Dysregulated metal ion homeostasis underscores non-canonical function of CD8+ T cell during COVID-19
IntroductionSeveral efforts have been made to describe the complexity of T cell heterogeneity during the COVID-19 disease; however, there remain gaps in our understanding in terms of the granularity within.MethodsFor this attempt, we performed a single-cell transcriptomic analysis of 33 individuals...
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2023.1282390/full |
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| author | Kriti Khare Kriti Khare Partha Chattopadhyay Partha Chattopadhyay Priti Devi Priti Devi Priyanka Mehta Priyanka Mehta Aakarshan Raina Chinky Shiu Chen Liu Kishore Tardalkar Meghnad G. Joshi Rajesh Pandey Rajesh Pandey |
| author_facet | Kriti Khare Kriti Khare Partha Chattopadhyay Partha Chattopadhyay Priti Devi Priti Devi Priyanka Mehta Priyanka Mehta Aakarshan Raina Chinky Shiu Chen Liu Kishore Tardalkar Meghnad G. Joshi Rajesh Pandey Rajesh Pandey |
| author_sort | Kriti Khare |
| collection | DOAJ |
| description | IntroductionSeveral efforts have been made to describe the complexity of T cell heterogeneity during the COVID-19 disease; however, there remain gaps in our understanding in terms of the granularity within.MethodsFor this attempt, we performed a single-cell transcriptomic analysis of 33 individuals (4 healthy, 16 COVID-19 positive patients, and 13 COVID-19 recovered individuals).ResultsWe found CD8+ T cell-biased lymphopenia in COVID-19 patients compared to healthy and recovered individuals. We also found an optimal Th1/Th2 ratio, indicating an effective immune response during COVID-19. Expansion of activated CD4+ T and NK T was detected in the COVID-19-positive individuals. Surprisingly, we found cellular and metal ion homeostasis pathways enriched in the COVID-19-positive individuals compared to the healthy and recovered in the CD8+ T cell populations (CD8+ TCM and CD8+ TEM) as well as activated CD4+ T cells.DiscussionIn summary, the COVID-19-positive individuals exhibit a dynamic T cell mediated response. This response may have a possible association with the dysregulation of non-canonical pathways, including housekeeping functions in addition to the conventional antiviral immune response mediated by the T cell subpopulation. These findings considerably extend our insights into the heterogeneity of T cell response during and post-SARS-CoV-2 infection. |
| first_indexed | 2024-03-11T19:06:04Z |
| format | Article |
| id | doaj.art-2c7b86335a1f4c939f36f197a77aa56f |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-03-11T19:06:04Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-2c7b86335a1f4c939f36f197a77aa56f2023-10-10T07:21:21ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2023-10-011010.3389/fmed.2023.12823901282390Dysregulated metal ion homeostasis underscores non-canonical function of CD8+ T cell during COVID-19Kriti Khare0Kriti Khare1Partha Chattopadhyay2Partha Chattopadhyay3Priti Devi4Priti Devi5Priyanka Mehta6Priyanka Mehta7Aakarshan Raina8Chinky Shiu Chen Liu9Kishore Tardalkar10Meghnad G. Joshi11Rajesh Pandey12Rajesh Pandey13Division of Immunology and Infectious Disease Biology, INtegrative GENomics of HOst-PathogEn (INGEN-HOPE) Laboratory, CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), Delhi, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaDivision of Immunology and Infectious Disease Biology, INtegrative GENomics of HOst-PathogEn (INGEN-HOPE) Laboratory, CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), Delhi, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaDivision of Immunology and Infectious Disease Biology, INtegrative GENomics of HOst-PathogEn (INGEN-HOPE) Laboratory, CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), Delhi, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaDivision of Immunology and Infectious Disease Biology, INtegrative GENomics of HOst-PathogEn (INGEN-HOPE) Laboratory, CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), Delhi, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaDivision of Immunology and Infectious Disease Biology, INtegrative GENomics of HOst-PathogEn (INGEN-HOPE) Laboratory, CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), Delhi, IndiaDivision of Immunology and Infectious Disease Biology, INtegrative GENomics of HOst-PathogEn (INGEN-HOPE) Laboratory, CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), Delhi, IndiaD. Y. Patil Education Society, Institution Deemed to be University, Kolhapur, Maharashtra, IndiaDepartment of Stem Cells and Regenerative Medicine, D. Y. Patil Education Society, Deemed to be University, Kolhapur, Maharashtra, IndiaDivision of Immunology and Infectious Disease Biology, INtegrative GENomics of HOst-PathogEn (INGEN-HOPE) Laboratory, CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), Delhi, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaIntroductionSeveral efforts have been made to describe the complexity of T cell heterogeneity during the COVID-19 disease; however, there remain gaps in our understanding in terms of the granularity within.MethodsFor this attempt, we performed a single-cell transcriptomic analysis of 33 individuals (4 healthy, 16 COVID-19 positive patients, and 13 COVID-19 recovered individuals).ResultsWe found CD8+ T cell-biased lymphopenia in COVID-19 patients compared to healthy and recovered individuals. We also found an optimal Th1/Th2 ratio, indicating an effective immune response during COVID-19. Expansion of activated CD4+ T and NK T was detected in the COVID-19-positive individuals. Surprisingly, we found cellular and metal ion homeostasis pathways enriched in the COVID-19-positive individuals compared to the healthy and recovered in the CD8+ T cell populations (CD8+ TCM and CD8+ TEM) as well as activated CD4+ T cells.DiscussionIn summary, the COVID-19-positive individuals exhibit a dynamic T cell mediated response. This response may have a possible association with the dysregulation of non-canonical pathways, including housekeeping functions in addition to the conventional antiviral immune response mediated by the T cell subpopulation. These findings considerably extend our insights into the heterogeneity of T cell response during and post-SARS-CoV-2 infection.https://www.frontiersin.org/articles/10.3389/fmed.2023.1282390/fullsingle cell RNA-seqCOVID-19T cell heterogeneitynon-canonicalmetal ion |
| spellingShingle | Kriti Khare Kriti Khare Partha Chattopadhyay Partha Chattopadhyay Priti Devi Priti Devi Priyanka Mehta Priyanka Mehta Aakarshan Raina Chinky Shiu Chen Liu Kishore Tardalkar Meghnad G. Joshi Rajesh Pandey Rajesh Pandey Dysregulated metal ion homeostasis underscores non-canonical function of CD8+ T cell during COVID-19 Frontiers in Medicine single cell RNA-seq COVID-19 T cell heterogeneity non-canonical metal ion |
| title | Dysregulated metal ion homeostasis underscores non-canonical function of CD8+ T cell during COVID-19 |
| title_full | Dysregulated metal ion homeostasis underscores non-canonical function of CD8+ T cell during COVID-19 |
| title_fullStr | Dysregulated metal ion homeostasis underscores non-canonical function of CD8+ T cell during COVID-19 |
| title_full_unstemmed | Dysregulated metal ion homeostasis underscores non-canonical function of CD8+ T cell during COVID-19 |
| title_short | Dysregulated metal ion homeostasis underscores non-canonical function of CD8+ T cell during COVID-19 |
| title_sort | dysregulated metal ion homeostasis underscores non canonical function of cd8 t cell during covid 19 |
| topic | single cell RNA-seq COVID-19 T cell heterogeneity non-canonical metal ion |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2023.1282390/full |
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