Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base
In the present study, multiplication efficiency of Alstroemeria ligtu hybrid was investigated. Bases of the first seedling leaves grown in vitro were used as initial explants. The explants were cultured in the MS media containing 3% sucrose, 0.7% agar at pH 5.8, five N6-benzylaminopurine (BAP) conce...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2013-12-01
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| Series: | Journal of Horticultural Research |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/johr-2013-0018 |
| _version_ | 1818340353777860608 |
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| author | Nasri Fardin Mortazavi Seyed Najmedin Ghaderi Nasser Javadi Taimoor |
| author_facet | Nasri Fardin Mortazavi Seyed Najmedin Ghaderi Nasser Javadi Taimoor |
| author_sort | Nasri Fardin |
| collection | DOAJ |
| description | In the present study, multiplication efficiency of Alstroemeria ligtu hybrid was investigated. Bases of the first seedling leaves grown in vitro were used as initial explants. The explants were cultured in the MS media containing 3% sucrose, 0.7% agar at pH 5.8, five N6-benzylaminopurine (BAP) concentrations (0, 0.5, 1, 1.5 and 2 mg·1-1) and three indole-3-butyric acid (IBA) concentrations (0, 0.1 and 0.2 mg·1-1). The cultures were incubated at 21 ± 2 °C under photoperiod 16/8. After three subculturings (3 weeks-long each) the number of rhizome, shoots, buds, leaves, and roots, length of shoots and roots were recorded. Adventitious shoots formed directly on the leaf bases without callus intervention. Their number was affected by BAP concentrations. The highest shoots number, six per explants, was obtained at 1 mg·1-1 BAP and 0.1 mg·1-1 IBA. The shoot length decreased with the increasing concentration of BAP. The highest root number (2.7) was formed on shoots cultured on the MS medium containing 0.5 mg·1-1 α- naphthalene acetic acid, and the highest rhizome number (2.2) was formed on the medium with 0.5 mg·1-1 BAP. In vitro rooted plantlets were able to survive and acclimatize in the greenhouse. |
| first_indexed | 2024-12-13T15:41:33Z |
| format | Article |
| id | doaj.art-2c89a7a7a9bb4e48a15cdadd3f0ed2dd |
| institution | Directory Open Access Journal |
| issn | 2300-5009 |
| language | English |
| last_indexed | 2024-12-13T15:41:33Z |
| publishDate | 2013-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Horticultural Research |
| spelling | doaj.art-2c89a7a7a9bb4e48a15cdadd3f0ed2dd2022-12-21T23:39:49ZengSciendoJournal of Horticultural Research2300-50092013-12-01212233010.2478/johr-2013-0018Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf baseNasri Fardin0Mortazavi Seyed Najmedin1Ghaderi Nasser2Javadi Taimoor3Department of Horticultural Science, Agricultural Faculty, University of Kurdistan, IranDepartment of Horticultural Science, Agricultural Faculty, University of Zanjan, IranDepartment of Horticultural Science, Agricultural Faculty, University of Kurdistan, IranDepartment of Horticultural Science, Agricultural Faculty, University of Kurdistan, IranIn the present study, multiplication efficiency of Alstroemeria ligtu hybrid was investigated. Bases of the first seedling leaves grown in vitro were used as initial explants. The explants were cultured in the MS media containing 3% sucrose, 0.7% agar at pH 5.8, five N6-benzylaminopurine (BAP) concentrations (0, 0.5, 1, 1.5 and 2 mg·1-1) and three indole-3-butyric acid (IBA) concentrations (0, 0.1 and 0.2 mg·1-1). The cultures were incubated at 21 ± 2 °C under photoperiod 16/8. After three subculturings (3 weeks-long each) the number of rhizome, shoots, buds, leaves, and roots, length of shoots and roots were recorded. Adventitious shoots formed directly on the leaf bases without callus intervention. Their number was affected by BAP concentrations. The highest shoots number, six per explants, was obtained at 1 mg·1-1 BAP and 0.1 mg·1-1 IBA. The shoot length decreased with the increasing concentration of BAP. The highest root number (2.7) was formed on shoots cultured on the MS medium containing 0.5 mg·1-1 α- naphthalene acetic acid, and the highest rhizome number (2.2) was formed on the medium with 0.5 mg·1-1 BAP. In vitro rooted plantlets were able to survive and acclimatize in the greenhouse.https://doi.org/10.2478/johr-2013-0018alstroemeriadirect organogenesismicropropagation |
| spellingShingle | Nasri Fardin Mortazavi Seyed Najmedin Ghaderi Nasser Javadi Taimoor Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base Journal of Horticultural Research alstroemeria direct organogenesis micropropagation |
| title | Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base |
| title_full | Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base |
| title_fullStr | Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base |
| title_full_unstemmed | Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base |
| title_short | Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base |
| title_sort | propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base |
| topic | alstroemeria direct organogenesis micropropagation |
| url | https://doi.org/10.2478/johr-2013-0018 |
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