Characterization of the YdeO regulon in Escherichia coli.
Enterobacteria are able to survive under stressful conditions within animals, such as acidic conditions in the stomach, bile salts during transfer to the intestine and anaerobic conditions within the intestine. The glutamate-dependent (GAD) system plays a major role in acid resistance in Escherichia...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4222967?pdf=render |
| _version_ | 1811269979473969152 |
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| author | Yuki Yamanaka Taku Oshima Akira Ishihama Kaneyoshi Yamamoto |
| author_facet | Yuki Yamanaka Taku Oshima Akira Ishihama Kaneyoshi Yamamoto |
| author_sort | Yuki Yamanaka |
| collection | DOAJ |
| description | Enterobacteria are able to survive under stressful conditions within animals, such as acidic conditions in the stomach, bile salts during transfer to the intestine and anaerobic conditions within the intestine. The glutamate-dependent (GAD) system plays a major role in acid resistance in Escherichia coli, and expression of the GAD system is controlled by the regulatory cascade consisting of EvgAS > YdeO > GadE. To understand the YdeO regulon in vivo, we used ChIP-chip to interrogate the E. coli genome for candidate YdeO binding sites. All of the seven operons identified by ChIP-chip as being potentially regulated by YdeO were confirmed as being under the direct control of YdeO using RT-qPCR, EMSA, DNaseI-footprinting and reporter assays. Within this YdeO regulon, we identified four stress-response transcription factors, DctR, NhaR, GadE, and GadW and enzymes for anaerobic respiration. Both GadE and GadW are involved in regulation of the GAD system and NhaR is an activator for the sodium/proton antiporter gene. In conjunction with co-transcribed Slp, DctR is involved in protection against metabolic endoproducts under acidic conditions. Taken all together, we suggest that YdeO is a key regulator of E. coli survival in both acidic and anaerobic conditions. |
| first_indexed | 2024-04-12T21:52:41Z |
| format | Article |
| id | doaj.art-2c8594c294ba49e69ed558d5cea017fc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T21:52:41Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2c8594c294ba49e69ed558d5cea017fc2022-12-22T03:15:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11196210.1371/journal.pone.0111962Characterization of the YdeO regulon in Escherichia coli.Yuki YamanakaTaku OshimaAkira IshihamaKaneyoshi YamamotoEnterobacteria are able to survive under stressful conditions within animals, such as acidic conditions in the stomach, bile salts during transfer to the intestine and anaerobic conditions within the intestine. The glutamate-dependent (GAD) system plays a major role in acid resistance in Escherichia coli, and expression of the GAD system is controlled by the regulatory cascade consisting of EvgAS > YdeO > GadE. To understand the YdeO regulon in vivo, we used ChIP-chip to interrogate the E. coli genome for candidate YdeO binding sites. All of the seven operons identified by ChIP-chip as being potentially regulated by YdeO were confirmed as being under the direct control of YdeO using RT-qPCR, EMSA, DNaseI-footprinting and reporter assays. Within this YdeO regulon, we identified four stress-response transcription factors, DctR, NhaR, GadE, and GadW and enzymes for anaerobic respiration. Both GadE and GadW are involved in regulation of the GAD system and NhaR is an activator for the sodium/proton antiporter gene. In conjunction with co-transcribed Slp, DctR is involved in protection against metabolic endoproducts under acidic conditions. Taken all together, we suggest that YdeO is a key regulator of E. coli survival in both acidic and anaerobic conditions.http://europepmc.org/articles/PMC4222967?pdf=render |
| spellingShingle | Yuki Yamanaka Taku Oshima Akira Ishihama Kaneyoshi Yamamoto Characterization of the YdeO regulon in Escherichia coli. PLoS ONE |
| title | Characterization of the YdeO regulon in Escherichia coli. |
| title_full | Characterization of the YdeO regulon in Escherichia coli. |
| title_fullStr | Characterization of the YdeO regulon in Escherichia coli. |
| title_full_unstemmed | Characterization of the YdeO regulon in Escherichia coli. |
| title_short | Characterization of the YdeO regulon in Escherichia coli. |
| title_sort | characterization of the ydeo regulon in escherichia coli |
| url | http://europepmc.org/articles/PMC4222967?pdf=render |
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