Antihyperlipidemic Effect of Different Fractions Obtained from Teucrium polium Hydroalcoholic Extract in Rats
Background: This study was aimed to screen the antihyperlipidemic effect of different fractions of Teucrium polium to obtain the most efficient herbal fraction for isolation of bioactive constituents responsible for hypolipidemic activity. Methods: Chloroform, butanol, and aqueous fractions were obt...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | International Journal of Preventive Medicine |
| Subjects: | |
| Online Access: | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2018;volume=9;issue=1;spage=30;epage=30;aulast=Safaeian |
| _version_ | 1818546570200612864 |
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| author | Leila Safaeian Mustafa Ghanadian Zahra Shafiee-Moghadam |
| author_facet | Leila Safaeian Mustafa Ghanadian Zahra Shafiee-Moghadam |
| author_sort | Leila Safaeian |
| collection | DOAJ |
| description | Background: This study was aimed to screen the antihyperlipidemic effect of different fractions of Teucrium polium to obtain the most efficient herbal fraction for isolation of bioactive constituents responsible for hypolipidemic activity. Methods: Chloroform, butanol, and aqueous fractions were obtained from hydroalcoholic extract of T. polium aerial parts using partitioning process. To induce hyperlipidemia, dexamethasone (Dex) was injected 10 mg/kg/day (s.c.) for 8 days. In the test groups, animals received 50, 100 and 150 mg/kg of T. polium hydroalcoholic extract and different fractions orally simultaneously with Dex. Serum lipid profile and hepatic marker enzymes were evaluated using biochemical kits. Results: All treatments, especially chloroform and aqueous fractions, reversed serum lipid markers in hyperlipidemic rats. Maximum reduction in triglyceride (60.2%, P < 0.001) and maximum elevation in high-density lipoprotein (HDL) (35.0%, P < 0.01) was observed for chloroform fraction. Maximum cholesterol-lowering effect (29.0%, P < 0.001) and maximum reduction in low-density lipoprotein were found for hydroalcoholic extract (72.9%, P < 0.001). Aqueous fraction improved all lipid markers at the highest dose. Butanol fraction decreased triglyceride at the lowest dose (43.9%, P < 0.001) and increased HDL (33%, P < 0.05) at the highest dose. There was a significant increase in alanine aminotransferase and aspartate aminotransferase levels in all tested groups compared to normal group (P < 0.001). Conclusions: This study showed strong antihyperlipidemic effect of various fractions derived from hydroalcoholic extract of T. polium. Chloroform and aqueous fractions may be worthy candidates for isolation of bioactive hypolipidemic constituents. However, possible hepatotoxicity should be considered for clinical application. |
| first_indexed | 2024-12-12T07:54:59Z |
| format | Article |
| id | doaj.art-2c70c07f2dd149979c60c1f67bc1f425 |
| institution | Directory Open Access Journal |
| issn | 2008-7802 2008-8213 |
| language | English |
| last_indexed | 2024-12-12T07:54:59Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Preventive Medicine |
| spelling | doaj.art-2c70c07f2dd149979c60c1f67bc1f4252022-12-22T00:32:20ZengWolters Kluwer Medknow PublicationsInternational Journal of Preventive Medicine2008-78022008-82132018-01-0191303010.4103/ijpvm.IJPVM_100_17Antihyperlipidemic Effect of Different Fractions Obtained from Teucrium polium Hydroalcoholic Extract in RatsLeila SafaeianMustafa GhanadianZahra Shafiee-MoghadamBackground: This study was aimed to screen the antihyperlipidemic effect of different fractions of Teucrium polium to obtain the most efficient herbal fraction for isolation of bioactive constituents responsible for hypolipidemic activity. Methods: Chloroform, butanol, and aqueous fractions were obtained from hydroalcoholic extract of T. polium aerial parts using partitioning process. To induce hyperlipidemia, dexamethasone (Dex) was injected 10 mg/kg/day (s.c.) for 8 days. In the test groups, animals received 50, 100 and 150 mg/kg of T. polium hydroalcoholic extract and different fractions orally simultaneously with Dex. Serum lipid profile and hepatic marker enzymes were evaluated using biochemical kits. Results: All treatments, especially chloroform and aqueous fractions, reversed serum lipid markers in hyperlipidemic rats. Maximum reduction in triglyceride (60.2%, P < 0.001) and maximum elevation in high-density lipoprotein (HDL) (35.0%, P < 0.01) was observed for chloroform fraction. Maximum cholesterol-lowering effect (29.0%, P < 0.001) and maximum reduction in low-density lipoprotein were found for hydroalcoholic extract (72.9%, P < 0.001). Aqueous fraction improved all lipid markers at the highest dose. Butanol fraction decreased triglyceride at the lowest dose (43.9%, P < 0.001) and increased HDL (33%, P < 0.05) at the highest dose. There was a significant increase in alanine aminotransferase and aspartate aminotransferase levels in all tested groups compared to normal group (P < 0.001). Conclusions: This study showed strong antihyperlipidemic effect of various fractions derived from hydroalcoholic extract of T. polium. Chloroform and aqueous fractions may be worthy candidates for isolation of bioactive hypolipidemic constituents. However, possible hepatotoxicity should be considered for clinical application.http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2018;volume=9;issue=1;spage=30;epage=30;aulast=SafaeianDexamethasonehepatotoxicityhyperlipidemiaTeucrium polium |
| spellingShingle | Leila Safaeian Mustafa Ghanadian Zahra Shafiee-Moghadam Antihyperlipidemic Effect of Different Fractions Obtained from Teucrium polium Hydroalcoholic Extract in Rats International Journal of Preventive Medicine Dexamethasone hepatotoxicity hyperlipidemia Teucrium polium |
| title | Antihyperlipidemic Effect of Different Fractions Obtained from Teucrium polium Hydroalcoholic Extract in Rats |
| title_full | Antihyperlipidemic Effect of Different Fractions Obtained from Teucrium polium Hydroalcoholic Extract in Rats |
| title_fullStr | Antihyperlipidemic Effect of Different Fractions Obtained from Teucrium polium Hydroalcoholic Extract in Rats |
| title_full_unstemmed | Antihyperlipidemic Effect of Different Fractions Obtained from Teucrium polium Hydroalcoholic Extract in Rats |
| title_short | Antihyperlipidemic Effect of Different Fractions Obtained from Teucrium polium Hydroalcoholic Extract in Rats |
| title_sort | antihyperlipidemic effect of different fractions obtained from teucrium polium hydroalcoholic extract in rats |
| topic | Dexamethasone hepatotoxicity hyperlipidemia Teucrium polium |
| url | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2018;volume=9;issue=1;spage=30;epage=30;aulast=Safaeian |
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