Natural type 3/type 2 intertypic vaccine-related poliovirus recombinants with the first crossover sites within the VP1 capsid coding region.
BACKGROUND: Ten uncommon natural type 3/type 2 intertypic poliovirus recombinants were isolated from stool specimens from nine acute flaccid paralysis case patients and one healthy vaccinee in China from 2001 to 2008. PRINCIPAL FINDINGS: Complete genomic sequences revealed their vaccine-related geno...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3006203?pdf=render |
| _version_ | 1818438008192368640 |
|---|---|
| author | Yong Zhang Shuangli Zhu Dongmei Yan Guiyan Liu Ruyin Bai Dongyan Wang Li Chen Hui Zhu Hongqiu An Olen Kew Wenbo Xu |
| author_facet | Yong Zhang Shuangli Zhu Dongmei Yan Guiyan Liu Ruyin Bai Dongyan Wang Li Chen Hui Zhu Hongqiu An Olen Kew Wenbo Xu |
| author_sort | Yong Zhang |
| collection | DOAJ |
| description | BACKGROUND: Ten uncommon natural type 3/type 2 intertypic poliovirus recombinants were isolated from stool specimens from nine acute flaccid paralysis case patients and one healthy vaccinee in China from 2001 to 2008. PRINCIPAL FINDINGS: Complete genomic sequences revealed their vaccine-related genomic features and showed that their first crossover sites were randomly distributed in the 3' end of the VP1 coding region. The length of donor Sabin 2 sequences ranged from 55 to 136 nucleotides, which is the longest donor sequence reported in the literature for this type of poliovirus recombination. The recombination resulted in the introduction of Sabin 2 neutralizing antigenic site 3a (NAg3a) into a Sabin 3 genomic background in the VP1 coding region, which may have been altered by some of the type 3-specific antigenic properties, but had not acquired any type 2-specific characterizations. NAg3a of the Sabin 3 strain seems atypical; other wild-type poliovirus isolates that have circulated in recent years have sequences of NAg3a more like the Sabin 2 strain. CONCLUSIONS: 10 natural type 3/type 2 intertypic VP1 capsid-recombinant polioviruses, in which the first crossover sites were found to be in the VP1 coding region, were isolated and characterized. In spite of the complete replacement of NAg3a by type 2-specific amino acids, the serotypes of the recombinants were not altered, and they were totally neutralized by polyclonal type 3 antisera but not at all by type 2 antisera. It is possible that recent type 3 wild poliovirus isolates may be a recombinant having NAg3a sequences derived from another strain during between 1967 and 1980, and the type 3/type 2 recombination events in the 3' end of the VP1 coding region may result in a higher fitness. |
| first_indexed | 2024-12-14T17:33:44Z |
| format | Article |
| id | doaj.art-2c7325726efb4d81a3e25e86118f4b60 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T17:33:44Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2c7325726efb4d81a3e25e86118f4b602022-12-21T22:53:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-01512e1530010.1371/journal.pone.0015300Natural type 3/type 2 intertypic vaccine-related poliovirus recombinants with the first crossover sites within the VP1 capsid coding region.Yong ZhangShuangli ZhuDongmei YanGuiyan LiuRuyin BaiDongyan WangLi ChenHui ZhuHongqiu AnOlen KewWenbo XuBACKGROUND: Ten uncommon natural type 3/type 2 intertypic poliovirus recombinants were isolated from stool specimens from nine acute flaccid paralysis case patients and one healthy vaccinee in China from 2001 to 2008. PRINCIPAL FINDINGS: Complete genomic sequences revealed their vaccine-related genomic features and showed that their first crossover sites were randomly distributed in the 3' end of the VP1 coding region. The length of donor Sabin 2 sequences ranged from 55 to 136 nucleotides, which is the longest donor sequence reported in the literature for this type of poliovirus recombination. The recombination resulted in the introduction of Sabin 2 