Modulation of Phenylalanine and Tyrosine Metabolism in HIV-1 Infected Patients with Neurocognitive Impairment: Results from a Clinical Trial
To investigate the effects of oral bacteriotherapy on intestinal phenylalanine and tyrosine metabolism, in this longitudinal, double-arm trial, 15 virally suppressed HIV+ individuals underwent blood and fecal sample collection at baseline and after 6 months of oral bacteriotherapy. A baseline fecal...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/10/7/274 |
| _version_ | 1827713677003849728 |
|---|---|
| author | Giuseppe P. Innocenti Letizia Santinelli Luca Laghi Cristian Borrazzo Claudia Pinacchio Mariangela Fratino Luigi Celani Eugenio N. Cavallari Carolina Scagnolari Federica Frasca Guido Antonelli Claudio M. Mastroianni Gabriella d’Ettorre Giancarlo Ceccarelli |
| author_facet | Giuseppe P. Innocenti Letizia Santinelli Luca Laghi Cristian Borrazzo Claudia Pinacchio Mariangela Fratino Luigi Celani Eugenio N. Cavallari Carolina Scagnolari Federica Frasca Guido Antonelli Claudio M. Mastroianni Gabriella d’Ettorre Giancarlo Ceccarelli |
| author_sort | Giuseppe P. Innocenti |
| collection | DOAJ |
| description | To investigate the effects of oral bacteriotherapy on intestinal phenylalanine and tyrosine metabolism, in this longitudinal, double-arm trial, 15 virally suppressed HIV+ individuals underwent blood and fecal sample collection at baseline and after 6 months of oral bacteriotherapy. A baseline fecal sample was collected from 15 healthy individuals and served as control group for the baseline levels of fecal phenylalanine and tyrosine. CD4 and CD8 immune activation (CD38<sup>+</sup>) was evaluated by flow cytometry. Amino acid evaluation on fecal samples was conducted by Proton Nuclear Magnetic Resonance. Results showed that HIV+ participants displayed higher baseline phenylalanine/tyrosine ratio values than healthy volunteers. A significand reduction in phenylalanine/tyrosine ratio and peripheral CD4<sup>+</sup> CD38<sup>+</sup> activation was observed at the end of oral bacteriotherapy. In conclusion, probiotics beneficially affect the immune activation of HIV+ individuals. Therefore, the restoration of intestinal amino acid metabolism could represent the mechanisms through which probiotics exert these desirable effects. |
| first_indexed | 2024-03-10T18:41:18Z |
| format | Article |
| id | doaj.art-2c70b9ec90634a6b88f5f33a1d7bb09f |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T18:41:18Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-2c70b9ec90634a6b88f5f33a1d7bb09f2023-11-20T05:49:43ZengMDPI AGMetabolites2218-19892020-07-0110727410.3390/metabo10070274Modulation of Phenylalanine and Tyrosine Metabolism in HIV-1 Infected Patients with Neurocognitive Impairment: Results from a Clinical TrialGiuseppe P. Innocenti0Letizia Santinelli1Luca Laghi2Cristian Borrazzo3Claudia Pinacchio4Mariangela Fratino5Luigi Celani6Eugenio N. Cavallari7Carolina Scagnolari8Federica Frasca9Guido Antonelli10Claudio M. Mastroianni11Gabriella d’Ettorre12Giancarlo Ceccarelli13Department of Public Health and Infectious Diseases, Sapienza, University of Rome, viale del Policlinico 155, 00161 Rome, ItalyDepartment of Public Health and Infectious Diseases, Sapienza, University of Rome, viale del Policlinico 155, 00161 Rome, ItalyDepartment of Agro-Food Science and Technology, University of Bologna, Viale Fanin 46, 40127 Bologna, ItalyDepartment of Public Health and Infectious Diseases, Sapienza, University of Rome, viale del Policlinico 155, 00161 Rome, ItalyDepartment of Public Health and Infectious Diseases, Sapienza, University of Rome, viale del Policlinico 155, 00161 Rome, ItalyDepartment of Neurology Sapienza, University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Public Health and Infectious Diseases, Sapienza, University of Rome, viale del Policlinico 155, 00161 Rome, ItalyDepartment of Public Health and Infectious Diseases, Sapienza, University of Rome, viale del Policlinico 155, 00161 Rome, ItalyLaboratory of Virology, Department of Molecular Medicine, Affiliated to Istituto Pasteur Italia-Cenci Bolognetti Foundation, Sapienza, University of Rome, 00185 Rome, ItalyLaboratory of Virology, Department of Molecular Medicine, Affiliated to Istituto Pasteur Italia-Cenci Bolognetti Foundation, Sapienza, University of Rome, 00185 Rome, ItalyLaboratory of Virology, Department of Molecular Medicine, Affiliated