Assessment of the <i>In Vitro</i> and <i>In Vivo</i> Antitumor Activity of Hemocyanins from <i>Helix aspersa</i>, <i>Helix lucorum</i>, and <i>Rapana venosa</i> in a Graffi Myeloid Tumor Model
Hemocyanins are oxygen-transporting glycoproteins in the hemolymph of some invertebrate species that attracted scientific interest as potential anticancer agents. The present study aims to assess the <i>in vitro</i> and <i>in vivo</i> anticancer activity of hemocyanins isolat...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/6/1545 |
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| author | Ani Georgieva Katerina Todorova Ivan Iliev Valeria Dilcheva Ivelin Vladov Svetlozara Petkova Aleksandar Dolashki Lyudmila Velkova Pavlina Dolashka Reneta Toshkova |
| author_facet | Ani Georgieva Katerina Todorova Ivan Iliev Valeria Dilcheva Ivelin Vladov Svetlozara Petkova Aleksandar Dolashki Lyudmila Velkova Pavlina Dolashka Reneta Toshkova |
| author_sort | Ani Georgieva |
| collection | DOAJ |
| description | Hemocyanins are oxygen-transporting glycoproteins in the hemolymph of some invertebrate species that attracted scientific interest as potential anticancer agents. The present study aims to assess the <i>in vitro</i> and <i>in vivo</i> anticancer activity of hemocyanins isolated from <i>Helix aspersa</i>, <i>Helix lucorum</i>, and <i>Rapana venosa</i> in the Graffi myeloid tumor model. The <i>in vitro</i> antitumor activity of the hemocyanins was determined by a MTT test and cytomorphological analysis by fluorescent and transmission electron microscopy. The <i>in vivo</i> effects of the hemocyanins were examined in hamsters transplanted with Graffi tumor. The serum antibody titers against the tested hemocyanins and tumor antigen were determined by ELISA. Histopathological assessment of the morphological features related to antitumor effect, immune system response, and toxicity in some internal organs was performed. The results of <i>in vitro</i> studies indicated that the tested hemocyanins induced significant antiproliferative and apoptogenic effects. The <i>in vivo</i> investigations demonstrated a protective antitumor effect, expressed in reduced transplantability, suppression of tumor growth and metastasis, reduced mortality, prolonged survival time, and absence of toxic side effects. The present study indicated that the antitumor activity of the studied hemocyanins was due to both immune stimulation and direct effects on the tumor cells, and they displayed their potential as therapeutic agents against hematological malignances. |
| first_indexed | 2024-03-11T02:44:30Z |
| format | Article |
| id | doaj.art-2c5ea03905f14d0ea27cb7cb14b222ac |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T02:44:30Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-2c5ea03905f14d0ea27cb7cb14b222ac2023-11-18T09:24:52ZengMDPI AGBiomedicines2227-90592023-05-01116154510.3390/biomedicines11061545Assessment of the <i>In Vitro</i> and <i>In Vivo</i> Antitumor Activity of Hemocyanins from <i>Helix aspersa</i>, <i>Helix lucorum</i>, and <i>Rapana venosa</i> in a Graffi Myeloid Tumor ModelAni Georgieva0Katerina Todorova1Ivan Iliev2Valeria Dilcheva3Ivelin Vladov4Svetlozara Petkova5Aleksandar Dolashki6Lyudmila Velkova7Pavlina Dolashka8Reneta Toshkova9Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, BulgariaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, BulgariaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, BulgariaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, BulgariaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, BulgariaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 9, 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 9, 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 9, 1113 Sofia, BulgariaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, BulgariaHemocyanins are oxygen-transporting glycoproteins in the hemolymph of some invertebrate species that attracted scientific interest as potential anticancer agents. The present study aims to assess the <i>in vitro</i> and <i>in vivo</i> anticancer activity of hemocyanins isolated from <i>Helix aspersa</i>, <i>Helix lucorum</i>, and <i>Rapana venosa</i> in the Graffi myeloid tumor model. The <i>in vitro</i> antitumor activity of the hemocyanins was determined by a MTT test and cytomorphological analysis by fluorescent and transmission electron microscopy. The <i>in vivo</i> effects of the hemocyanins were examined in hamsters transplanted with Graffi tumor. The serum antibody titers against the tested hemocyanins and tumor antigen were determined by ELISA. Histopathological assessment of the morphological features related to antitumor effect, immune system response, and toxicity in some internal organs was performed. The results of <i>in vitro</i> studies indicated that the tested hemocyanins induced significant antiproliferative and apoptogenic effects. The <i>in vivo</i> investigations demonstrated a protective antitumor effect, expressed in reduced transplantability, suppression of tumor growth and metastasis, reduced mortality, prolonged survival time, and absence of toxic side effects. The present study indicated that the antitumor activity of the studied hemocyanins was due to both immune stimulation and direct effects on the tumor cells, and they displayed their potential as therapeutic agents against hematological malignances.https://www.mdpi.com/2227-9059/11/6/1545hemocyaninsGraffi myeloid tumorantitumor activityimmunostimulating activity |
| spellingShingle | Ani Georgieva Katerina Todorova Ivan Iliev Valeria Dilcheva Ivelin Vladov Svetlozara Petkova Aleksandar Dolashki Lyudmila Velkova Pavlina Dolashka Reneta Toshkova Assessment of the <i>In Vitro</i> and <i>In Vivo</i> Antitumor Activity of Hemocyanins from <i>Helix aspersa</i>, <i>Helix lucorum</i>, and <i>Rapana venosa</i> in a Graffi Myeloid Tumor Model Biomedicines hemocyanins Graffi myeloid tumor antitumor activity immunostimulating activity |
| title | Assessment of the <i>In Vitro</i> and <i>In Vivo</i> Antitumor Activity of Hemocyanins from <i>Helix aspersa</i>, <i>Helix lucorum</i>, and <i>Rapana venosa</i> in a Graffi Myeloid Tumor Model |
| title_full | Assessment of the <i>In Vitro</i> and <i>In Vivo</i> Antitumor Activity of Hemocyanins from <i>Helix aspersa</i>, <i>Helix lucorum</i>, and <i>Rapana venosa</i> in a Graffi Myeloid Tumor Model |
| title_fullStr | Assessment of the <i>In Vitro</i> and <i>In Vivo</i> Antitumor Activity of Hemocyanins from <i>Helix aspersa</i>, <i>Helix lucorum</i>, and <i>Rapana venosa</i> in a Graffi Myeloid Tumor Model |
| title_full_unstemmed | Assessment of the <i>In Vitro</i> and <i>In Vivo</i> Antitumor Activity of Hemocyanins from <i>Helix aspersa</i>, <i>Helix lucorum</i>, and <i>Rapana venosa</i> in a Graffi Myeloid Tumor Model |
| title_short | Assessment of the <i>In Vitro</i> and <i>In Vivo</i> Antitumor Activity of Hemocyanins from <i>Helix aspersa</i>, <i>Helix lucorum</i>, and <i>Rapana venosa</i> in a Graffi Myeloid Tumor Model |
| title_sort | assessment of the i in vitro i and i in vivo i antitumor activity of hemocyanins from i helix aspersa i i helix lucorum i and i rapana venosa i in a graffi myeloid tumor model |
| topic | hemocyanins Graffi myeloid tumor antitumor activity immunostimulating activity |
| url | https://www.mdpi.com/2227-9059/11/6/1545 |
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