Investigation of Antibiotic Resistance Genes in Carbapenem Resistant Acinetobacter baumannii Strains Isolated from Patients Followed Up in the Intensive Care Unit
Introduction: Acinetobacter species are opportunistic pathogens that cause epidemics in intensive care units. The widespread and improper use of broad-spectrum antibiotics in hospitals has led to the development of resistance to many antibiotics in these bacteria. The most important resistance mecha...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bilimsel Tip Yayinevi
2021-06-01
|
| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://floradergisi.org/managete/fu_folder/2021-02/311-322%20Gulsen%20iskender.pdf |
| _version_ | 1797920562331254784 |
|---|---|
| author | Öznur Güneş Gülşen İskender Sabahat Çeken Özlem Ünaldı Ece Dirim Rıza Durmaz Mustafa Ertek |
| author_facet | Öznur Güneş Gülşen İskender Sabahat Çeken Özlem Ünaldı Ece Dirim Rıza Durmaz Mustafa Ertek |
| author_sort | Öznur Güneş |
| collection | DOAJ |
| description | Introduction: Acinetobacter species are opportunistic pathogens that cause epidemics in intensive care units. The widespread and improper use of broad-spectrum antibiotics in hospitals has led to the development of resistance to many antibiotics in these bacteria. The most important resistance mechanisms are beta-lactamase production, changes in cell wall channels, and efflux pump activity. The synthesis of OXA type beta-lactamases and metallo-betalactamases plays an important role in carbapenem resistance. The aim of our study was to identify the genes responsible for resistance in carbapenem-resistant Acinetobacter baumannii strains and to determine its relationship with previously used antibiotics to guide rational antibiotic use.
Materials and Methods: Forty-seven carbapenem-resistant A. baumannii strains grown in samples taken under appropriate conditions from patients followed in the intensive care unit were evaluated. Genes encoding OXA-50-51-55-24-23-58, VIM, IMP, KPC and NDM enzymes were investigated using the multiplex polymerase chain reaction (PCR).
Results: Mean age of the patients was 67.3 years (24-97 years), and 25 (53.2%) were females. Of the 47 isolates, 22 were extensively drug-resistant (XDR), 23 were multidrug-resistant (MDR) and 2 were pandrug-resistant (PDR) strains. The time from admission to intensive care unit until A. baumannii isolation in culture was 15.6 ± 9.7 days. blaOXA-23 and blaOXA-51 genes were detected in all strains, other genes were not detected. Before the isolation of carbapenem-resistant A. baumannii, carbapenem group antibiotics has been used in a statistically significant proportion of the patients [31 (66%) vs 16 (34%)] (p= 0.04).
Conclusion: Since blaOXA-51 is a gene found intrinsically in A. baumannii strains, blaOXA-23 is the gene responsible for carbapenem resistance in these strains in our hospital. We believe that it would be beneficial to carry out molecular epidemiological studies in order to monitor antibiotic susceptibility patterns and reveal new resistance mechanisms in treatment centers in order to take necessary precautions against pathogens such as A. baumannii in a timely manner. |
| first_indexed | 2024-04-10T14:04:06Z |
| format | Article |
| id | doaj.art-2c88ac6af6444e2eb02f830ec70f4e2f |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T14:04:06Z |
| publishDate | 2021-06-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-2c88ac6af6444e2eb02f830ec70f4e2f2023-02-15T16:10:06ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2021-06-0126231132210.5578/flora.20219810Investigation of Antibiotic Resistance Genes in Carbapenem Resistant Acinetobacter baumannii Strains Isolated from Patients Followed Up in the Intensive Care UnitÖznur Güneş0https://orcid.org/0000-0002-1213-3068Gülşen İskender1https://orcid.org/0000-0001-7619-1366Sabahat Çeken2https://orcid.org/0000-0002-3679-0978Özlem Ünaldı3https://orcid.org/0000-0002-5560-6558Ece Dirim4https://orcid.org/0000-0002-7087-2539Rıza Durmaz5https://orcid.org/0000-0001-6561-778XMustafa Ertek6https://orcid.org/0000-0002-9397-8432Clinic of Infectious Diseases and Clinical Microbiology, Midyat State Hospital, Mardin, TurkeyClinic of Infectious Diseases and Clinical Microbiology, Dr. Abdurrahman Yurtaslan Ankara Oncology Training and Research Hospital, Ankara, TurkeyClinic of Infectious Diseases and Clinical Microbiology, Dr. Abdurrahman Yurtaslan Ankara Oncology Training and Research Hospital, Ankara, TurkeyGeneral Directorate of Public Health, National Molecular Microbiology Reference Laboratory, Ankara, TurkeyLaboratory of Medical Microbiology, Dr. Abdurrahman Yurtaslan Ankara Oncology Training and Research Hospital, Ankara, TurkeyDepartment of Medical Microbiology, Yıldırım Beyazıt University Faculty of Medicine, Ankara, TurkeyClinic of Infectious Diseases and Clinical Microbiology, Dr. Abdurrahman Yurtaslan Ankara Oncology Training and Research Hospital, Ankara, TurkeyIntroduction: Acinetobacter species are opportunistic pathogens that cause epidemics in intensive