ANTIBACTERIAL ACTIVITY AND TLC-BIOAUTOGRAPHY ANALYSIS OF THE ACTIVE FRACTIONS OF Muntingia calabura L. LEAVES AGAINST Staphylococcus aureus
This study aims to determine the antibacterial activity and TLC-bioautography profile of the active fractions of Muntingia calabura L. leaves against Staphylococcus aureus. Muntingia calabura L. leaves were macerated with ethanol 96% then fractionated with n-hexane, ethyl acetate, and methanol solve...
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| Format: | Article |
| Language: | English |
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Universitas Sanata Dharma
2020-11-01
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| Series: | Jurnal Farmasi Sains dan Komunitas |
| Subjects: | |
| Online Access: | https://e-journal.usd.ac.id/index.php/JFSK/article/view/2362 |
| _version_ | 1797785711849504768 |
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| author | Rizky H. Mawardi Nanik Sulistyani Nurkhasanah Nurkhasanah Ricke Desyratnaputri |
| author_facet | Rizky H. Mawardi Nanik Sulistyani Nurkhasanah Nurkhasanah Ricke Desyratnaputri |
| author_sort | Rizky H. Mawardi |
| collection | DOAJ |
| description | This study aims to determine the antibacterial activity and TLC-bioautography profile of the active fractions of Muntingia calabura L. leaves against Staphylococcus aureus. Muntingia calabura L. leaves were macerated with ethanol 96% then fractionated with n-hexane, ethyl acetate, and methanol solvent, respectively. The antibacterial activity was tested by the Kirby-Bauer method to determine the most active fraction and the lowest concentration that inhibited the growth of Staphylococcus aureus. TLC-bioautography was tested using chloroform: ethyl acetate (2:8) as the mobile phase and silica gel F254 as the stationary phase. Antibacterial activity test of n-hexane and ethyl acetate fractions at a concentration of 10% w/v showed activities with inhibition zone diameter of 0.330.288 and 9.665.77 mm, respectively. At the same time, the methanol fraction showed no activity. The lowest concentration of ethyl acetate fraction which still showed the inhibition zone was 0.312% w/v. The TLC-bioautography profile showed active spots with an Rf value of 0.82 and had an inhibitory zone diameter of 4.0130.864 mm. It can be concluded that the ethyl acetate fraction was the most active fraction that inhibited the growth of Staphylococcus aureus and had one active spot on the bioautography test. |
| first_indexed | 2024-03-13T00:58:49Z |
| format | Article |
| id | doaj.art-2c88f4f6f5ac4b57ac0ba1b79e8aa7bf |
| institution | Directory Open Access Journal |
| issn | 1693-5683 2527-7146 |
| language | English |
| last_indexed | 2024-03-13T00:58:49Z |
| publishDate | 2020-11-01 |
| publisher | Universitas Sanata Dharma |
| record_format | Article |
| series | Jurnal Farmasi Sains dan Komunitas |
| spelling | doaj.art-2c88f4f6f5ac4b57ac0ba1b79e8aa7bf2023-07-06T14:02:20ZengUniversitas Sanata DharmaJurnal Farmasi Sains dan Komunitas1693-56832527-71462020-11-01172697510.24071/jpsc.0023621809ANTIBACTERIAL ACTIVITY AND TLC-BIOAUTOGRAPHY ANALYSIS OF THE ACTIVE FRACTIONS OF Muntingia calabura L. LEAVES AGAINST Staphylococcus aureusRizky H. Mawardi0Nanik Sulistyani1Nurkhasanah Nurkhasanah2Ricke Desyratnaputri3Ahmad Dahlan UniversityAhmad Dahlan UniversityAhmad Dahlan UniversityAhmad Dahlan UniversityThis study aims to determine the antibacterial activity and TLC-bioautography profile of the active fractions of Muntingia calabura L. leaves against Staphylococcus aureus. Muntingia calabura L. leaves were macerated with ethanol 96% then fractionated with n-hexane, ethyl acetate, and methanol solvent, respectively. The antibacterial activity was tested by the Kirby-Bauer method to determine the most active fraction and the lowest concentration that inhibited the growth of Staphylococcus aureus. TLC-bioautography was tested using chloroform: ethyl acetate (2:8) as the mobile phase and silica gel F254 as the stationary phase. Antibacterial activity test of n-hexane and ethyl acetate fractions at a concentration of 10% w/v showed activities with inhibition zone diameter of 0.330.288 and 9.665.77 mm, respectively. At the same time, the methanol fraction showed no activity. The lowest concentration of ethyl acetate fraction which still showed the inhibition zone was 0.312% w/v. The TLC-bioautography profile showed active spots with an Rf value of 0.82 and had an inhibitory zone diameter of 4.0130.864 mm. It can be concluded that the ethyl acetate fraction was the most active fraction that inhibited the growth of Staphylococcus aureus and had one active spot on the bioautography test.https://e-journal.usd.ac.id/index.php/JFSK/article/view/2362antibacterialmuntingia calabura l. leafstaphylococcus aureustlc- bioautography. |
| spellingShingle | Rizky H. Mawardi Nanik Sulistyani Nurkhasanah Nurkhasanah Ricke Desyratnaputri ANTIBACTERIAL ACTIVITY AND TLC-BIOAUTOGRAPHY ANALYSIS OF THE ACTIVE FRACTIONS OF Muntingia calabura L. LEAVES AGAINST Staphylococcus aureus Jurnal Farmasi Sains dan Komunitas antibacterial muntingia calabura l. leaf staphylococcus aureus tlc- bioautography. |
| title | ANTIBACTERIAL ACTIVITY AND TLC-BIOAUTOGRAPHY ANALYSIS OF THE ACTIVE FRACTIONS OF Muntingia calabura L. LEAVES AGAINST Staphylococcus aureus |
| title_full | ANTIBACTERIAL ACTIVITY AND TLC-BIOAUTOGRAPHY ANALYSIS OF THE ACTIVE FRACTIONS OF Muntingia calabura L. LEAVES AGAINST Staphylococcus aureus |
| title_fullStr | ANTIBACTERIAL ACTIVITY AND TLC-BIOAUTOGRAPHY ANALYSIS OF THE ACTIVE FRACTIONS OF Muntingia calabura L. LEAVES AGAINST Staphylococcus aureus |
| title_full_unstemmed | ANTIBACTERIAL ACTIVITY AND TLC-BIOAUTOGRAPHY ANALYSIS OF THE ACTIVE FRACTIONS OF Muntingia calabura L. LEAVES AGAINST Staphylococcus aureus |
| title_short | ANTIBACTERIAL ACTIVITY AND TLC-BIOAUTOGRAPHY ANALYSIS OF THE ACTIVE FRACTIONS OF Muntingia calabura L. LEAVES AGAINST Staphylococcus aureus |
| title_sort | antibacterial activity and tlc bioautography analysis of the active fractions of muntingia calabura l leaves against staphylococcus aureus |
| topic | antibacterial muntingia calabura l. leaf staphylococcus aureus tlc- bioautography. |
| url | https://e-journal.usd.ac.id/index.php/JFSK/article/view/2362 |
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