Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease
<p>Abstract</p> <p>Background</p> <p>Kashin-Beck Disease (KBD) is an endemic osteochondropathy. Mycotoxins are believed to play an important role in the pathogenesis of KBD. Because the molecular mechanism of mycotoxin-induced cartilage lesions remains unclear, there is...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | BMC Musculoskeletal Disorders |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2474/13/130 |
| _version_ | 1818082176361562112 |
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| author | Zhang Feng Guo Xiong Wang Weizhuo Wu Shixun Ma Weijuan Yan Hua |
| author_facet | Zhang Feng Guo Xiong Wang Weizhuo Wu Shixun Ma Weijuan Yan Hua |
| author_sort | Zhang Feng |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Kashin-Beck Disease (KBD) is an endemic osteochondropathy. Mycotoxins are believed to play an important role in the pathogenesis of KBD. Because the molecular mechanism of mycotoxin-induced cartilage lesions remains unclear, there is not effective treatment for KBD now. To identify key genes involved in the mycotoxin-induced cartilage lesions, we compared the expression profiles of mycotoxin-related genes (MRG) between KBD cartilage and healthy cartilage.</p> <p>Methods</p> <p>Total RNA was isolated from cartilage samples, following by being amplified, labeled and hybridized to Agilent human whole genome microarray chip. qRT-PCR was conducted to validate the microarray data. 1,167 MRG were derived from the environmentally related genomic database Toxicogenomics. The microarray data of MRG was subjected to single gene and gene ontology (GO) expression analysis for identifying differently expressed genes and GO.</p> <p>Results</p> <p>We identified 7 up-regulated MRG and 2 down-regulated MRG in KBD cartilage, involved in collagen, apoptosis, metabolism and growth & development. GO expression analysis found that 4 apoptosis-related GO and 5 growth & development-related GO were significantly up-regulated in KBD cartilage.</p> <p>Conclusions</p> <p>Based on the results of previous and our studies, we suggest that mycotoxins might contribute to the development of KBD through dysfunction of MRG involved in collagen, apoptosis and growth & development in cartilage.</p> |
| first_indexed | 2024-12-10T19:17:56Z |
| format | Article |
| id | doaj.art-2c626cc9a0a64840ab6d037fc2822adc |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-10T19:17:56Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-2c626cc9a0a64840ab6d037fc2822adc2022-12-22T01:36:32ZengBMCBMC Musculoskeletal Disorders1471-24742012-07-0113113010.1186/1471-2474-13-130Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck diseaseZhang FengGuo XiongWang WeizhuoWu ShixunMa WeijuanYan Hua<p>Abstract</p> <p>Background</p> <p>Kashin-Beck Disease (KBD) is an endemic osteochondropathy. Mycotoxins are believed to play an important role in the pathogenesis of KBD. Because the molecular mechanism of mycotoxin-induced cartilage lesions remains unclear, there is not effective treatment for KBD now. To identify key genes involved in the mycotoxin-induced cartilage lesions, we compared the expression profiles of mycotoxin-related genes (MRG) between KBD cartilage and healthy cartilage.</p> <p>Methods</p> <p>Total RNA was isolated from cartilage samples, following by being amplified, labeled and hybridized to Agilent human whole genome microarray chip. qRT-PCR was conducted to validate the microarray data. 1,167 MRG were derived from the environmentally related genomic database Toxicogenomics. The microarray data of MRG was subjected to single gene and gene ontology (GO) expression analysis for identifying differently expressed genes and GO.</p> <p>Results</p> <p>We identified 7 up-regulated MRG and 2 down-regulated MRG in KBD cartilage, involved in collagen, apoptosis, metabolism and growth & development. GO expression analysis found that 4 apoptosis-related GO and 5 growth & development-related GO were significantly up-regulated in KBD cartilage.</p> <p>Conclusions</p> <p>Based on the results of previous and our studies, we suggest that mycotoxins might contribute to the development of KBD through dysfunction of MRG involved in collagen, apoptosis and growth & development in cartilage.</p>http://www.biomedcentral.com/1471-2474/13/130Kashin-beck diseaseMycotoxinsMicroarray |
| spellingShingle | Zhang Feng Guo Xiong Wang Weizhuo Wu Shixun Ma Weijuan Yan Hua Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease BMC Musculoskeletal Disorders Kashin-beck disease Mycotoxins Microarray |
| title | Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease |
| title_full | Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease |
| title_fullStr | Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease |
| title_full_unstemmed | Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease |
| title_short | Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease |
| title_sort | expression profile analysis of mycotoxin related genes in cartilage with endemic osteochondropathy kashin beck disease |
| topic | Kashin-beck disease Mycotoxins Microarray |
| url | http://www.biomedcentral.com/1471-2474/13/130 |
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