Growth and alkaline digestive proteases activity of Parachromis dovii larvae fed live prey and formulated diets with different protein sources
ABSTRACT | Parachromis dovii is a carnivorous cichlid fish from Central America, which easily accepts formulated feeds since exogenous feeding starts; however, its larval growth is lower than that obtained using live food. For this reason, the effect of different dietary protein source combinations...
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| Format: | Article |
| Language: | English |
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Universidad Técnica de Manabí
2022-05-01
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| Series: | AquaTechnica |
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| Online Access: | https://revistas.utm.edu.ec/index.php/aquatechnica/article/view/4610 |
| _version_ | 1827379012291264512 |
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| author | Juan Bautista Ulloa Rojas Mauricio Herrera Campos Silvia Valverde Chavarria |
| author_facet | Juan Bautista Ulloa Rojas Mauricio Herrera Campos Silvia Valverde Chavarria |
| author_sort | Juan Bautista Ulloa Rojas |
| collection | DOAJ |
| description | ABSTRACT | Parachromis dovii is a carnivorous cichlid fish from Central America, which easily accepts formulated feeds since exogenous feeding starts; however, its larval growth is lower than that obtained using live food. For this reason, the effect of different dietary protein source combinations on growth and alkaline digestive proteases activity were tested in P. dovii larvae. The test protein mixtures were prepared using fish, meat and bone (tankage), and poultry by-product meals in different proportions (four experimental diets: D1-D4) and compared to a commercial product (Artemia cysts Vitellus) (VD) and Artemia nauplii as control (AD). At the end of the trial, larvae fed Artemia nauplii showed the best specific growth rate (SGR): 17.6% body weight per day (BW/d), contrary to the Vitellus diet which presented the lowest SGR (12.0% BW/d) (LSD, p<0.05). From the protein sources, D3 (with the highest content poultry by-products meal) showed the worst SGR (12.6% BW/d) while the other treatments gave similar values (D1: 13.5%, D2: 13.2% and D4: 13.7% BW/d). Survival was not affected by dietary treatment (over 93%, p>0.05). The lowest alkaline digestive protease activity was found six days after hatching (DAH), and it was similar among treatments. The protease activity increased up to 27 DAH, being higher with larvae fed Artemia nauplii than with the other treatments. There were no differences in protease activity among the protein mixture diets, nor were there differences when comparing it with the Vitellus, except at 20 DAH.
RESUMEN | Parachromis dovii es un pez cíclido carnívoro de Centroamérica, el cual acepta fácilmente alimento balanceado desde el momento en que se inicia su alimentación exógena; sin embargo, su crecimiento larvario es menor cuando se usa alimento balanceado que con el alimento vivo. Por esta razón, se evaluó el efecto de diferentes mezclas de proteínas en el alimento sobre el crecimiento y la actividad de las proteasas alcalinas digestivas en larvas de P. dovii. Las mezclas de proteínas en el alimento fueron preparadas con harina de pescado, carne y hueso, tortave (subproductos avícolas) (cuatro dietas, D1-D4), y comparadas con un producto comercial (Vitelo de quistes de Artemia, VD) y nauplios de Artemia como control (AD). Al final del ensayo, las larvas alimentadas con nauplios de Artemia presentaron el mejor crecimiento (tasa especifica de crecimiento (TEC): 17.6% peso corporal por día, PC/d) y el menor crecimiento se presentó con el Vitelo (12.0% PC/d) (LSD, p<0.05). De las fuentes de proteína, la D3 (con el mayor porcentaje de tortave) mostró la menor TEC, mientras que las otras mezclas proteicas dieron valores similares ((D1: 13,5%, D2: 13,2% and D4: 13,7% PC/d). La sobrevivencia (mayor a 93%) no fue afectada por los tratamientos evaluados (LSD, p>0.05). La actividad de las proteasas digestivas alcalinas mas bajo se encontró a los seis días después de la eclosión (DDE), y esta fue similar entre todos los tratamientos. La actividad de las proteasas se incrementó hasta los 27 DDE, siendo más alta con las larvas alimentadas con nauplios de Artemia que con los otros tratamientos. No se encontró diferencias en la actividad de las proteasas entre las dietas con mezclas de proteínas, ni tampoco hubo diferencias al compararla con la dieta Vitelo, excepto a los 20 DDE. |
| first_indexed | 2024-03-08T13:05:58Z |
| format | Article |
| id | doaj.art-2c7c3fa95dfa4e828eeff20e2a780909 |
| institution | Directory Open Access Journal |
| issn | 2737-6095 |
| language | English |
| last_indexed | 2024-03-08T13:05:58Z |
| publishDate | 2022-05-01 |
| publisher | Universidad Técnica de Manabí |
| record_format | Article |
| series | AquaTechnica |
