Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart
AimsAlthough voltage-sensitive dye di-4-ANEPPS is a common tool for mapping cardiac electrical activity, reported effects on electrophysiological parameters are rather. The main goals of the study were to reveal effects of the dye on rabbit isolated heart and to verify, whether rabbit isolated heart...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.667065/full |
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| author | Marina Ronzhina Tibor Stracina Lubica Lacinova Katarina Ondacova Michaela Pavlovicova Lucie Marsanova Radovan Smisek Oto Janousek Katerina Fialova Jana Kolarova Marie Novakova Marie Novakova Ivo Provaznik |
| author_facet | Marina Ronzhina Tibor Stracina Lubica Lacinova Katarina Ondacova Michaela Pavlovicova Lucie Marsanova Radovan Smisek Oto Janousek Katerina Fialova Jana Kolarova Marie Novakova Marie Novakova Ivo Provaznik |
| author_sort | Marina Ronzhina |
| collection | DOAJ |
| description | AimsAlthough voltage-sensitive dye di-4-ANEPPS is a common tool for mapping cardiac electrical activity, reported effects on electrophysiological parameters are rather. The main goals of the study were to reveal effects of the dye on rabbit isolated heart and to verify, whether rabbit isolated heart stained with di-4-ANEPPS is a suitable tool for myocardial ischemia investigation.Methods and ResultsStudy involved experiments on stained (n = 9) and non-stained (n = 11) Langendorff perfused rabbit isolated hearts. Electrophysiological effects of the dye were evaluated by analysis of various electrogram (EG) parameters using common paired and unpaired statistical tests. It was shown that staining the hearts with di-4-ANEPPS leads to only short-term sporadic prolongation of impulse conduction through atria and atrioventricular node. On the other hand, significant irreversible slowing of heart rate and ventricular conduction were found in stained hearts as compared to controls. In patch clamp experiments, significant inhibition of sodium current density was observed in differentiated NG108-15 cells stained by the dye. Although no significant differences in mean number of ventricular premature beats were found between the stained and the non-stained hearts in ischemia as well as in reperfusion, all abovementioned results indicate increased arrhythmogenicity. In isolated hearts during ischemia, prominent ischemic patterns appeared in the stained hearts with 3–4 min delay as compared to the non-stained ones. Moreover, the ischemic changes did not achieve the same magnitude as in controls even after 10 min of ischemia. It resulted in poor performance of ischemia detection by proposed EG parameters, as was quantified by receiver operating characteristics analysis.ConclusionOur results demonstrate significant direct irreversible effect of di-4-ANEPPS on spontaneous heart rate and ventricular impulse conduction in rabbit isolated heart model. Particularly, this should be considered when di-4-ANEPPS is used in ischemia studies in rabbit. Delayed attenuated response of such hearts to ischemia might lead to misinterpretation of obtained results. |
| first_indexed | 2024-12-21T15:49:23Z |
| format | Article |
| id | doaj.art-2c7409c77cca47959d57f7419e62f882 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-21T15:49:23Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-2c7409c77cca47959d57f7419e62f8822022-12-21T18:58:16ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-06-011210.3389/fphys.2021.667065667065Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated HeartMarina Ronzhina0Tibor Stracina1Lubica Lacinova2Katarina Ondacova3Michaela Pavlovicova4Lucie Marsanova5Radovan Smisek6Oto Janousek7Katerina Fialova8Jana Kolarova9Marie Novakova10Marie Novakova11Ivo Provaznik12Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, CzechiaDepartment of Physiology, Faculty of Medicine, Masaryk University, Brno, CzechiaCentre of Biosciences, Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, SlovakiaCentre of Biosciences, Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, SlovakiaCentre of Biosciences, Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, SlovakiaDepartment of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, CzechiaDepartment of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, CzechiaDepartment of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, CzechiaDepartment of Physiology, Faculty of Medicine, Masaryk University, Brno, CzechiaDepartment of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, CzechiaDepartment of Physiology, Faculty of Medicine, Masaryk University, Brno, CzechiaInternational Clinical Research Center, St. Anne’s University Hospital Brno, Brno, CzechiaDepartment of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, CzechiaAimsAlthough voltage-sensitive dye di-4-ANEPPS is a common tool for mapping cardiac electrical activity, reported effects on electrophysiological parameters are rather. The main goals of the study were to reveal effects of the dye on rabbit isolated heart and to verify, whether rabbit isolated heart stained with di-4-ANEPPS is a suitable tool for myocardial ischemia investigation.Methods and ResultsStudy involved experiments on stained (n = 9) and non-stained (n = 11) Langendorff perfused rabbit isolated hearts. Electrophysiological effects of the dye were evaluated by analysis of various electrogram (EG) parameters using common paired and unpaired statistical tests. It was shown that staining the hearts with di-4-ANEPPS leads to only short-term sporadic prolongation of impulse conduction through atria and atrioventricular node. On the other hand, significant irreversible slowing of heart rate and ventricular conduction were found in stained hearts as compared to controls. In patch clamp experiments, significant inhibition of sodium current density was observed in differentiated NG108-15 cells stained by the dye. Although no significant differences in mean number of ventricular premature beats were found between the stained and the non-stained hearts in ischemia as well as in reperfusion, all abovementioned results indicate increased arrhythmogenicity. In isolated hearts during ischemia, prominent ischemic patterns appeared in the stained hearts with 3–4 min delay as compared to the non-stained ones. Moreover, the ischemic changes did not achieve the same magnitude as in controls even after 10 min of ischemia. It resulted in poor performance of ischemia detection by proposed EG parameters, as was quantified by receiver operating characteristics analysis.ConclusionOur results demonstrate significant direct irreversible effect of di-4-ANEPPS on spontaneous heart rate and ventricular impulse conduction in rabbit isolated heart model. Particularly, this should be considered when di-4-ANEPPS is used in ischemia studies in rabbit. Delayed attenuated response of such hearts to ischemia might lead to misinterpretation of obtained results.https://www.frontiersin.org/articles/10.3389/fphys.2021.667065/fulldi-4-ANEPPSrabbit isolated heartmyocardial ischemiaelectrogram analysispatch-clampvoltage-sensitive dye |
| spellingShingle | Marina Ronzhina Tibor Stracina Lubica Lacinova Katarina Ondacova Michaela Pavlovicova Lucie Marsanova Radovan Smisek Oto Janousek Katerina Fialova Jana Kolarova Marie Novakova Marie Novakova Ivo Provaznik Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart Frontiers in Physiology di-4-ANEPPS rabbit isolated heart myocardial ischemia electrogram analysis patch-clamp voltage-sensitive dye |
| title | Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart |
| title_full | Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart |
| title_fullStr | Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart |
| title_full_unstemmed | Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart |
| title_short | Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart |
| title_sort | di 4 anepps modulates electrical activity and progress of myocardial ischemia in rabbit isolated heart |
| topic | di-4-ANEPPS rabbit isolated heart myocardial ischemia electrogram analysis patch-clamp voltage-sensitive dye |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2021.667065/full |
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