Undercarboxylated osteocalcin is associated with vascular function in female older adults but does not influence vascular function in male rabbit carotid artery ex vivo.
<h4>Background</h4>There are conflicting reports on the association of undercarboxylated osteocalcin (ucOC) in cardiovascular disease development, including endothelial function and hypertension. We tested whether ucOC is related to blood pressure and endothelial function in older adults...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0242774 |
| _version_ | 1818737398306045952 |
|---|---|
| author | Alexander Tacey Cassandra Smith Mary N Woessner Paul Chubb Christopher Neil Gustavo Duque Alan Hayes Anthony Zulli Itamar Levinger |
| author_facet | Alexander Tacey Cassandra Smith Mary N Woessner Paul Chubb Christopher Neil Gustavo Duque Alan Hayes Anthony Zulli Itamar Levinger |
| author_sort | Alexander Tacey |
| collection | DOAJ |
| description | <h4>Background</h4>There are conflicting reports on the association of undercarboxylated osteocalcin (ucOC) in cardiovascular disease development, including endothelial function and hypertension. We tested whether ucOC is related to blood pressure and endothelial function in older adults, and if ucOC directly affects endothelial-mediated vasodilation in the carotid artery of rabbits.<h4>Methods</h4>In older adults, ucOC, blood pressure, pulse wave velocity (PWV) and brachial artery flow-mediated dilation (BAFMD) were measured (n = 38, 26 post-menopausal women and 12 men, mean age 73 ± 0.96). The vasoactivity of the carotid artery was assessed in male New Zealand White rabbits following a four-week normal or atherogenic diet using perfusion myography. An ucOC dose response curve (0.3-45 ng/ml) was generated following incubation of the arteries for 2-hours in either normal or high glucose conditions.<h4>Results</h4>ucOC levels were higher in normotensive older adults compared to those with stage 2 hypertension (p < 0.05), particularly in women (p < 0.01). In all participants, higher ucOC was associated with lower PWV (p < 0.05), but not BAFMD (p > 0.05). In rabbits, ucOC at any dose did not alter vasoactivity of the carotid artery, either following a normal or an atherogenic diet (p > 0.05).<h4>Conclusion</h4>Increased ucOC is associated with lower blood pressure and increased arterial stiffness, particularly in post-menopausal women. However, ucOC administration has no direct short-term effect on endothelial function in rabbit arteries. Future studies should explore whether treatment with ucOC, in vivo, has direct or indirect effects on blood vessel function. |
| first_indexed | 2024-12-18T00:52:25Z |
| format | Article |
| id | doaj.art-2c67ff119f2748d3b6681c8504855e62 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T00:52:25Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2c67ff119f2748d3b6681c8504855e622022-12-21T21:26:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011511e024277410.1371/journal.pone.0242774Undercarboxylated osteocalcin is associated with vascular function in female older adults but does not influence vascular function in male rabbit carotid artery ex vivo.Alexander TaceyCassandra SmithMary N WoessnerPaul ChubbChristopher NeilGustavo DuqueAlan HayesAnthony ZulliItamar Levinger<h4>Background</h4>There are conflicting reports on the association of undercarboxylated osteocalcin (ucOC) in cardiovascular disease development, including endothelial function and hypertension. We tested whether ucOC is related to blood pressure and endothelial function in older adults, and if ucOC directly affects endothelial-mediated vasodilation in the carotid artery of rabbits.<h4>Methods</h4>In older adults, ucOC, blood pressure, pulse wave velocity (PWV) and brachial artery flow-mediated dilation (BAFMD) were measured (n = 38, 26 post-menopausal women and 12 men, mean age 73 ± 0.96). The vasoactivity of the carotid artery was assessed in male New Zealand White rabbits following a four-week normal or atherogenic diet using perfusion myography. An ucOC dose response curve (0.3-45 ng/ml) was generated following incubation of the arteries for 2-hours in either normal or high glucose conditions.