Determining the Synergistic Effect of Magnetic Fields on the Induction of Apoptosis by Folic Acid Targeted Iron Superparamagnetic Nanoparticles Loaded With Lomustine on U87-MG Cell Line
Background: Brain cancer is recognized as one of the deadliest cancers due to late detection and limitations of therapies. Glioblastoma occurs in different parts of the central nervous system and is the second leading cause of cancer death in people. There are many problems for the treatment of canc...
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| Format: | Article |
| Language: | English |
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Hormozgan University of Medical Sciences
2023-09-01
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| Series: | Disease and Diagnosis |
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| Online Access: | https://ddj.hums.ac.ir/PDF/ddj-12-161.pdf |
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| author | Zeinolabedin Shrifian Dastjerdi Elias Kargar Abargouei Fahimeh Zamani Rarani Ebrahim Eftekhar Mohammad Zamani Rarani Dariush Hooshyar Reza Afzalipour |
| author_facet | Zeinolabedin Shrifian Dastjerdi Elias Kargar Abargouei Fahimeh Zamani Rarani Ebrahim Eftekhar Mohammad Zamani Rarani Dariush Hooshyar Reza Afzalipour |
| author_sort | Zeinolabedin Shrifian Dastjerdi |
| collection | DOAJ |
| description | Background: Brain cancer is recognized as one of the deadliest cancers due to late detection and limitations of therapies. Glioblastoma occurs in different parts of the central nervous system and is the second leading cause of cancer death in people. There are many problems for the treatment of cancer cells. One of the limiting factors is the resistance of cancer cells to chemotherapy drugs. The use of nanoparticles (NPs) is an effective method for overcoming this problem. Materials and Methods: Fe3 O4 NPs were synthesized, and the size and morphology of NPs were determined by transmission electron microscopy, X-ray photoelectron spectroscopy, and Dynamic Light Scattering. The U87-MG cell line was cultured in Dulbecco’s modified Eagle medium and treated with nano, nano-lomustine, lomustine, and complex with/without magnetic fields. Finally, half maximal inhibitory concentration (IC50), MTT assay, and caspase8 and caspase9 expression were evaluated, and the data were analyzed with SPSS software. Results: Our results demonstrated that cell apoptosis increased in lomustine and complex groups, especially with the magnetic field (P>0.05). Based on caspase9 expression analysis, this rate was increased with the magnetic field vs. its absence (P>0.05). Conclusion: These findings indicated that a magnetic field, in addition to reducing the effective dose of lomustine, affects apoptosis with a change in the expression of genes involved in this process. |
| first_indexed | 2024-03-11T15:20:51Z |
| format | Article |
| id | doaj.art-2c89abc2a8e24bbeba95b399b8bbd339 |
| institution | Directory Open Access Journal |
| issn | 2717-3232 |
| language | English |
| last_indexed | 2024-03-11T15:20:51Z |
| publishDate | 2023-09-01 |
| publisher | Hormozgan University of Medical Sciences |
| record_format | Article |
| series | Disease and Diagnosis |
| spelling | doaj.art-2c89abc2a8e24bbeba95b399b8bbd3392023-10-28T08:27:16ZengHormozgan University of Medical SciencesDisease and Diagnosis2717-32322023-09-0112416116710.34172/ddj.2023.450ddj-450Determining the Synergistic Effect of Magnetic Fields on the Induction of Apoptosis by Folic Acid Targeted Iron Superparamagnetic Nanoparticles Loaded With Lomustine on U87-MG Cell LineZeinolabedin Shrifian Dastjerdi0Elias Kargar Abargouei1Fahimeh Zamani Rarani2Ebrahim Eftekhar3Mohammad Zamani Rarani4Dariush Hooshyar5Reza Afzalipour6Department of Anatomical Sciences, Medical School, Hormozgan University of Medical Sciences, Hormozgan, IranDepartment of Anatomical Sciences, Medical School, Hormozgan