Fumigant Antifungal Activity via Reactive Oxygen Species of <i>Thymus vulgaris</i> and <i>Satureja hortensis</i> Essential Oils and Constituents against <i>Raffaelea quercus-mongolicae</i> and <i>Rhizoctonia solani</i>
In this study, the fumigant antifungal activity of 10 Lamiaceae plant essential oils was evaluated against two phytopathogenic fungi, <i>Raffaelea quercus-mongolicae,</i> and <i>Rhizoctonia solani.</i> Among the tested essential oils, thyme white (<i>Thymus vulgaris<...
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| Format: | Article |
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MDPI AG
2019-10-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/10/561 |
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| author | Jeong-Eun Kim Ji-Eun Lee Min-Jung Huh Sung-Chan Lee Seon-Mi Seo Jun Hyeong Kwon Il-Kwon Park |
| author_facet | Jeong-Eun Kim Ji-Eun Lee Min-Jung Huh Sung-Chan Lee Seon-Mi Seo Jun Hyeong Kwon Il-Kwon Park |
| author_sort | Jeong-Eun Kim |
| collection | DOAJ |
| description | In this study, the fumigant antifungal activity of 10 Lamiaceae plant essential oils was evaluated against two phytopathogenic fungi, <i>Raffaelea quercus-mongolicae,</i> and <i>Rhizoctonia solani.</i> Among the tested essential oils, thyme white (<i>Thymus vulgaris</i>) and summer savory (<i>Satureja hortensis</i>) essential oils exhibited the strongest fumigant antifungal activity against the phytopathogenic fungi. We analyzed the chemical composition of two active essential oils and tested the fumigant antifungal activities of the identified compounds. Among the tested compounds, thymol and carvacrol had potent fumigant antifungal activity. We observed reactive oxygen species (ROS) generation in two fungi treated with thymol and carvacrol. Confocal laser scanning microscopy images of fungi stained with propidium iodide showed that thymol and carvacrol disrupted fungal cell membranes. Our results indicated that ROS generated by thymol and carvacrol damaged the cell membrane of <i>R. querqus-mongolicae</i> and <i>R. solani</i>, causing cell death. |
| first_indexed | 2024-12-11T10:49:47Z |
| format | Article |
| id | doaj.art-2c87f63768594e4bb0a1be8f29012caa |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-11T10:49:47Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-2c87f63768594e4bb0a1be8f29012caa2022-12-22T01:10:21ZengMDPI AGBiomolecules2218-273X2019-10-0191056110.3390/biom9100561biom9100561Fumigant Antifungal Activity via Reactive Oxygen Species of <i>Thymus vulgaris</i> and <i>Satureja hortensis</i> Essential Oils and Constituents against <i>Raffaelea quercus-mongolicae</i> and <i>Rhizoctonia solani</i>Jeong-Eun Kim0Ji-Eun Lee1Min-Jung Huh2Sung-Chan Lee3Seon-Mi Seo4Jun Hyeong Kwon5Il-Kwon Park6Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaIn this study, the fumigant antifungal activity of 10 Lamiaceae plant essential oils was evaluated against two phytopathogenic fungi, <i>Raffaelea quercus-mongolicae,</i> and <i>Rhizoctonia solani.</i> Among the tested essential oils, thyme white (<i>Thymus vulgaris</i>) and summer savory (<i>Satureja hortensis</i>) essential oils exhibited the strongest fumigant antifungal activity against the phytopathogenic fungi. We analyzed the chemical composition of two active essential oils and tested the fumigant antifungal activities of the identified compounds. Among the tested compounds, thymol and carvacrol had potent fumigant antifungal activity. We observed reactive oxygen species (ROS) generation in two fungi treated with thymol and carvacrol. Confocal laser scanning microscopy images of fungi stained with propidium iodide showed that thymol and carvacrol disrupted fungal cell membranes. Our results indicated that ROS generated by thymol and carvacrol damaged the cell membrane of <i>R. querqus-mongolicae</i> and <i>R. solani</i>, causing cell death.https://www.mdpi.com/2218-273X/9/10/561<i>thymus vulgaris</i><i>satureja hortensis</i>antifungal activityreactive oxygen speciesthymolcarvacrol |
| spellingShingle | Jeong-Eun Kim Ji-Eun Lee Min-Jung Huh Sung-Chan Lee Seon-Mi Seo Jun Hyeong Kwon Il-Kwon Park Fumigant Antifungal Activity via Reactive Oxygen Species of <i>Thymus vulgaris</i> and <i>Satureja hortensis</i> Essential Oils and Constituents against <i>Raffaelea quercus-mongolicae</i> and <i>Rhizoctonia solani</i> Biomolecules <i>thymus vulgaris</i> <i>satureja hortensis</i> antifungal activity reactive oxygen species thymol carvacrol |
| title | Fumigant Antifungal Activity via Reactive Oxygen Species of <i>Thymus vulgaris</i> and <i>Satureja hortensis</i> Essential Oils and Constituents against <i>Raffaelea quercus-mongolicae</i> and <i>Rhizoctonia solani</i> |
| title_full | Fumigant Antifungal Activity via Reactive Oxygen Species of <i>Thymus vulgaris</i> and <i>Satureja hortensis</i> Essential Oils and Constituents against <i>Raffaelea quercus-mongolicae</i> and <i>Rhizoctonia solani</i> |
| title_fullStr | Fumigant Antifungal Activity via Reactive Oxygen Species of <i>Thymus vulgaris</i> and <i>Satureja hortensis</i> Essential Oils and Constituents against <i>Raffaelea quercus-mongolicae</i> and <i>Rhizoctonia solani</i> |
| title_full_unstemmed | Fumigant Antifungal Activity via Reactive Oxygen Species of <i>Thymus vulgaris</i> and <i>Satureja hortensis</i> Essential Oils and Constituents against <i>Raffaelea quercus-mongolicae</i> and <i>Rhizoctonia solani</i> |
| title_short | Fumigant Antifungal Activity via Reactive Oxygen Species of <i>Thymus vulgaris</i> and <i>Satureja hortensis</i> Essential Oils and Constituents against <i>Raffaelea quercus-mongolicae</i> and <i>Rhizoctonia solani</i> |
| title_sort | fumigant antifungal activity via reactive oxygen species of i thymus vulgaris i and i satureja hortensis i essential oils and constituents against i raffaelea quercus mongolicae i and i rhizoctonia solani i |
| topic | <i>thymus vulgaris</i> <i>satureja hortensis</i> antifungal activity reactive oxygen species thymol carvacrol |
| url | https://www.mdpi.com/2218-273X/9/10/561 |
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