Discrimination of distinct chicken M cell subsets based on CSF1R expression
Abstract In mammals, a subset of follicle-associated epithelial (FAE) cells, known as M cells, conduct the transcytosis of antigens across the epithelium into the underlying lymphoid tissues. We previously revealed that M cells in the FAE of the chicken lung, bursa of Fabricius (bursa), and caecum b...
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| Format: | Article |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-59368-x |
| _version_ | 1797199450774437888 |
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| author | Safieh Zeinali Kate Sutton Masoud Ghaderi Zefreh Neil Mabbott Lonneke Vervelde |
| author_facet | Safieh Zeinali Kate Sutton Masoud Ghaderi Zefreh Neil Mabbott Lonneke Vervelde |
| author_sort | Safieh Zeinali |
| collection | DOAJ |
| description | Abstract In mammals, a subset of follicle-associated epithelial (FAE) cells, known as M cells, conduct the transcytosis of antigens across the epithelium into the underlying lymphoid tissues. We previously revealed that M cells in the FAE of the chicken lung, bursa of Fabricius (bursa), and caecum based on the expression of CSF1R. Here, we applied RNA-seq analysis on highly enriched CSF1R-expressing bursal M cells to investigate their transcriptome and identify novel chicken M cell-associated genes. Our data show that, like mammalian M cells, those in the FAE of the chicken bursa also express SOX8, MARCKSL1, TNFAIP2 and PRNP. Immunohistochemical analysis also confirmed the expression of SOX8 in CSF1R-expressing cells in the lung, bursa, and caecum. However, we found that many other mammalian M cell-associated genes such as SPIB and GP2 were not expressed by chicken M cells or represented in the chicken genome. Instead, we show bursal M cells express high levels of related genes such as SPI1. Whereas our data show that bursal M cells expressed CSF1R-highly, the M cells in the small intestine lacked CSF1R and both expressed SOX8. This study offers insights into the transcriptome of chicken M cells, revealing the expression of CSF1R in M cells is tissue-specific. |
| first_indexed | 2024-04-24T07:15:57Z |
| format | Article |
| id | doaj.art-2c8735dcb3774b07b33f5109e4472067 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T07:15:57Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2c8735dcb3774b07b33f5109e44720672024-04-21T11:16:48ZengNature PortfolioScientific Reports2045-23222024-04-0114111610.1038/s41598-024-59368-xDiscrimination of distinct chicken M cell subsets based on CSF1R expressionSafieh Zeinali0Kate Sutton1Masoud Ghaderi Zefreh2Neil Mabbott3Lonneke Vervelde4Division of Immunology, The Roslin Institute & Royal (Dick) School of Veterinary Studies, University of EdinburghDivision of Immunology, The Roslin Institute & Royal (Dick) School of Veterinary Studies, University of EdinburghDivision of Genetics and Genomics, The Roslin Institute & Royal (Dick) School of Veterinary Studies, University of EdinburghDivision of Immunology, The Roslin Institute & Royal (Dick) School of Veterinary Studies, University of EdinburghDivision of Immunology, The Roslin Institute & Royal (Dick) School of Veterinary Studies, University of EdinburghAbstract In mammals, a subset of follicle-associated epithelial (FAE) cells, known as M cells, conduct the transcytosis of antigens across the epithelium into the underlying lymphoid tissues. We previously revealed that M cells in the FAE of the chicken lung, bursa of Fabricius (bursa), and caecum based on the expression of CSF1R. Here, we applied RNA-seq analysis on highly enriched CSF1R-expressing bursal M cells to investigate their transcriptome and identify novel chicken M cell-associated genes. Our data show that, like mammalian M cells, those in the FAE of the chicken bursa also express SOX8, MARCKSL1, TNFAIP2 and PRNP. Immunohistochemical analysis also confirmed the expression of SOX8 in CSF1R-expressing cells in the lung, bursa, and caecum. However, we found that many other mammalian M cell-associated genes such as SPIB and GP2 were not expressed by chicken M cells or represented in the chicken genome. Instead, we show bursal M cells express high levels of related genes such as SPI1. Whereas our data show that bursal M cells expressed CSF1R-highly, the M cells in the small intestine lacked CSF1R and both expressed SOX8. This study offers insights into the transcriptome of chicken M cells, revealing the expression of CSF1R in M cells is tissue-specific.https://doi.org/10.1038/s41598-024-59368-xChickenFollicle-associated epitheliumInterfollicular epitheliumM cellsRNA-seq |
| spellingShingle | Safieh Zeinali Kate Sutton Masoud Ghaderi Zefreh Neil Mabbott Lonneke Vervelde Discrimination of distinct chicken M cell subsets based on CSF1R expression Scientific Reports Chicken Follicle-associated epithelium Interfollicular epithelium M cells RNA-seq |
| title | Discrimination of distinct chicken M cell subsets based on CSF1R expression |
| title_full | Discrimination of distinct chicken M cell subsets based on CSF1R expression |
| title_fullStr | Discrimination of distinct chicken M cell subsets based on CSF1R expression |
| title_full_unstemmed | Discrimination of distinct chicken M cell subsets based on CSF1R expression |
| title_short | Discrimination of distinct chicken M cell subsets based on CSF1R expression |
| title_sort | discrimination of distinct chicken m cell subsets based on csf1r expression |
| topic | Chicken Follicle-associated epithelium Interfollicular epithelium M cells RNA-seq |
| url | https://doi.org/10.1038/s41598-024-59368-x |
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