Application of genome editing techniques to regulate gene expression in crops
Abstract Background Enhanced agricultural production is urgently required to meet the food demands of the increasing global population. Abundant genetic diversity is expected to accelerate crop development. In particular, the development of the CRISPR/Cas genome editing technology has greatly enhanc...
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-04786-2 |
| _version_ | 1797275269025759232 |
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| author | Huirong Dong |
| author_facet | Huirong Dong |
| author_sort | Huirong Dong |
| collection | DOAJ |
| description | Abstract Background Enhanced agricultural production is urgently required to meet the food demands of the increasing global population. Abundant genetic diversity is expected to accelerate crop development. In particular, the development of the CRISPR/Cas genome editing technology has greatly enhanced our ability to improve crop’s genetic diversity through direct artificial gene modification. However, recent studies have shown that most crop improvement efforts using CRISPR/Cas techniques have mainly focused on the coding regions, and there is a relatively lack of studies on the regulatory regions of gene expression. Results This review briefly summarizes the development of CRISPR/Cas system in the beginning. Subsequently, the importance of gene regulatory regions in plants is discussed. The review focuses on recent developments and applications of mutations in regulatory regions via CRISPR/Cas techniques in crop breeding. Conclusion Finally, an outline of perspectives for future crop breeding using genome editing technologies is provided. This review provides new research insights for crop improvement using genome editing techniques. |
| first_indexed | 2024-03-07T15:10:51Z |
| format | Article |
| id | doaj.art-2c8582f5c1144bb89043eccb74666b4a |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-07T15:10:51Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-2c8582f5c1144bb89043eccb74666b4a2024-03-05T18:37:51ZengBMCBMC Plant Biology1471-22292024-02-012411910.1186/s12870-024-04786-2Application of genome editing techniques to regulate gene expression in cropsHuirong Dong0College of Agronomy and Biotechnology, Yunnan Agriculture UniversityAbstract Background Enhanced agricultural production is urgently required to meet the food demands of the increasing global population. Abundant genetic diversity is expected to accelerate crop development. In particular, the development of the CRISPR/Cas genome editing technology has greatly enhanced our ability to improve crop’s genetic diversity through direct artificial gene modification. However, recent studies have shown that most crop improvement efforts using CRISPR/Cas techniques have mainly focused on the coding regions, and there is a relatively lack of studies on the regulatory regions of gene expression. Results This review briefly summarizes the development of CRISPR/Cas system in the beginning. Subsequently, the importance of gene regulatory regions in plants is discussed. The review focuses on recent developments and applications of mutations in regulatory regions via CRISPR/Cas techniques in crop breeding. Conclusion Finally, an outline of perspectives for future crop breeding using genome editing technologies is provided. This review provides new research insights for crop improvement using genome editing techniques.https://doi.org/10.1186/s12870-024-04786-2CRISPR/CasGenome editingCrop improvementRegulatory regions |
| spellingShingle | Huirong Dong Application of genome editing techniques to regulate gene expression in crops BMC Plant Biology CRISPR/Cas Genome editing Crop improvement Regulatory regions |
| title | Application of genome editing techniques to regulate gene expression in crops |
| title_full | Application of genome editing techniques to regulate gene expression in crops |
| title_fullStr | Application of genome editing techniques to regulate gene expression in crops |
| title_full_unstemmed | Application of genome editing techniques to regulate gene expression in crops |
| title_short | Application of genome editing techniques to regulate gene expression in crops |
| title_sort | application of genome editing techniques to regulate gene expression in crops |
| topic | CRISPR/Cas Genome editing Crop improvement Regulatory regions |
| url | https://doi.org/10.1186/s12870-024-04786-2 |
| work_keys_str_mv | AT huirongdong applicationofgenomeeditingtechniquestoregulategeneexpressionincrops |