Potential of Zymomonas mobilis as an electricity producer in ethanol production
Abstract Background Microbial fuel cell (MFC) convokes microorganism to convert biomass into electricity. However, most well-known electrogenic strains cannot directly use glucose to produce valuable products. Zymomonas mobilis, a promising bacterium for ethanol production, owns special Entner–Doudo...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-03-01
|
| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-020-01672-5 |
| _version_ | 1818218843864039424 |
|---|---|
| author | Bo-Yu Geng Lian-Ying Cao Feng Li Hao Song Chen-Guang Liu Xin-Qing Zhao Feng-Wu Bai |
| author_facet | Bo-Yu Geng Lian-Ying Cao Feng Li Hao Song Chen-Guang Liu Xin-Qing Zhao Feng-Wu Bai |
| author_sort | Bo-Yu Geng |
| collection | DOAJ |
| description | Abstract Background Microbial fuel cell (MFC) convokes microorganism to convert biomass into electricity. However, most well-known electrogenic strains cannot directly use glucose to produce valuable products. Zymomonas mobilis, a promising bacterium for ethanol production, owns special Entner–Doudoroff pathway with less ATP and biomass produced and the low-energy coupling respiration, making Z. mobilis a potential exoelectrogen. Results A glucose-consuming MFC is constructed by inoculating Z. mobilis. The electricity with power density 2.0 mW/m2 is derived from the difference of oxidation–reduction potential (ORP) between anode and cathode chambers. Besides, two-type electricity generation is observed as glucose-independent process and glucose-dependent process. For the sake of enhancing MFC efficiency, extracellular and intracellular strategies are implemented. Biofilm removal and addition of c-type cytochrome benefit electricity performance and Tween 80 accelerates the electricity generation. Perturbation of cellular redox balance compromises the electricity output, indicating that redox homeostasis is the principal requirement to reach ideal voltage. Conclusion This study identifies potential feature of electricity activity for Z. mobilis and provides multiple strategies to enhance the electricity output. Therefore, additional electricity generation will benefit the techno-economic viability of the commercial bulk production for biochemicals or biofuels in an efficient and environmentally sustainable manner. |
| first_indexed | 2024-12-12T07:30:12Z |
| format | Article |
| id | doaj.art-2c67f96172144d5fa3940474f16877a8 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T07:30:12Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-2c67f96172144d5fa3940474f16877a82022-12-22T00:33:03ZengBMCBiotechnology for Biofuels1754-68342020-03-0113111110.1186/s13068-020-01672-5Potential of Zymomonas mobilis as an electricity producer in ethanol productionBo-Yu Geng0Lian-Ying Cao1Feng Li2Hao Song3Chen-Guang Liu4Xin-Qing Zhao5Feng-Wu Bai6State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences of Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences of Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityKey Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin UniversityKey Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences of Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences of Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences of Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityAbstract Background Microbial fuel cell (MFC) convokes microorganism to convert biomass into electricity. However, most well-known electrogenic strains cannot directly use glucose to produce valuable products. Zymomonas mobilis, a promising bacterium for ethanol production, owns special Entner–Doudoroff pathway with less ATP and biomass produced and the low-energy coupling respiration, making Z. mobilis a potential exoelectrogen. Results A glucose-consuming MFC is constructed by inoculating Z. mobilis. The electricity with power density 2.0 mW/m2 is derived from the difference of oxidation–reduction potential (ORP) between anode and cathode chambers. Besides, two-type electricity generation is observed as glucose-independent process and glucose-dependent process. For the sake of enhancing MFC efficiency, extracellular and intracellular strategies are implemented. Biofilm removal and addition of c-type cytochrome benefit electricity performance and Tween 80 accelerates the electricity generation. Perturbation of cellular redox balance compromises the electricity output, indicating that redox homeostasis is the principal requirement to reach ideal voltage. Conclusion This study identifies potential feature of electricity activity for Z. mobilis and provides multiple strategies to enhance the electricity output. Therefore, additional electricity generation will benefit the techno-economic viability of the commercial bulk production for biochemicals or biofuels in an efficient and environmentally sustainable manner.http://link.springer.com/article/10.1186/s13068-020-01672-5Zymomonas mobilisMicrobial fuel cell (MFC)BiofilmExtracellular electron transferRedox balance |
| spellingShingle | Bo-Yu Geng Lian-Ying Cao Feng Li Hao Song Chen-Guang Liu Xin-Qing Zhao Feng-Wu Bai Potential of Zymomonas mobilis as an electricity producer in ethanol production Biotechnology for Biofuels Zymomonas mobilis Microbial fuel cell (MFC) Biofilm Extracellular electron transfer Redox balance |
| title | Potential of Zymomonas mobilis as an electricity producer in ethanol production |
| title_full | Potential of Zymomonas mobilis as an electricity producer in ethanol production |
| title_fullStr | Potential of Zymomonas mobilis as an electricity producer in ethanol production |
| title_full_unstemmed | Potential of Zymomonas mobilis as an electricity producer in ethanol production |
| title_short | Potential of Zymomonas mobilis as an electricity producer in ethanol production |
| title_sort | potential of zymomonas mobilis as an electricity producer in ethanol production |
| topic | Zymomonas mobilis Microbial fuel cell (MFC) Biofilm Extracellular electron transfer Redox balance |
| url | http://link.springer.com/article/10.1186/s13068-020-01672-5 |
| work_keys_str_mv | AT boyugeng potentialofzymomonasmobilisasanelectricityproducerinethanolproduction AT lianyingcao potentialofzymomonasmobilisasanelectricityproducerinethanolproduction AT fengli potentialofzymomonasmobilisasanelectricityproducerinethanolproduction AT haosong potentialofzymomonasmobilisasanelectricityproducerinethanolproduction AT chenguangliu potentialofzymomonasmobilisasanelectricityproducerinethanolproduction AT xinqingzhao potentialofzymomonasmobilisasanelectricityproducerinethanolproduction AT fengwubai potentialofzymomonasmobilisasanelectricityproducerinethanolproduction |