University of Medical Sciences, Hormozgan, IranDepartment of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranMolecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Science, Bandar Abbas, IranDepartment of Anatomical Sciences, Medical School, Hormozgan University of Medical Sciences, Hormozgan, IranDepartment of Anatomical Sciences, Medical School, Hormozgan University of Medical Sciences, Hormozgan, IranDepartment of Radiology, Faculty of Para-Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IranBackground: Brain cancer is recognized as one of the deadliest cancers due to late detection and limitations of therapies. Glioblastoma occurs in different parts of the central nervous system and is the second leading cause of cancer death in people. There are many problems for the treatment of cancer cells. One of the limiting factors is the resistance of cancer cells to chemotherapy drugs. The use of nanoparticles (NPs) is an effective method for overcoming this problem. Materials and Methods: Fe3 O4 NPs were synthesized, and the size and morphology of NPs were determined by transmission electron microscopy, X-ray photoelectron spectroscopy, and Dynamic Light Scattering. The U87-MG cell line was cultured in Dulbecco’s modified Eagle medium and treated with nano, nano-lomustine, lomustine, and complex with/without magnetic fields. Finally, half maximal inhibitory concentration (IC50), MTT assay, and caspase8 and caspase9 expression were evaluated, and the data were analyzed with SPSS software. Results: Our results demonstrated that cell apoptosis increased in lomustine and complex groups, especially with the magnetic field (P>0.05). Based on caspase9 expression analysis, this rate was increased with the magnetic field vs. its absence (P>0.05). Conclusion: These findings indicated that a magnetic field, in addition to reducing the effective dose of lomustine, affects apoptosis with a change in the expression of genes involved in this process.https://ddj.hums.ac.ir/PDF/ddj-12-161.pdfglioblastomananoparticleapoptosiscaspasemagnetic field |
| spellingShingle | Zeinolabedin Shrifian Dastjerdi Elias Kargar Abargouei Fahimeh Zamani Rarani Ebrahim Eftekhar Mohammad Zamani Rarani Dariush Hooshyar Reza Afzalipour Determining the Synergistic Effect of Magnetic Fields on the Induction of Apoptosis by Folic Acid Targeted Iron Superparamagnetic Nanoparticles Loaded With Lomustine on U87-MG Cell Line Disease and Diagnosis glioblastoma nanoparticle apoptosis caspase magnetic field |
| title | Determining the Synergistic Effect of Magnetic Fields on the Induction of Apoptosis by Folic Acid Targeted Iron Superparamagnetic Nanoparticles Loaded With Lomustine on U87-MG Cell Line |
| title_full | Determining the Synergistic Effect of Magnetic Fields on the Induction of Apoptosis by Folic Acid Targeted Iron Superparamagnetic Nanoparticles Loaded With Lomustine on U87-MG Cell Line |
| title_fullStr | Determining the Synergistic Effect of Magnetic Fields on the Induction of Apoptosis by Folic Acid Targeted Iron Superparamagnetic Nanoparticles Loaded With Lomustine on U87-MG Cell Line |
| title_full_unstemmed | Determining the Synergistic Effect of Magnetic Fields on the Induction of Apoptosis by Folic Acid Targeted Iron Superparamagnetic Nanoparticles Loaded With Lomustine on U87-MG Cell Line |
| title_short | Determining the Synergistic Effect of Magnetic Fields on the Induction of Apoptosis by Folic Acid Targeted Iron Superparamagnetic Nanoparticles Loaded With Lomustine on U87-MG Cell Line |
| title_sort | determining the synergistic effect of magnetic fields on the induction of apoptosis by folic acid targeted iron superparamagnetic nanoparticles loaded with lomustine on u87 mg cell line |
| topic | glioblastoma nanoparticle apoptosis caspase magnetic field |
| url | https://ddj.hums.ac.ir/PDF/ddj-12-161.pdf |
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