Evaluation of gestational diabetes mellitus risk in South Indian women based on MTHFR (C677T) and FVL (G1691A) mutations
We aimed to scrutinize the extent to which single amino acid substitutions in the MTHFR and FVL genes affect the risk of GDM in pregnant women of South Indian descendant. This case-control study was implemented once the ethical approval has been obtained. Overall 237 women were recruited in this stu...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-05-01
|
| Series: | Frontiers in Pediatrics |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fped.2015.00034/full |
| _version_ | 1828779652097769472 |
|---|---|
| author | Imran Ali Khan Imran Ali Khan Imran Ali Khan Noor Ahmad Shaik Vasundara eKamineni Parveen eJahan Qurratulain eHasan Qurratulain eHasan Pragna eRao |
| author_facet | Imran Ali Khan Imran Ali Khan Imran Ali Khan Noor Ahmad Shaik Vasundara eKamineni Parveen eJahan Qurratulain eHasan Qurratulain eHasan Pragna eRao |
| author_sort | Imran Ali Khan |
| collection | DOAJ |
| description | We aimed to scrutinize the extent to which single amino acid substitutions in the MTHFR and FVL genes affect the risk of GDM in pregnant women of South Indian descendant. This case-control study was implemented once the ethical approval has been obtained. Overall 237 women were recruited in this study: 137 had been diagnosed with GDM and the remaining 100 women were used as normal controls or non-GDM. The diagnosis of GDM was confirmed with biochemical analysis i.e., GCT and OGTT tests. Five milliliters of peripheral blood was collected and used for biochemical and molecular analyses. DNA was isolated and genotyping for MTHFR (C677T) and FVL (G1691A) mutations was performed using PCR-RFLP. FVL (G1691A) locus was not polymorphic in the investigated sample. There was no significant difference in the allele and genotype frequencies of C677T polymorphism between GDM and non-GDM women (p=0.8892) |
| first_indexed | 2024-12-11T17:06:15Z |
| format | Article |
| id | doaj.art-2c66c7ee64824e6d9f69331688cffe08 |
| institution | Directory Open Access Journal |
| issn | 2296-2360 |
| language | English |
| last_indexed | 2024-12-11T17:06:15Z |
| publishDate | 2015-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pediatrics |
| spelling | doaj.art-2c66c7ee64824e6d9f69331688cffe082022-12-22T00:57:41ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602015-05-01310.3389/fped.2015.00034127993Evaluation of gestational diabetes mellitus risk in South Indian women based on MTHFR (C677T) and FVL (G1691A) mutationsImran Ali Khan0Imran Ali Khan1Imran Ali Khan2Noor Ahmad Shaik3Vasundara eKamineni4Parveen eJahan5Qurratulain eHasan6Qurratulain eHasan7Pragna eRao8Kamineni HospitalsVasavi Medical and Research CenterOsmania UniversityKing Abdulaziz UniversityKamineni HospitalsOsmania UniversityKamineni HospitalsVasavi Medical and Research CenterKasturba Medical College, Manipal UniversityWe aimed to scrutinize the extent to which single amino acid substitutions in the MTHFR and FVL genes affect the risk of GDM in pregnant women of South Indian descendant. This case-control study was implemented once the ethical approval has been obtained. Overall 237 women were recruited in this study: 137 had been diagnosed with GDM and the remaining 100 women were used as normal controls or non-GDM. The diagnosis of GDM was confirmed with biochemical analysis i.e., GCT and OGTT tests. Five milliliters of peripheral blood was collected and used for biochemical and molecular analyses. DNA was isolated and genotyping for MTHFR (C677T) and FVL (G1691A) mutations was performed using PCR-RFLP. FVL (G1691A) locus was not polymorphic in the investigated sample. There was no significant difference in the allele and genotype frequencies of C677T polymorphism between GDM and non-GDM women (p=0.8892)http://journal.frontiersin.org/Journal/10.3389/fped.2015.00034/fullMTHFRGestational diabetes mellitus (GDM)FVLC677TG1691A |
| spellingShingle | Imran Ali Khan Imran Ali Khan Imran Ali Khan Noor Ahmad Shaik Vasundara eKamineni Parveen eJahan Qurratulain eHasan Qurratulain eHasan Pragna eRao Evaluation of gestational diabetes mellitus risk in South Indian women based on MTHFR (C677T) and FVL (G1691A) mutations Frontiers in Pediatrics MTHFR Gestational diabetes mellitus (GDM) FVL C677T G1691A |
| title | Evaluation of gestational diabetes mellitus risk in South Indian women based on MTHFR (C677T) and FVL (G1691A) mutations |
| title_full | Evaluation of gestational diabetes mellitus risk in South Indian women based on MTHFR (C677T) and FVL (G1691A) mutations |
| title_fullStr | Evaluation of gestational diabetes mellitus risk in South Indian women based on MTHFR (C677T) and FVL (G1691A) mutations |
| title_full_unstemmed | Evaluation of gestational diabetes mellitus risk in South Indian women based on MTHFR (C677T) and FVL (G1691A) mutations |
| title_short | Evaluation of gestational diabetes mellitus risk in South Indian women based on MTHFR (C677T) and FVL (G1691A) mutations |
| title_sort | evaluation of gestational diabetes mellitus risk in south indian women based on mthfr c677t and fvl g1691a mutations |
| topic | MTHFR Gestational diabetes mellitus (GDM) FVL C677T G1691A |
| url | http://journal.frontiersin.org/Journal/10.3389/fped.2015.00034/full |
| work_keys_str_mv | AT imranalikhan evaluationofgestationaldiabetesmellitusriskinsouthindianwomenbasedonmthfrc677tandfvlg1691amutations AT imranalikhan evaluationofgestationaldiabetesmellitusriskinsouthindianwomenbasedonmthfrc677tandfvlg1691amutations AT imranalikhan evaluationofgestationaldiabetesmellitusriskinsouthindianwomenbasedonmthfrc677tandfvlg1691amutations AT noorahmadshaik evaluationofgestationaldiabetesmellitusriskinsouthindianwomenbasedonmthfrc677tandfvlg1691amutations AT vasundaraekamineni evaluationofgestationaldiabetesmellitusriskinsouthindianwomenbasedonmthfrc677tandfvlg1691amutations AT parveenejahan evaluationofgestationaldiabetesmellitusriskinsouthindianwomenbasedonmthfrc677tandfvlg1691amutations AT qurratulainehasan evaluationofgestationaldiabetesmellitusriskinsouthindianwomenbasedonmthfrc677tandfvlg1691amutations AT qurratulainehasan evaluationofgestationaldiabetesmellitusriskinsouthindianwomenbasedonmthfrc677tandfvlg1691amutations AT pragnaerao evaluationofgestationaldiabetesmellitusriskinsouthindianwomenbasedonmthfrc677tandfvlg1691amutations |