Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution
A fed-batch system was modified for the enzymatic production of Oleic Acid Ethyl Ester (OAEE) using rice bran (Oryza sativa) lipase by retaining the substrate molar ratio (ethanol/oleic acid) at 2.05:1 during the reaction. It resulted in an increase in the ester conversion of up to 76.8% in the firs...
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| Format: | Article |
| Language: | English |
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Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)
2015-12-01
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| Series: | Bulletin of Chemical Reaction Engineering & Catalysis |
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| Online Access: | https://journal.bcrec.id/index.php/bcrec/article/view/19335 |
| _version_ | 1827810217412263936 |
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| author | Indro Prastowo Chusnul Hidayat Pudji Hastuti |
| author_facet | Indro Prastowo Chusnul Hidayat Pudji Hastuti |
| author_sort | Indro Prastowo |
| collection | DOAJ |
| description | A fed-batch system was modified for the enzymatic production of Oleic Acid Ethyl Ester (OAEE) using rice bran (Oryza sativa) lipase by retaining the substrate molar ratio (ethanol/oleic acid) at 2.05:1 during the reaction. It resulted in an increase in the ester conversion of up to 76.8% in the first 6 h of the reaction, which was then followed by a decrease from 76.8% to 22.9% in 6 h later. The production of water in the reaction system also showed a similar trend. The water was hypothesized to lead lipase to reverse the reaction which resulted in a decrease in both (water and esters) in the last 6 h of the reac- tion. In order to overcome the problem, zeolite powder (25 and 50 mg/mL) were added into the reaction system at 5 h of the reaction. As the result, the final ester conversions increased drastically up to 90 - 95.7%. Thus, the combination of a constant substrate molar ratio (ethanol/oleic acid) during the reaction (at 2.05:1) with the addition of zeolite powder (25 and 50 mg/mL) to the reaction system at 5 h is effective for the enzymatic synthesis of OAEE. Copyright © 2015 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0). |
| first_indexed | 2024-03-11T22:49:30Z |
| format | Article |
| id | doaj.art-2c5f545235404e54aa35c66f02e7b171 |
| institution | Directory Open Access Journal |
| issn | 1978-2993 |
| language | English |
| last_indexed | 2024-03-11T22:49:30Z |
| publishDate | 2015-12-01 |
| publisher | Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS) |
| record_format | Article |
| series | Bulletin of Chemical Reaction Engineering & Catalysis |
| spelling | doaj.art-2c5f545235404e54aa35c66f02e7b1712023-09-22T03:53:06ZengMasyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)Bulletin of Chemical Reaction Engineering & Catalysis1978-29932015-12-0110323023610.9767/bcrec.10.3.8511.230-2368058Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its SolutionIndro Prastowo0Chusnul Hidayat1Pudji Hastuti2Postgraduate Program in Biotechnology, Postgraduate School, Gadjah Mada University, Jl. Teknika Utara, Barek Yogyakarta 55281, IndonesiaPostgraduate Program in Biotechnology, Postgraduate School, Gadjah Mada University, Jl. Teknika Utara, Barek Yogyakarta 55281, IndonesiaDepartment of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jl. Sosio Justisia, Bulaksumur, Yogyakarta 55281, IndonesiaA fed-batch system was modified for the enzymatic production of Oleic Acid Ethyl Ester (OAEE) using rice bran (Oryza sativa) lipase by retaining the substrate molar ratio (ethanol/oleic acid) at 2.05:1 during the reaction. It resulted in an increase in the ester conversion of up to 76.8% in the first 6 h of the reaction, which was then followed by a decrease from 76.8% to 22.9% in 6 h later. The production of water in the reaction system also showed a similar trend. The water was hypothesized to lead lipase to reverse the reaction which resulted in a decrease in both (water and esters) in the last 6 h of the reac- tion. In order to overcome the problem, zeolite powder (25 and 50 mg/mL) were added into the reaction system at 5 h of the reaction. As the result, the final ester conversions increased drastically up to 90 - 95.7%. Thus, the combination of a constant substrate molar ratio (ethanol/oleic acid) during the reaction (at 2.05:1) with the addition of zeolite powder (25 and 50 mg/mL) to the reaction system at 5 h is effective for the enzymatic synthesis of OAEE. Copyright © 2015 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).https://journal.bcrec.id/index.php/bcrec/article/view/19335fed-batch systemrice bran (oryza sativa) lipasesubstrate molar ratio (ethanol/oleic acid)waterzeolite powder |
| spellingShingle | Indro Prastowo Chusnul Hidayat Pudji Hastuti Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution Bulletin of Chemical Reaction Engineering & Catalysis fed-batch system rice bran (oryza sativa) lipase substrate molar ratio (ethanol/oleic acid) water zeolite powder |
| title | Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution |
| title_full | Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution |
| title_fullStr | Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution |
| title_full_unstemmed | Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution |
| title_short | Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution |
| title_sort | production of oleic acid ethyl ester catalyzed by crude rice bran oryza sativa lipase in a modified fed batch system a problem and its solution |
| topic | fed-batch system rice bran (oryza sativa) lipase substrate molar ratio (ethanol/oleic acid) water zeolite powder |
| url | https://journal.bcrec.id/index.php/bcrec/article/view/19335 |
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