<h4>Results</h4>ucOC levels were higher in normotensive older adults compared to those with stage 2 hypertension (p < 0.05), particularly in women (p < 0.01). In all participants, higher ucOC was associated with lower PWV (p < 0.05), but not BAFMD (p > 0.05). In rabbits, ucOC at any dose did not alter vasoactivity of the carotid artery, either following a normal or an atherogenic diet (p > 0.05).<h4>Conclusion</h4>Increased ucOC is associated with lower blood pressure and increased arterial stiffness, particularly in post-menopausal women. However, ucOC administration has no direct short-term effect on endothelial function in rabbit arteries. Future studies should explore whether treatment with ucOC, in vivo, has direct or indirect effects on blood vessel function.https://doi.org/10.1371/journal.pone.0242774 |
| spellingShingle | Alexander Tacey Cassandra Smith Mary N Woessner Paul Chubb Christopher Neil Gustavo Duque Alan Hayes Anthony Zulli Itamar Levinger Undercarboxylated osteocalcin is associated with vascular function in female older adults but does not influence vascular function in male rabbit carotid artery ex vivo. PLoS ONE |
| title | Undercarboxylated osteocalcin is associated with vascular function in female older adults but does not influence vascular function in male rabbit carotid artery ex vivo. |
| title_full | Undercarboxylated osteocalcin is associated with vascular function in female older adults but does not influence vascular function in male rabbit carotid artery ex vivo. |
| title_fullStr | Undercarboxylated osteocalcin is associated with vascular function in female older adults but does not influence vascular function in male rabbit carotid artery ex vivo. |
| title_full_unstemmed | Undercarboxylated osteocalcin is associated with vascular function in female older adults but does not influence vascular function in male rabbit carotid artery ex vivo. |
| title_short | Undercarboxylated osteocalcin is associated with vascular function in female older adults but does not influence vascular function in male rabbit carotid artery ex vivo. |
| title_sort | undercarboxylated osteocalcin is associated with vascular function in female older adults but does not influence vascular function in male rabbit carotid artery ex vivo |
| url | https://doi.org/10.1371/journal.pone.0242774 |
| work_keys_str_mv | AT alexandertacey undercarboxylatedosteocalcinisassociatedwithvascularfunctioninfemaleolderadultsbutdoesnotinfluencevascularfunctioninmalerabbitcarotidarteryexvivo AT cassandrasmith undercarboxylatedosteocalcinisassociatedwithvascularfunctioninfemaleolderadultsbutdoesnotinfluencevascularfunctioninmalerabbitcarotidarteryexvivo AT marynwoessner undercarboxylatedosteocalcinisassociatedwithvascularfunctioninfemaleolderadultsbutdoesnotinfluencevascularfunctioninmalerabbitcarotidarteryexvivo AT paulchubb undercarboxylatedosteocalcinisassociatedwithvascularfunctioninfemaleolderadultsbutdoesnotinfluencevascularfunctioninmalerabbitcarotidarteryexvivo AT christopherneil undercarboxylatedosteocalcinisassociatedwithvascularfunctioninfemaleolderadultsbutdoesnotinfluencevascularfunctioninmalerabbitcarotidarteryexvivo AT gustavoduque undercarboxylatedosteocalcinisassociatedwithvascularfunctioninfemaleolderadultsbutdoesnotinfluencevascularfunctioninmalerabbitcarotidarteryexvivo AT alanhayes undercarboxylatedosteocalcinisassociatedwithvascularfunctioninfemaleolderadultsbutdoesnotinfluencevascularfunctioninmalerabbitcarotidarteryexvivo AT anthonyzulli undercarboxylatedosteocalcinisassociatedwithvascularfunctioninfemaleolderadultsbutdoesnotinfluencevascularfunctioninmalerabbitcarotidarteryexvivo AT itamarlevinger undercarboxylatedosteocalcinisassociatedwithvascularfunctioninfemaleolderadultsbutdoesnotinfluencevascularfunctioninmalerabbitcarotidarteryexvivo |