Chaga (), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B)
Background: Inonotus obliquus , also known as Chaga, is a parasitic fungus growing on birches and used in traditional medicine (especially by Khanty people) to treat various health problems. In this study, we aimed to quantify the 3 metabolites frequently cited in literature, that is, betulin, betul...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2018-09-01
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| Series: | Integrative Cancer Therapies |
| Online Access: | https://doi.org/10.1177/1534735418757912 |
| _version_ | 1819031644853501952 |
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| author | Antoine Géry PharmD Christelle Dubreule MSc Véronique André PharmD, PhD Jean-Philippe Rioult PharmD, PhD Valérie Bouchart PhD Natacha Heutte PhD Philippe Eldin de Pécoulas PharmD, PhD Tetyana Krivomaz PhD David Garon PharmD, PhD |
| author_facet | Antoine Géry PharmD Christelle Dubreule MSc Véronique André PharmD, PhD Jean-Philippe Rioult PharmD, PhD Valérie Bouchart PhD Natacha Heutte PhD Philippe Eldin de Pécoulas PharmD, PhD Tetyana Krivomaz PhD David Garon PharmD, PhD |
| author_sort | Antoine Géry PharmD |
| collection | DOAJ |
| description | Background: Inonotus obliquus , also known as Chaga, is a parasitic fungus growing on birches and used in traditional medicine (especially by Khanty people) to treat various health problems. In this study, we aimed to quantify the 3 metabolites frequently cited in literature, that is, betulin, betulinic acid, and inotodiol in the Chaga recently discovered in forests located in Normandy (France), and to compare their concentrations with Ukrainian and Canadian Chaga. This study also explores the cytotoxicity of the French Chaga against cancer-derived cells and transformed cells. Methods: A quantification method by HPLC-MS-MS (high-performance liquid chromatography–tandem mass spectrometry) of betulin, betulinic acid, and inotodiol was developed to study the French Chaga and compare the concentration of these metabolites with extracts provided from Chaga growing in Canada and Ukraine. This method was also used to identify and quantify those 3 compounds in other traditional preparations of Chaga (aqueous extract, infusion, and decoction). Among these preparations, the aqueous extract that contains betulin, betulinic acid, and inotodiol was chosen to evaluate and compare its cytotoxic activity toward human lung adenocarcinoma cells (A549 line) and human bronchial epithelial cells (BEAS-2B line). Results: French Chaga contains betulin and betulinic acid at higher levels than in other Chaga, whereas the concentration of inotodiol is greater in the Canadian Chaga. Moreover, the results highlighted a cytotoxic activity of the Chaga’s aqueous extract after 48 and 72 hours of exposure with a higher effect on cancer-derived cells A549 than on normal transformed cells BEAS-2B ( P = 0.025 after 48 hours of exposure and P = 0.004 after 72 hours of exposure). |
| first_indexed | 2024-12-21T06:49:20Z |
| format | Article |
| id | doaj.art-2c656880d0044984b63f2c65a31dbb87 |
| institution | Directory Open Access Journal |
| issn | 1552-695X |
| language | English |
| last_indexed | 2024-12-21T06:49:20Z |
| publishDate | 2018-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Integrative Cancer Therapies |
| spelling | doaj.art-2c656880d0044984b63f2c65a31dbb872022-12-21T19:12:30ZengSAGE PublishingIntegrative Cancer Therapies1552-695X2018-09-011710.1177/1534735418757912Chaga (), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B)Antoine Géry PharmD0Christelle Dubreule MSc1Véronique André PharmD, PhD2Jean-Philippe Rioult PharmD, PhD3Valérie Bouchart PhD4Natacha Heutte PhD5Philippe Eldin de Pécoulas PharmD, PhD6Tetyana Krivomaz PhD7David Garon PharmD, PhD8Normandie University, UNICAEN, Centre F. Baclesse, Caen, FranceLabéo Frank Duncombe, Saint-Contest, FranceNormandie University, UNICAEN, Centre F. Baclesse, Caen, FranceNormandie University, UNICAEN, Centre F. Baclesse, Caen, FranceLabéo Frank Duncombe, Saint-Contest, FranceNormandie University, UNIROUEN, FranceNormandie University, UNICAEN, Centre F. Baclesse, Caen, FranceNational University of Architecture and Construction, Kyiv, UkraineNormandie University, UNICAEN, Centre F. Baclesse, Caen, FranceBackground: Inonotus obliquus , also known as Chaga, is a parasitic fungus growing on birches and used in traditional medicine (especially by Khanty people) to treat various health problems. In this study, we aimed to quantify the 3 metabolites frequently cited in literature, that is, betulin, betulinic acid, and inotodiol in the Chaga recently discovered in forests located in Normandy (France), and to compare their concentrations with Ukrainian and Canadian Chaga. This study also explores the cytotoxicity of the French Chaga against cancer-derived cells and transformed cells. Methods: A quantification method by HPLC-MS-MS (high-performance liquid chromatography–tandem mass spectrometry) of betulin, betulinic acid, and inotodiol was developed to study the French Chaga and compare the concentration of these metabolites with extracts provided from Chaga growing in Canada and Ukraine. This method was also used to identify and quantify those 3 compounds in other traditional preparations of Chaga (aqueous extract, infusion, and decoction). Among these preparations, the aqueous extract that contains betulin, betulinic acid, and inotodiol was chosen to evaluate and compare its cytotoxic activity toward human lung adenocarcinoma cells (A549 line) and human bronchial epithelial cells (BEAS-2B line). Results: French Chaga contains betulin and betulinic acid at higher levels than in other Chaga, whereas the concentration of inotodiol is greater in the Canadian Chaga. Moreover, the results highlighted a cytotoxic activity of the Chaga’s aqueous extract after 48 and 72 hours of exposure with a higher effect on cancer-derived cells A549 than on normal transformed cells BEAS-2B ( P = 0.025 after 48 hours of exposure and P = 0.004 after 72 hours of exposure).https://doi.org/10.1177/1534735418757912 |
| spellingShingle | Antoine Géry PharmD Christelle Dubreule MSc Véronique André PharmD, PhD Jean-Philippe Rioult PharmD, PhD Valérie Bouchart PhD Natacha Heutte PhD Philippe Eldin de Pécoulas PharmD, PhD Tetyana Krivomaz PhD David Garon PharmD, PhD Chaga (), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) Integrative Cancer Therapies |
| title | Chaga (), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
| title_full | Chaga (), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
| title_fullStr | Chaga (), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
| title_full_unstemmed | Chaga (), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
| title_short | Chaga (), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
| title_sort | chaga a future potential medicinal fungus in oncology a chemical study and a comparison of the cytotoxicity against human lung adenocarcinoma cells a549 and human bronchial epithelial cells beas 2b |
| url | https://doi.org/10.1177/1534735418757912 |
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