A magic kick for regeneration: role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recovery
Abstract Background Injury of stem cell niches may disturb tissue homeostasis and regeneration coordinated by specific niche components. Yet, the mechanisms of stem cell niche restoration remain poorly understood. Herein, we examined the role of mesenchymal stromal cells (MSCs) as pivotal regulators...
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13287-019-1479-3 |
| _version_ | 1828783808309100544 |
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| author | Georgy Sagaradze Nataliya Basalova Vladimir Kirpatovsky Dmitry Ohobotov Peter Nimiritsky Olga Grigorieva Vladimir Popov Armais Kamalov Vsevolod Tkachuk Anastasia Efimenko |
| author_facet | Georgy Sagaradze Nataliya Basalova Vladimir Kirpatovsky Dmitry Ohobotov Peter Nimiritsky Olga Grigorieva Vladimir Popov Armais Kamalov Vsevolod Tkachuk Anastasia Efimenko |
| author_sort | Georgy Sagaradze |
| collection | DOAJ |
| description | Abstract Background Injury of stem cell niches may disturb tissue homeostasis and regeneration coordinated by specific niche components. Yet, the mechanisms of stem cell niche restoration remain poorly understood. Herein, we examined the role of mesenchymal stromal cells (MSCs) as pivotal regulators of stem cell niche recovery focusing on the effects of their secretome. Methods The spermatogonial stem cell (SSC) niche was selected as a model. SSC niches were injured by inducing abdominal cryptorchidism in rats. Briefly, testes of anesthetized rats were elevated into the abdominal cavity through the inguinal canal for 14 days. After descent of testes, MSC or MSC secretome treatment was applied to the animals by local subtunical injections. Results Local administration of MSC or MSC secretome was sufficient to recover spermatogenesis and production of functional germ cells. The effects of MSC and their secreted components were comparable, leading to restoration of Sertoli cell pools and recovery of Leydig cell secretory functions. Conclusion Our data suggest that MSCs mimic the functions of lost supportive cells within the stem cell niche, transiently providing paracrine stimuli for target cells and triggering tissue regenerative processes after damage. |
| first_indexed | 2024-12-11T23:19:01Z |
| format | Article |
| id | doaj.art-2c7d56ec7e8640b2af7ec36394f32401 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-11T23:19:01Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-2c7d56ec7e8640b2af7ec36394f324012022-12-22T00:46:24ZengBMCStem Cell Research & Therapy1757-65122019-11-0110111010.1186/s13287-019-1479-3A magic kick for regeneration: role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recoveryGeorgy Sagaradze0Nataliya Basalova1Vladimir Kirpatovsky2Dmitry Ohobotov3Peter Nimiritsky4Olga Grigorieva5Vladimir Popov6Armais Kamalov7Vsevolod Tkachuk8Anastasia Efimenko9Medical Research and Education Center, Lomonosov Moscow State UniversityMedical Research and Education Center, Lomonosov Moscow State UniversityMedical Research and Education Center, Lomonosov Moscow State UniversityMedical Research and Education Center, Lomonosov Moscow State UniversityMedical Research and Education Center, Lomonosov Moscow State UniversityMedical Research and Education Center, Lomonosov Moscow State UniversityFaculty of Medicine, Lomonosov Moscow State UniversityMedical Research and Education Center, Lomonosov Moscow State UniversityMedical Research and Education Center, Lomonosov Moscow State UniversityMedical Research and Education Center, Lomonosov Moscow State UniversityAbstract Background Injury of stem cell niches may disturb tissue homeostasis and regeneration coordinated by specific niche components. Yet, the mechanisms of stem cell niche restoration remain poorly understood. Herein, we examined the role of mesenchymal stromal cells (MSCs) as pivotal regulators of stem cell niche recovery focusing on the effects of their secretome. Methods The spermatogonial stem cell (SSC) niche was selected as a model. SSC niches were injured by inducing abdominal cryptorchidism in rats. Briefly, testes of anesthetized rats were elevated into the abdominal cavity through the inguinal canal for 14 days. After descent of testes, MSC or MSC secretome treatment was applied to the animals by local subtunical injections. Results Local administration of MSC or MSC secretome was sufficient to recover spermatogenesis and production of functional germ cells. The effects of MSC and their secreted components were comparable, leading to restoration of Sertoli cell pools and recovery of Leydig cell secretory functions. Conclusion Our data suggest that MSCs mimic the functions of lost supportive cells within the stem cell niche, transiently providing paracrine stimuli for target cells and triggering tissue regenerative processes after damage.http://link.springer.com/article/10.1186/s13287-019-1479-3Stem cell nicheSpermatogenesisMesenchymal stromal cellsMesenchymal stem cellsRegeneration |
| spellingShingle | Georgy Sagaradze Nataliya Basalova Vladimir Kirpatovsky Dmitry Ohobotov Peter Nimiritsky Olga Grigorieva Vladimir Popov Armais Kamalov Vsevolod Tkachuk Anastasia Efimenko A magic kick for regeneration: role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recovery Stem Cell Research & Therapy Stem cell niche Spermatogenesis Mesenchymal stromal cells Mesenchymal stem cells Regeneration |
| title | A magic kick for regeneration: role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recovery |
| title_full | A magic kick for regeneration: role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recovery |
| title_fullStr | A magic kick for regeneration: role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recovery |
| title_full_unstemmed | A magic kick for regeneration: role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recovery |
| title_short | A magic kick for regeneration: role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recovery |
| title_sort | magic kick for regeneration role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recovery |
| topic | Stem cell niche Spermatogenesis Mesenchymal stromal cells Mesenchymal stem cells Regeneration |
| url | http://link.springer.com/article/10.1186/s13287-019-1479-3 |
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