neutralizing antigenic site 3a (NAg3a) into a Sabin 3 genomic background in the VP1 coding region, which may have been altered by some of the type 3-specific antigenic properties, but had not acquired any type 2-specific characterizations. NAg3a of the Sabin 3 strain seems atypical; other wild-type poliovirus isolates that have circulated in recent years have sequences of NAg3a more like the Sabin 2 strain. CONCLUSIONS: 10 natural type 3/type 2 intertypic VP1 capsid-recombinant polioviruses, in which the first crossover sites were found to be in the VP1 coding region, were isolated and characterized. In spite of the complete replacement of NAg3a by type 2-specific amino acids, the serotypes of the recombinants were not altered, and they were totally neutralized by polyclonal type 3 antisera but not at all by type 2 antisera. It is possible that recent type 3 wild poliovirus isolates may be a recombinant having NAg3a sequences derived from another strain during between 1967 and 1980, and the type 3/type 2 recombination events in the 3' end of the VP1 coding region may result in a higher fitness.http://europepmc.org/articles/PMC3006203?pdf=render |
| spellingShingle | Yong Zhang Shuangli Zhu Dongmei Yan Guiyan Liu Ruyin Bai Dongyan Wang Li Chen Hui Zhu Hongqiu An Olen Kew Wenbo Xu Natural type 3/type 2 intertypic vaccine-related poliovirus recombinants with the first crossover sites within the VP1 capsid coding region. PLoS ONE |
| title | Natural type 3/type 2 intertypic vaccine-related poliovirus recombinants with the first crossover sites within the VP1 capsid coding region. |
| title_full | Natural type 3/type 2 intertypic vaccine-related poliovirus recombinants with the first crossover sites within the VP1 capsid coding region. |
| title_fullStr | Natural type 3/type 2 intertypic vaccine-related poliovirus recombinants with the first crossover sites within the VP1 capsid coding region. |
| title_full_unstemmed | Natural type 3/type 2 intertypic vaccine-related poliovirus recombinants with the first crossover sites within the VP1 capsid coding region. |
| title_short | Natural type 3/type 2 intertypic vaccine-related poliovirus recombinants with the first crossover sites within the VP1 capsid coding region. |
| title_sort | natural type 3 type 2 intertypic vaccine related poliovirus recombinants with the first crossover sites within the vp1 capsid coding region |
| url | http://europepmc.org/articles/PMC3006203?pdf=render |
| work_keys_str_mv | AT yongzhang naturaltype3type2intertypicvaccinerelatedpoliovirusrecombinantswiththefirstcrossoversiteswithinthevp1capsidcodingregion AT shuanglizhu naturaltype3type2intertypicvaccinerelatedpoliovirusrecombinantswiththefirstcrossoversiteswithinthevp1capsidcodingregion AT dongmeiyan naturaltype3type2intertypicvaccinerelatedpoliovirusrecombinantswiththefirstcrossoversiteswithinthevp1capsidcodingregion AT guiyanliu naturaltype3type2intertypicvaccinerelatedpoliovirusrecombinantswiththefirstcrossoversiteswithinthevp1capsidcodingregion AT ruyinbai naturaltype3type2intertypicvaccinerelatedpoliovirusrecombinantswiththefirstcrossoversiteswithinthevp1capsidcodingregion AT dongyanwang naturaltype3type2intertypicvaccinerelatedpoliovirusrecombinantswiththefirstcrossoversiteswithinthevp1capsidcodingregion AT lichen naturaltype3type2intertypicvaccinerelatedpoliovirusrecombinantswiththefirstcrossoversiteswithinthevp1capsidcodingregion AT huizhu naturaltype3type2intertypicvaccinerelatedpoliovirusrecombinantswiththefirstcrossoversiteswithinthevp1capsidcodingregion AT hongqiuan naturaltype3type2intertypicvaccinerelatedpoliovirusrecombinantswiththefirstcrossoversiteswithinthevp1capsidcodingregion AT olenkew naturaltype3type2intertypicvaccinerelatedpoliovirusrecombinantswiththefirstcrossoversiteswithinthevp1capsidcodingregion AT wenboxu naturaltype3type2intertypicvaccinerelatedpoliovirusrecombinantswiththefirstcrossoversiteswithinthevp1capsidcodingregion |