to Istituto Pasteur Italia-Cenci Bolognetti Foundation, Sapienza, University of Rome, 00185 Rome, ItalyDepartment of Public Health and Infectious Diseases, Sapienza, University of Rome, viale del Policlinico 155, 00161 Rome, ItalyDepartment of Public Health and Infectious Diseases, Sapienza, University of Rome, viale del Policlinico 155, 00161 Rome, ItalyDepartment of Public Health and Infectious Diseases, Sapienza, University of Rome, viale del Policlinico 155, 00161 Rome, ItalyTo investigate the effects of oral bacteriotherapy on intestinal phenylalanine and tyrosine metabolism, in this longitudinal, double-arm trial, 15 virally suppressed HIV+ individuals underwent blood and fecal sample collection at baseline and after 6 months of oral bacteriotherapy. A baseline fecal sample was collected from 15 healthy individuals and served as control group for the baseline levels of fecal phenylalanine and tyrosine. CD4 and CD8 immune activation (CD38<sup>+</sup>) was evaluated by flow cytometry. Amino acid evaluation on fecal samples was conducted by Proton Nuclear Magnetic Resonance. Results showed that HIV+ participants displayed higher baseline phenylalanine/tyrosine ratio values than healthy volunteers. A significand reduction in phenylalanine/tyrosine ratio and peripheral CD4<sup>+</sup> CD38<sup>+</sup> activation was observed at the end of oral bacteriotherapy. In conclusion, probiotics beneficially affect the immune activation of HIV+ individuals. Therefore, the restoration of intestinal amino acid metabolism could represent the mechanisms through which probiotics exert these desirable effects.https://www.mdpi.com/2218-1989/10/7/274HIVprobioticsphenylalaninetyrosinemetabolisminflammation |
| spellingShingle | Giuseppe P. Innocenti Letizia Santinelli Luca Laghi Cristian Borrazzo Claudia Pinacchio Mariangela Fratino Luigi Celani Eugenio N. Cavallari Carolina Scagnolari Federica Frasca Guido Antonelli Claudio M. Mastroianni Gabriella d’Ettorre Giancarlo Ceccarelli Modulation of Phenylalanine and Tyrosine Metabolism in HIV-1 Infected Patients with Neurocognitive Impairment: Results from a Clinical Trial Metabolites HIV probiotics phenylalanine tyrosine metabolism inflammation |
| title | Modulation of Phenylalanine and Tyrosine Metabolism in HIV-1 Infected Patients with Neurocognitive Impairment: Results from a Clinical Trial |
| title_full | Modulation of Phenylalanine and Tyrosine Metabolism in HIV-1 Infected Patients with Neurocognitive Impairment: Results from a Clinical Trial |
| title_fullStr | Modulation of Phenylalanine and Tyrosine Metabolism in HIV-1 Infected Patients with Neurocognitive Impairment: Results from a Clinical Trial |
| title_full_unstemmed | Modulation of Phenylalanine and Tyrosine Metabolism in HIV-1 Infected Patients with Neurocognitive Impairment: Results from a Clinical Trial |
| title_short | Modulation of Phenylalanine and Tyrosine Metabolism in HIV-1 Infected Patients with Neurocognitive Impairment: Results from a Clinical Trial |
| title_sort | modulation of phenylalanine and tyrosine metabolism in hiv 1 infected patients with neurocognitive impairment results from a clinical trial |
| topic | HIV probiotics phenylalanine tyrosine metabolism inflammation |
| url | https://www.mdpi.com/2218-1989/10/7/274 |
| work_keys_str_mv | AT giuseppepinnocenti modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT letiziasantinelli modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT lucalaghi modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT cristianborrazzo modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT claudiapinacchio modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT mariangelafratino modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT luigicelani modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT eugenioncavallari modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT carolinascagnolari modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT federicafrasca modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT guidoantonelli modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT claudiommastroianni modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT gabrielladettorre modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial AT giancarloceccarelli modulationofphenylalanineandtyrosinemetabolisminhiv1infectedpatientswithneurocognitiveimpairmentresultsfromaclinicaltrial |