |
| title_full | In Vivo Human Somitogenesis Guides Somite Development from hPSCs |
| title_fullStr | In Vivo Human Somitogenesis Guides Somite Development from hPSCs |
| title_full_unstemmed | In Vivo Human Somitogenesis Guides Somite Development from hPSCs |
| title_short | In Vivo Human Somitogenesis Guides Somite Development from hPSCs |
| title_sort | in vivo human somitogenesis guides somite development from hpscs |
| topic | human pluripotent stem cells somite skeletal myogenesis osteogenesis chondrogenesis differentiation development |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717300955 |
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