care units. The widespread and improper use of broad-spectrum antibiotics in hospitals has led to the development of resistance to many antibiotics in these bacteria. The most important resistance mechanisms are beta-lactamase production, changes in cell wall channels, and efflux pump activity. The synthesis of OXA type beta-lactamases and metallo-betalactamases plays an important role in carbapenem resistance. The aim of our study was to identify the genes responsible for resistance in carbapenem-resistant Acinetobacter baumannii strains and to determine its relationship with previously used antibiotics to guide rational antibiotic use. Materials and Methods: Forty-seven carbapenem-resistant A. baumannii strains grown in samples taken under appropriate conditions from patients followed in the intensive care unit were evaluated. Genes encoding OXA-50-51-55-24-23-58, VIM, IMP, KPC and NDM enzymes were investigated using the multiplex polymerase chain reaction (PCR). Results: Mean age of the patients was 67.3 years (24-97 years), and 25 (53.2%) were females. Of the 47 isolates, 22 were extensively drug-resistant (XDR), 23 were multidrug-resistant (MDR) and 2 were pandrug-resistant (PDR) strains. The time from admission to intensive care unit until A. baumannii isolation in culture was 15.6 ± 9.7 days. blaOXA-23 and blaOXA-51 genes were detected in all strains, other genes were not detected. Before the isolation of carbapenem-resistant A. baumannii, carbapenem group antibiotics has been used in a statistically significant proportion of the patients [31 (66%) vs 16 (34%)] (p= 0.04). Conclusion: Since blaOXA-51 is a gene found intrinsically in A. baumannii strains, blaOXA-23 is the gene responsible for carbapenem resistance in these strains in our hospital. We believe that it would be beneficial to carry out molecular epidemiological studies in order to monitor antibiotic susceptibility patterns and reveal new resistance mechanisms in treatment centers in order to take necessary precautions against pathogens such as A. baumannii in a timely manner.http://floradergisi.org/managete/fu_folder/2021-02/311-322%20Gulsen%20iskender.pdfacinetobacter baumanniicarbapenem resistanceresistance genesintensive care unit |
| spellingShingle | Öznur Güneş Gülşen İskender Sabahat Çeken Özlem Ünaldı Ece Dirim Rıza Durmaz Mustafa Ertek Investigation of Antibiotic Resistance Genes in Carbapenem Resistant Acinetobacter baumannii Strains Isolated from Patients Followed Up in the Intensive Care Unit Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi acinetobacter baumannii carbapenem resistance resistance genes intensive care unit |
| title | Investigation of Antibiotic Resistance Genes in Carbapenem Resistant Acinetobacter baumannii Strains Isolated from Patients Followed Up in the Intensive Care Unit |
| title_full | Investigation of Antibiotic Resistance Genes in Carbapenem Resistant Acinetobacter baumannii Strains Isolated from Patients Followed Up in the Intensive Care Unit |
| title_fullStr | Investigation of Antibiotic Resistance Genes in Carbapenem Resistant Acinetobacter baumannii Strains Isolated from Patients Followed Up in the Intensive Care Unit |
| title_full_unstemmed | Investigation of Antibiotic Resistance Genes in Carbapenem Resistant Acinetobacter baumannii Strains Isolated from Patients Followed Up in the Intensive Care Unit |
| title_short | Investigation of Antibiotic Resistance Genes in Carbapenem Resistant Acinetobacter baumannii Strains Isolated from Patients Followed Up in the Intensive Care Unit |
| title_sort | investigation of antibiotic resistance genes in carbapenem resistant acinetobacter baumannii strains isolated from patients followed up in the intensive care unit |
| topic | acinetobacter baumannii carbapenem resistance resistance genes intensive care unit |
| url | http://floradergisi.org/managete/fu_folder/2021-02/311-322%20Gulsen%20iskender.pdf |
| work_keys_str_mv | AT oznurgunes investigationofantibioticresistancegenesincarbapenemresistantacinetobacterbaumanniistrainsisolatedfrompatientsfollowedupintheintensivecareunit AT gulseniskender investigationofantibioticresistancegenesincarbapenemresistantacinetobacterbaumanniistrainsisolatedfrompatientsfollowedupintheintensivecareunit AT sabahatceken investigationofantibioticresistancegenesincarbapenemresistantacinetobacterbaumanniistrainsisolatedfrompatientsfollowedupintheintensivecareunit AT ozlemunaldı investigationofantibioticresistancegenesincarbapenemresistantacinetobacterbaumanniistrainsisolatedfrompatientsfollowedupintheintensivecareunit AT ecedirim investigationofantibioticresistancegenesincarbapenemresistantacinetobacterbaumanniistrainsisolatedfrompatientsfollowedupintheintensivecareunit AT rızadurmaz investigationofantibioticresistancegenesincarbapenemresistantacinetobacterbaumanniistrainsisolatedfrompatientsfollowedupintheintensivecareunit AT mustafaertek investigationofantibioticresistancegenesincarbapenemresistantacinetobacterbaumanniistrainsisolatedfrompatientsfollowedupintheintensivecareunit |