LED arrays as cost effective and efficient light sources for widefield microscopy.
New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, diffi...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-05-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18478056/?tool=EBI |
| _version_ | 1818826707980779520 |
|---|---|
| author | Dinu F Albeanu Edward Soucy Tomokazu F Sato Markus Meister Venkatesh N Murthy |
| author_facet | Dinu F Albeanu Edward Soucy Tomokazu F Sato Markus Meister Venkatesh N Murthy |
| author_sort | Dinu F Albeanu |
| collection | DOAJ |
| description | New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes (LEDs). We also provide examples of its applicability to biological fluorescence imaging. |
| first_indexed | 2024-12-19T00:31:57Z |
| format | Article |
| id | doaj.art-2c8738b4ebea4734a1e6ba4d5c63aba6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T00:31:57Z |
| publishDate | 2008-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2c8738b4ebea4734a1e6ba4d5c63aba62022-12-21T20:45:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-05-0135e214610.1371/journal.pone.0002146LED arrays as cost effective and efficient light sources for widefield microscopy.Dinu F AlbeanuEdward SoucyTomokazu F SatoMarkus MeisterVenkatesh N MurthyNew developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes (LEDs). We also provide examples of its applicability to biological fluorescence imaging.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18478056/?tool=EBI |
| spellingShingle | Dinu F Albeanu Edward Soucy Tomokazu F Sato Markus Meister Venkatesh N Murthy LED arrays as cost effective and efficient light sources for widefield microscopy. PLoS ONE |
| title | LED arrays as cost effective and efficient light sources for widefield microscopy. |
| title_full | LED arrays as cost effective and efficient light sources for widefield microscopy. |
| title_fullStr | LED arrays as cost effective and efficient light sources for widefield microscopy. |
| title_full_unstemmed | LED arrays as cost effective and efficient light sources for widefield microscopy. |
| title_short | LED arrays as cost effective and efficient light sources for widefield microscopy. |
| title_sort | led arrays as cost effective and efficient light sources for widefield microscopy |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18478056/?tool=EBI |
| work_keys_str_mv | AT dinufalbeanu ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy AT edwardsoucy ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy AT tomokazufsato ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy AT markusmeister ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy AT venkateshnmurthy ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy |