Transcript Regulation of the Recoded Archaeal α-<span style="font-variant: small-caps">l</span>-Fucosidase In Vivo
Genetic decoding is flexible, due to programmed deviation of the ribosomes from standard translational rules, globally termed “recoding”. In <i>Archaea</i>, recoding has been unequivocally determined only for termination codon readthrough events that regulate the incorporation of the unu...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/7/1861 |
| _version_ | 1797540008974876672 |
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| author | Federica De Lise Roberta Iacono Andrea Strazzulli Rosa Giglio Nicola Curci Luisa Maurelli Rosario Avino Antonio Carandente Stefano Caliro Alessandra Tortora Fabio Lorenzini Paola Di Donato Marco Moracci Beatrice Cobucci-Ponzano |
| author_facet | Federica De Lise Roberta Iacono Andrea Strazzulli Rosa Giglio Nicola Curci Luisa Maurelli Rosario Avino Antonio Carandente Stefano Caliro Alessandra Tortora Fabio Lorenzini Paola Di Donato Marco Moracci Beatrice Cobucci-Ponzano |
| author_sort | Federica De Lise |
| collection | DOAJ |
| description | Genetic decoding is flexible, due to programmed deviation of the ribosomes from standard translational rules, globally termed “recoding”. In <i>Archaea</i>, recoding has been unequivocally determined only for termination codon readthrough events that regulate the incorporation of the unusual amino acids selenocysteine and pyrrolysine, and for −1 programmed frameshifting that allow the expression of a fully functional α-<span style="font-variant: small-caps;">l</span>-fucosidase in the crenarchaeon <i>Saccharolobus solfataricus</i>, in which several functional interrupted genes have been identified. Increasing evidence suggests that the flexibility of the genetic code decoding could provide an evolutionary advantage in extreme conditions, therefore, the identification and study of interrupted genes in extremophilic Archaea could be important from an astrobiological point of view, providing new information on the origin and evolution of the genetic code and on the limits of life on Earth. In order to shed some light on the mechanism of programmed −1 frameshifting in Archaea, here we report, for the first time, on the analysis of the transcription of this recoded archaeal α-<span style="font-variant: small-caps;">l</span>-fucosidase and of its full-length mutant in different growth conditions in vivo. We found that only the wild type mRNA significantly increased in <i>S. solfataricus</i> after cold shock and in cells grown in minimal medium containing hydrolyzed xyloglucan as carbon source. Our results indicated that the increased level of <i>fucA</i> mRNA cannot be explained by transcript up-regulation alone. A different mechanism related to translation efficiency is discussed. |
| first_indexed | 2024-03-10T12:53:58Z |
| format | Article |
| id | doaj.art-2c6f2d6a1c4842fe88e443fe6a0f13b0 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T12:53:58Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2c6f2d6a1c4842fe88e443fe6a0f13b02023-11-21T12:02:39ZengMDPI AGMolecules1420-30492021-03-01267186110.3390/molecules26071861Transcript Regulation of the Recoded Archaeal α-<span style="font-variant: small-caps">l</span>-Fucosidase In VivoFederica De Lise0Roberta Iacono1Andrea Strazzulli2Rosa Giglio3Nicola Curci4Luisa Maurelli5Rosario Avino6Antonio Carandente7Stefano Caliro8Alessandra Tortora9Fabio Lorenzini10Paola Di Donato11Marco Moracci12Beatrice Cobucci-Ponzano13Institute of Biosciences and BioResources, National Research Council of Italy, Via P. Castellino 111, 80131 Naples, ItalyInstitute of Biosciences and BioResources, National Research Council of Italy, Via P. Castellino 111, 80131 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, Complesso Universitario Di Monte S. Angelo, Via Cupa Nuova Cinthia 21, 80126 Naples, ItalyInstitute of Biosciences and BioResources, National Research Council of Italy, Via P. Castellino 111, 80131 Naples, ItalyInstitute of Biosciences and BioResources, National Research Council of Italy, Via P. Castellino 111, 80131 Naples, ItalyInstitute