Heterogeneity of Blood Vessels and Assessment of Microvessel Density-MVD in Gingivitis
Gingivitis is a very common oral disease highly prevalent in adults that, if left untreated, can progress to periodontitis. It involves a complex and slow interaction between the host response and the oral microbiome represented by the dental plaque. The inflammation of the gingiva is associated wit...
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| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/10/2758 |
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| author | Ciprian Roi Pușa Nela Gaje Raluca Amalia Ceaușu Alexandra Roi Laura Cristina Rusu Eugen Radu Boia Simina Boia Ruxandra Elena Luca Mircea Riviș |
| author_facet | Ciprian Roi Pușa Nela Gaje Raluca Amalia Ceaușu Alexandra Roi Laura Cristina Rusu Eugen Radu Boia Simina Boia Ruxandra Elena Luca Mircea Riviș |
| author_sort | Ciprian Roi |
| collection | DOAJ |
| description | Gingivitis is a very common oral disease highly prevalent in adults that, if left untreated, can progress to periodontitis. It involves a complex and slow interaction between the host response and the oral microbiome represented by the dental plaque. The inflammation of the gingiva is associated with the activation of pathological angiogenesis and the existence of a high number of newly formed blood vessels quantified as microvessel density (MVD). The present study includes a number of 51 gingival biopsies from patients with different gingival indexes (GI): GI = 0, <i>n</i> = 12; GI = 1, <i>n</i> = 15; GI = 2, <i>n</i> = 16; and GI = 3, <i>n</i> = 8, processed and stained with the routine hematoxylin–eosin method. The inflammatory infiltrate was scored, the blood vessels were detected with anti-CD34 antibody, and MVD was determined. Inflammatory changes were observed in 39 of the 51 cases included in our study. CD34 + vessels with normal morphological appearance were observed in all 12 cases of health gingiva. In cases of inflammatory lesions, the morphology of the blood vessels showed changes with the evolution of gingival lesions. In severe inflammation, a particular aspect was observed in the vessels, such as the presence of the phenomenon of intussusception. MVD increases with the severity of gingival lesions, with the highest density being observed in severe inflammation. |
| first_indexed | 2024-03-10T03:40:16Z |
| format | Article |
| id | doaj.art-2c85582ba2b3476c9cb1c95f836d2ed3 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T03:40:16Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-2c85582ba2b3476c9cb1c95f836d2ed32023-11-23T11:34:23ZengMDPI AGJournal of Clinical Medicine2077-03832022-05-011110275810.3390/jcm11102758Heterogeneity of Blood Vessels and Assessment of Microvessel Density-MVD in GingivitisCiprian Roi0Pușa Nela Gaje1Raluca Amalia Ceaușu2Alexandra Roi3Laura Cristina Rusu4Eugen Radu Boia5Simina Boia6Ruxandra Elena Luca7Mircea Riviș8Department of Anesthesiology and Oral Surgery, Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaDepartment of Microscopic Morphology and Histology, Angiogenesis Research Center, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaDepartment of Microscopic Morphology and Histology, Angiogenesis Research Center, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaDepartment of Oral Pathology, Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaDepartment of Oral Pathology, Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaDepartment of Ear Nose and Throat, “Victor Babeș” University of Medicine and Pharmacy Timisoara, 300041 Timisoara, RomaniaDepartment of Periodontology, “Victor Babeș” University of Medicine and Pharmacy Timisoara, 300041 Timisoara, RomaniaDepartment of Oral Rehabilitation and Dental Emergencies, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaDepartment of Anesthesiology and Oral Surgery, Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaGingivitis is a very common oral disease highly prevalent in adults that, if left untreated, can progress to periodontitis. It involves a complex and slow interaction between the host response and the oral microbiome represented by the dental plaque. The inflammation of the gingiva is associated with the activation of pathological angiogenesis and the existence of a high number of newly formed blood vessels quantified as microvessel density (MVD). The present study includes a number of 51 gingival biopsies from patients with different gingival indexes (GI): GI = 0, <i>n</i> = 12; GI = 1, <i>n</i> = 15; GI = 2, <i>n</i> = 16; and GI = 3, <i>n</i> = 8, processed and stained with the routine hematoxylin–eosin method. The inflammatory infiltrate was scored, the blood vessels were detected with anti-CD34 antibody, and MVD was determined. Inflammatory changes were observed in 39 of the 51 cases included in our study. CD34 + vessels with normal morphological appearance were observed in all 12 cases of health gingiva. In cases of inflammatory lesions, the morphology of the blood vessels showed changes with the evolution of gingival lesions. In severe inflammation, a particular aspect was observed in the vessels, such as the presence of the phenomenon of intussusception. MVD increases with the severity of gingival lesions, with the highest density being observed in severe inflammation.https://www.mdpi.com/2077-0383/11/10/2758gingivitisMVDangiogenesisCD34 |
| spellingShingle | Ciprian Roi Pușa Nela Gaje Raluca Amalia Ceaușu Alexandra Roi Laura Cristina Rusu Eugen Radu Boia Simina Boia Ruxandra Elena Luca Mircea Riviș Heterogeneity of Blood Vessels and Assessment of Microvessel Density-MVD in Gingivitis Journal of Clinical Medicine gingivitis MVD angiogenesis CD34 |
| title | Heterogeneity of Blood Vessels and Assessment of Microvessel Density-MVD in Gingivitis |
| title_full | Heterogeneity of Blood Vessels and Assessment of Microvessel Density-MVD in Gingivitis |
| title_fullStr | Heterogeneity of Blood Vessels and Assessment of Microvessel Density-MVD in Gingivitis |
| title_full_unstemmed | Heterogeneity of Blood Vessels and Assessment of Microvessel Density-MVD in Gingivitis |
| title_short | Heterogeneity of Blood Vessels and Assessment of Microvessel Density-MVD in Gingivitis |
| title_sort | heterogeneity of blood vessels and assessment of microvessel density mvd in gingivitis |
| topic | gingivitis MVD angiogenesis CD34 |
| url | https://www.mdpi.com/2077-0383/11/10/2758 |
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