| spelling | doaj.art-2c7c3fa95dfa4e828eeff20e2a7809092024-01-18T18:47:18ZengUniversidad Técnica de ManabíAquaTechnica2737-60952022-05-01427584https://doi.org/10.5281/zenodo.6845905Growth and alkaline digestive proteases activity of Parachromis dovii larvae fed live prey and formulated diets with different protein sources Juan Bautista Ulloa Rojas 0https://orcid.org/0000-0003-4464-1136Mauricio Herrera Campos 1https://orcid.org/0000-0003-3327-1950Silvia Valverde Chavarria 2https://orcid.org/0000-0003-2823-459XLaboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, 86 - 3000 Heredia, Costa RicaLaboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, 86 - 3000 Heredia, Costa RicaLaboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, 86 - 3000 Heredia, Costa RicaABSTRACT | Parachromis dovii is a carnivorous cichlid fish from Central America, which easily accepts formulated feeds since exogenous feeding starts; however, its larval growth is lower than that obtained using live food. For this reason, the effect of different dietary protein source combinations on growth and alkaline digestive proteases activity were tested in P. dovii larvae. The test protein mixtures were prepared using fish, meat and bone (tankage), and poultry by-product meals in different proportions (four experimental diets: D1-D4) and compared to a commercial product (Artemia cysts Vitellus) (VD) and Artemia nauplii as control (AD). At the end of the trial, larvae fed Artemia nauplii showed the best specific growth rate (SGR): 17.6% body weight per day (BW/d), contrary to the Vitellus diet which presented the lowest SGR (12.0% BW/d) (LSD, p<0.05). From the protein sources, D3 (with the highest content poultry by-products meal) showed the worst SGR (12.6% BW/d) while the other treatments gave similar values (D1: 13.5%, D2: 13.2% and D4: 13.7% BW/d). Survival was not affected by dietary treatment (over 93%, p>0.05). The lowest alkaline digestive protease activity was found six days after hatching (DAH), and it was similar among treatments. The protease activity increased up to 27 DAH, being higher with larvae fed Artemia nauplii than with the other treatments. There were no differences in protease activity among the protein mixture diets, nor were there differences when comparing it with the Vitellus, except at 20 DAH. RESUMEN | Parachromis dovii es un pez cíclido carnívoro de Centroamérica, el cual acepta fácilmente alimento balanceado desde el momento en que se inicia su alimentación exógena; sin embargo, su crecimiento larvario es menor cuando se usa alimento balanceado que con el alimento vivo. Por esta razón, se evaluó el efecto de diferentes mezclas de proteínas en el alimento sobre el crecimiento y la actividad de las proteasas alcalinas digestivas en larvas de P. dovii. Las mezclas de proteínas en el alimento fueron preparadas con harina de pescado, carne y hueso, tortave (subproductos avícolas) (cuatro dietas, D1-D4), y comparadas con un producto comercial (Vitelo de quistes de Artemia, VD) y nauplios de Artemia como control (AD). Al final del ensayo, las larvas alimentadas con nauplios de Artemia presentaron el mejor crecimiento (tasa especifica de crecimiento (TEC): 17.6% peso corporal por día, PC/d) y el menor crecimiento se presentó con el Vitelo (12.0% PC/d) (LSD, p<0.05). De las fuentes de proteína, la D3 (con el mayor porcentaje de tortave) mostró la menor TEC, mientras que las otras mezclas proteicas dieron valores similares ((D1: 13,5%, D2: 13,2% and D4: 13,7% PC/d). La sobrevivencia (mayor a 93%) no fue afectada por los tratamientos evaluados (LSD, p>0.05). La actividad de las proteasas digestivas alcalinas mas bajo se encontró a los seis días después de la eclosión (DDE), y esta fue similar entre todos los tratamientos. La actividad de las proteasas se incrementó hasta los 27 DDE, siendo más alta con las larvas alimentadas con nauplios de Artemia que con los otros tratamientos. No se encontró diferencias en la actividad de las proteasas entre las dietas con mezclas de proteínas, ni tampoco hubo diferencias al compararla con la dieta Vitelo, excepto a los 20 DDE.https://revistas.utm.edu.ec/index.php/aquatechnica/article/view/4610fish larvaeprotein sourcesenzymatic activityfeeding |
| spellingShingle | Juan Bautista Ulloa Rojas Mauricio Herrera Campos Silvia Valverde Chavarria Growth and alkaline digestive proteases activity of Parachromis dovii larvae fed live prey and formulated diets with different protein sources AquaTechnica fish larvae protein sources enzymatic activity feeding |
| title | Growth and alkaline digestive proteases activity of Parachromis dovii larvae fed live prey and formulated diets with different protein sources |
| title_full | Growth and alkaline digestive proteases activity of Parachromis dovii larvae fed live prey and formulated diets with different protein sources |
| title_fullStr | Growth and alkaline digestive proteases activity of Parachromis dovii larvae fed live prey and formulated diets with different protein sources |
| title_full_unstemmed | Growth and alkaline digestive proteases activity of Parachromis dovii larvae fed live prey and formulated diets with different protein sources |
| title_short | Growth and alkaline digestive proteases activity of Parachromis dovii larvae fed live prey and formulated diets with different protein sources |
| title_sort | growth and alkaline digestive proteases activity of parachromis dovii larvae fed live prey and formulated diets with different protein sources |
| topic | fish larvae protein sources enzymatic activity feeding |
| url | https://revistas.utm.edu.ec/index.php/aquatechnica/article/view/4610 |
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