Evaluation of eugenol as anesthetic in Cat-fish (Clarias gariepinus) with special reference to biochemical and histopathological alterations
The anesthetic, biochemical and pathological effects of eugenol were evaluated in the African cat-fish Clarias gariepinus in comparison with MS-222. Aquous exposure of Clarias gariepinus to eugenol at concentration of 62.5 – 250 ) g / ml showed short interval to induction than MS-222. On the other h...
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Faculty of Veterinary Medicine, Beni-Suef University
2005-12-01
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| Series: | Journal of Veterinary Medical Research |
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| Online Access: | https://jvmr.journals.ekb.eg/article_77941.html |
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| author | M. A. F. Abdel-Fattah Amina A. Dessouki T. Ramadan Mona M. Ismail |
| author_facet | M. A. F. Abdel-Fattah Amina A. Dessouki T. Ramadan Mona M. Ismail |
| author_sort | M. A. F. Abdel-Fattah |
| collection | DOAJ |
| description | The anesthetic, biochemical and pathological effects of eugenol were evaluated in the African cat-fish Clarias gariepinus in comparison with MS-222. Aquous exposure of Clarias gariepinus to eugenol at concentration of 62.5 – 250 ) g / ml showed short interval to induction than MS-222. On the other hand, eugenol provided longer time for recovery (8.28 min) at higher concentration (250 )g / ml) than MS-222 (5.26 min) but no differences were observed at lower concentrations between both agents. Mortality rate of 46.5 and 33.3 % was recorded for eugenol and MS-222, respectively at 250 )g / ml. Exposure of fish to 250 )g / ml of eugenol or MS-222 resulted in significant increases in serum levels of creatinine, urea and uric acid and decrease in total protein and albumin. Significant increases in AST, ALT and Ca levels were recorded only on exposure to eugenol 250 )g / ml. Histopathological examinations showed congestion of gill blood vessels, diffuse vacuolar degeneration and necrosis of the epithelium lining of the secondary lamellae of the gill filaments, degeneration and focal necrosis of renal tubules in kidneys, necrosis and deposition of hemosiderin pigment in the macrophages of spleen which is indicative for hemorrhages and hemolysis as well as mild catarrhal enteritis of intestine of fish groups exposed to 250 ) g / ml of both agents. Moreover, massive fatty and degenerative changes in liver were observed only in fish exposed to 250 )g / ml of eugenol. On the other hand, lower concentrations of both agents did not differ from those of control fish. In conclusion, it appears that eugenol at lower and medium concentrations could produce an anesthesia characterized by rapid induction and rational recovery without significant changes in tissues and blood chemistry. Care should be taken on using high concentrations of eugenol as ventilatory failure may occur |
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| issn | 2357-0512 2357-0520 |
| language | English |
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| publishDate | 2005-12-01 |
| publisher | Faculty of Veterinary Medicine, Beni-Suef University |
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| series | Journal of Veterinary Medical Research |
| spelling | doaj.art-2c5e4c4781294235870dbdc57375572f2022-12-21T17:24:00ZengFaculty of Veterinary Medicine, Beni-Suef UniversityJournal of Veterinary Medical Research2357-05122357-05202005-12-01152116122Evaluation of eugenol as anesthetic in Cat-fish (Clarias gariepinus) with special reference to biochemical and histopathological alterationsM. A. F. Abdel-Fattah0 Amina A. Dessouki1T. Ramadan2 Mona M. Ismail3Departments of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptPathology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptClinical Pathology Department , Faculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptFish Diseases and Management, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptThe anesthetic, biochemical and pathological effects of eugenol were evaluated in the African cat-fish Clarias gariepinus in comparison with MS-222. Aquous exposure of Clarias gariepinus to eugenol at concentration of 62.5 – 250 ) g / ml showed short interval to induction than MS-222. On the other hand, eugenol provided longer time for recovery (8.28 min) at higher concentration (250 )g / ml) than MS-222 (5.26 min) but no differences were observed at lower concentrations between both agents. Mortality rate of 46.5 and 33.3 % was recorded for eugenol and MS-222, respectively at 250 )g / ml. Exposure of fish to 250 )g / ml of eugenol or MS-222 resulted in significant increases in serum levels of creatinine, urea and uric acid and decrease in total protein and albumin. Significant increases in AST, ALT and Ca levels were recorded only on exposure to eugenol 250 )g / ml. Histopathological examinations showed congestion of gill blood vessels, diffuse vacuolar degeneration and necrosis of the epithelium lining of the secondary lamellae of the gill filaments, degeneration and focal necrosis of renal tubules in kidneys, necrosis and deposition of hemosiderin pigment in the macrophages of spleen which is indicative for hemorrhages and hemolysis as well as mild catarrhal enteritis of intestine of fish groups exposed to 250 ) g / ml of both agents. Moreover, massive fatty and degenerative changes in liver were observed only in fish exposed to 250 )g / ml of eugenol. On the other hand, lower concentrations of both agents did not differ from those of control fish. In conclusion, it appears that eugenol at lower and medium concentrations could produce an anesthesia characterized by rapid induction and rational recovery without significant changes in tissues and blood chemistry. Care should be taken on using high concentrations of eugenol as ventilatory failure may occurhttps://jvmr.journals.ekb.eg/article_77941.htmlevaluation; eugenol; anesthetic; cat-fish; clarias gariepinus; biochemical; histopathological |
| spellingShingle | M. A. F. Abdel-Fattah Amina A. Dessouki T. Ramadan Mona M. Ismail Evaluation of eugenol as anesthetic in Cat-fish (Clarias gariepinus) with special reference to biochemical and histopathological alterations Journal of Veterinary Medical Research evaluation; eugenol; anesthetic; cat-fish; clarias gariepinus; biochemical; histopathological |
| title | Evaluation of eugenol as anesthetic in Cat-fish (Clarias gariepinus) with special reference to biochemical and histopathological alterations |
| title_full | Evaluation of eugenol as anesthetic in Cat-fish (Clarias gariepinus) with special reference to biochemical and histopathological alterations |
| title_fullStr | Evaluation of eugenol as anesthetic in Cat-fish (Clarias gariepinus) with special reference to biochemical and histopathological alterations |
| title_full_unstemmed | Evaluation of eugenol as anesthetic in Cat-fish (Clarias gariepinus) with special reference to biochemical and histopathological alterations |
| title_short | Evaluation of eugenol as anesthetic in Cat-fish (Clarias gariepinus) with special reference to biochemical and histopathological alterations |
| title_sort | evaluation of eugenol as anesthetic in cat fish clarias gariepinus with special reference to biochemical and histopathological alterations |
| topic | evaluation; eugenol; anesthetic; cat-fish; clarias gariepinus; biochemical; histopathological |
| url | https://jvmr.journals.ekb.eg/article_77941.html |
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