Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization
Critical size bone defects are regularly treated by auto- and allograft transplantation. However, such treatments require to harvest bone from patient donor sites, with often limited tissue availability or risk of donor site morbidity. Not requiring bone donation, three-dimensionally (3D) printed im...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00552/full |
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| author | Thomas Distler Niklas Fournier Alina Grünewald Christian Polley Hermann Seitz Rainer Detsch Aldo R. Boccaccini |
| author_facet | Thomas Distler Niklas Fournier Alina Grünewald Christian Polley Hermann Seitz Rainer Detsch Aldo R. Boccaccini |
| author_sort | Thomas Distler |
| collection | DOAJ |
| description | Critical size bone defects are regularly treated by auto- and allograft transplantation. However, such treatments require to harvest bone from patient donor sites, with often limited tissue availability or risk of donor site morbidity. Not requiring bone donation, three-dimensionally (3D) printed implants and biomaterial-based tissue engineering (TE) strategies promise to be the next generation therapies for bone regeneration. We present here polylactic acid (PLA)-bioactive glass (BG) composite scaffolds manufactured by fused deposition modeling (FDM), involving the fabrication of PLA-BG composite filaments which are used to 3D print controlled open-porous and osteoinductive scaffolds. We demonstrated the printability of PLA-BG filaments as well as the bioactivity and cytocompatibility of PLA-BG scaffolds using pre-osteoblast MC3T3E1 cells. Gene expression analyses indicated the beneficial impact of BG inclusions in FDM scaffolds regarding osteoinduction, as BG inclusions lead to increased osteogenic differentiation of human adipose-derived stem cells in comparison to pristine PLA. Our findings confirm that FDM is a convenient additive manufacturing technology to develop PLA-BG composite scaffolds suitable for bone tissue engineering. |
| first_indexed | 2024-12-14T22:08:39Z |
| format | Article |
| id | doaj.art-2c8039293fd04f62b34db76a417dcaac |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-14T22:08:39Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-2c8039293fd04f62b34db76a417dcaac2022-12-21T22:45:48ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-06-01810.3389/fbioe.2020.00552528469Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and CharacterizationThomas Distler0Niklas Fournier1Alina Grünewald2Christian Polley3Hermann Seitz4Rainer Detsch5Aldo R. Boccaccini6Department of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, GermanyChair of Microfluidics, Faculty of Mechanical Engineering and Marine Technology, University of Rostock, Rostock, GermanyChair of Microfluidics, Faculty of Mechanical Engineering and Marine Technology, University of Rostock, Rostock, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, GermanyCritical size bone defects are regularly treated by auto- and allograft transplantation. However, such treatments require to harvest bone from patient donor sites, with often limited tissue availability or risk of donor site morbidity. Not requiring bone donation, three-dimensionally (3D) printed implants and biomaterial-based tissue engineering (TE) strategies promise to be the next generation therapies for bone regeneration. We present here polylactic acid (PLA)-bioactive glass (BG) composite scaffolds manufactured by fused deposition modeling (FDM), involving the fabrication of PLA-BG composite filaments which are used to 3D print controlled open-porous and osteoinductive scaffolds. We demonstrated the printability of PLA-BG filaments as well as the bioactivity and cytocompatibility of PLA-BG scaffolds using pre-osteoblast MC3T3E1 cells. Gene expression analyses indicated the beneficial impact of BG inclusions in FDM scaffolds regarding osteoinduction, as BG inclusions lead to increased osteogenic differentiation of human adipose-derived stem cells in comparison to pristine PLA. Our findings confirm that FDM is a convenient additive manufacturing technology to develop PLA-BG composite scaffolds suitable for bone tissue engineering.https://www.frontiersin.org/article/10.3389/fbioe.2020.00552/full3D printingfused deposition modeling3D printing filamentsbioactive glasspolymer ceramic compositesbone tissue engineering |
| spellingShingle | Thomas Distler Niklas Fournier Alina Grünewald Christian Polley Hermann Seitz Rainer Detsch Aldo R. Boccaccini Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization Frontiers in Bioengineering and Biotechnology 3D printing fused deposition modeling 3D printing filaments bioactive glass polymer ceramic composites bone tissue engineering |
| title | Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization |
| title_full | Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization |
| title_fullStr | Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization |
| title_full_unstemmed | Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization |
| title_short | Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization |
| title_sort | polymer bioactive glass composite filaments for 3d scaffold manufacturing by fused deposition modeling fabrication and characterization |
| topic | 3D printing fused deposition modeling 3D printing filaments bioactive glass polymer ceramic composites bone tissue engineering |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00552/full |
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