of Biosciences and BioResources, National Research Council of Italy, Via P. Castellino 111, 80131 Naples, ItalyNational Institute of Geophysics and Volcanology, Via Diocleziano, 328, 80125 Naples, ItalyNational Institute of Geophysics and Volcanology, Via Diocleziano, 328, 80125 Naples, ItalyNational Institute of Geophysics and Volcanology, Via Diocleziano, 328, 80125 Naples, ItalyKayser Italia Srl., Via di Popogna, 501, 57128 Livorno, ItalyKayser Italia Srl., Via di Popogna, 501, 57128 Livorno, ItalyInstitute of Biomolecular Chemistry, National Research Council of Italy, Via Campi Flegrei 34, 80078 Pozzuoli, ItalyInstitute of Biosciences and BioResources, National Research Council of Italy, Via P. Castellino 111, 80131 Naples, ItalyInstitute of Biosciences and BioResources, National Research Council of Italy, Via P. Castellino 111, 80131 Naples, ItalyGenetic decoding is flexible, due to programmed deviation of the ribosomes from standard translational rules, globally termed “recoding”. In <i>Archaea</i>, recoding has been unequivocally determined only for termination codon readthrough events that regulate the incorporation of the unusual amino acids selenocysteine and pyrrolysine, and for −1 programmed frameshifting that allow the expression of a fully functional α-<span style="font-variant: small-caps;">l</span>-fucosidase in the crenarchaeon <i>Saccharolobus solfataricus</i>, in which several functional interrupted genes have been identified. Increasing evidence suggests that the flexibility of the genetic code decoding could provide an evolutionary advantage in extreme conditions, therefore, the identification and study of interrupted genes in extremophilic Archaea could be important from an astrobiological point of view, providing new information on the origin and evolution of the genetic code and on the limits of life on Earth. In order to shed some light on the mechanism of programmed −1 frameshifting in Archaea, here we report, for the first time, on the analysis of the transcription of this recoded archaeal α-<span style="font-variant: small-caps;">l</span>-fucosidase and of its full-length mutant in different growth conditions in vivo. We found that only the wild type mRNA significantly increased in <i>S. solfataricus</i> after cold shock and in cells grown in minimal medium containing hydrolyzed xyloglucan as carbon source. Our results indicated that the increased level of <i>fucA</i> mRNA cannot be explained by transcript up-regulation alone. A different mechanism related to translation efficiency is discussed.https://www.mdpi.com/1420-3049/26/7/1861<i>Archaea</i>extremophilesrecodingprogrammed frameshiftinglimits of life |
| spellingShingle | Federica De Lise Roberta Iacono Andrea Strazzulli Rosa Giglio Nicola Curci Luisa Maurelli Rosario Avino Antonio Carandente Stefano Caliro Alessandra Tortora Fabio Lorenzini Paola Di Donato Marco Moracci Beatrice Cobucci-Ponzano Transcript Regulation of the Recoded Archaeal α-<span style="font-variant: small-caps">l</span>-Fucosidase In Vivo Molecules <i>Archaea</i> extremophiles recoding programmed frameshifting limits of life |
| title | Transcript Regulation of the Recoded Archaeal α-<span style="font-variant: small-caps">l</span>-Fucosidase In Vivo |
| title_full | Transcript Regulation of the Recoded Archaeal α-<span style="font-variant: small-caps">l</span>-Fucosidase In Vivo |
| title_fullStr | Transcript Regulation of the Recoded Archaeal α-<span style="font-variant: small-caps">l</span>-Fucosidase In Vivo |
| title_full_unstemmed | Transcript Regulation of the Recoded Archaeal α-<span style="font-variant: small-caps">l</span>-Fucosidase In Vivo |
| title_short | Transcript Regulation of the Recoded Archaeal α-<span style="font-variant: small-caps">l</span>-Fucosidase In Vivo |
| title_sort | transcript regulation of the recoded archaeal α span style font variant small caps l span fucosidase in vivo |
| topic | <i>Archaea</i> extremophiles recoding programmed frameshifting limits of life |
| url | https://www.mdpi.com/1420-3049/26/7/1861 |
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