CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells
Abstract The CCCTC-binding factor (CTCF) protein and its modified forms regulate gene expression and genome organization. However, information on CTCF acetylation and its biological function is still lacking. Here, we show that CTCF can be acetylated at lysine 20 (CTCF-K20) by CREB-binding protein (...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-09-01
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| Series: | Cell Regeneration |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13619-022-00131-w |
| _version_ | 1828111712538066944 |
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| author | Shixin Gong Gongcheng Hu Rong Guo Jie Zhang Yiqi Yang Binrui Ji Gang Li Hongjie Yao |
| author_facet | Shixin Gong Gongcheng Hu Rong Guo Jie Zhang Yiqi Yang Binrui Ji Gang Li Hongjie Yao |
| author_sort | Shixin Gong |
| collection | DOAJ |
| description | Abstract The CCCTC-binding factor (CTCF) protein and its modified forms regulate gene expression and genome organization. However, information on CTCF acetylation and its biological function is still lacking. Here, we show that CTCF can be acetylated at lysine 20 (CTCF-K20) by CREB-binding protein (CBP) and deacetylated by histone deacetylase 6 (HDAC6). CTCF-K20 is required for the CTCF interaction with CBP. A CTCF point mutation at lysine 20 had no effect on self-renewal but blocked the mesoderm differentiation of mouse embryonic stem cells (mESCs). The CTCF-K20 mutation reduced CTCF binding to the promoters and enhancers of genes associated with early cardiac mesoderm differentiation, resulting in diminished chromatin accessibility and decreased enhancer-promoter interactions, impairing gene expression. In summary, this study reveals the important roles of CTCF-K20 in regulating CTCF genomic functions and mESC differentiation into mesoderm. |
| first_indexed | 2024-04-11T11:39:49Z |
| format | Article |
| id | doaj.art-2c8ab2d5a5604f80abfdf3b6e448f5ca |
| institution | Directory Open Access Journal |
| issn | 2045-9769 |
| language | English |
| last_indexed | 2024-04-11T11:39:49Z |
| publishDate | 2022-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Cell Regeneration |
| spelling | doaj.art-2c8ab2d5a5604f80abfdf3b6e448f5ca2022-12-22T04:25:51ZengSpringerOpenCell Regeneration2045-97692022-09-0111111710.1186/s13619-022-00131-wCTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cellsShixin Gong0Gongcheng Hu1Rong Guo2Jie Zhang3Yiqi Yang4Binrui Ji5Gang Li6Hongjie Yao7Center for Health Research, Joint School of Life Sciences, CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical UniversityCenter for Health Research, Joint School of Life Sciences, CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical UniversityCenter for Health Research, Joint School of Life Sciences, CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical UniversityCenter for Health Research, Joint School of Life Sciences, CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical UniversityCancer Centre, Faculty of Health Sciences, MoE Frontier Science Centre for Precision Oncology, University of MacauCenter for Health Research, Joint School of Life Sciences, CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical UniversityCancer Centre, Faculty of Health Sciences, MoE Frontier Science Centre for Precision Oncology, University of MacauCenter for Health Research, Joint School of Life Sciences, CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical UniversityAbstract The CCCTC-binding factor (CTCF) protein and its modified forms regulate gene expression and genome organization. However, information on CTCF acetylation and its biological function is still lacking. Here, we show that CTCF can be acetylated at lysine 20 (CTCF-K20) by CREB-binding protein (CBP) and deacetylated by histone deacetylase 6 (HDAC6). CTCF-K20 is required for the CTCF interaction with CBP. A CTCF point mutation at lysine 20 had no effect on self-renewal but blocked the mesoderm differentiation of mouse embryonic stem cells (mESCs). The CTCF-K20 mutation reduced CTCF binding to the promoters and enhancers of genes associated with early cardiac mesoderm differentiation, resulting in diminished chromatin accessibility and decreased enhancer-promoter interactions, impairing gene expression. In summary, this study reveals the important roles of CTCF-K20 in regulating CTCF genomic functions and mESC differentiation into mesoderm.https://doi.org/10.1186/s13619-022-00131-wCTCFCTCF acetylationCBPHDAC6Early cardiac mesoderm differentiation |
| spellingShingle | Shixin Gong Gongcheng Hu Rong Guo Jie Zhang Yiqi Yang Binrui Ji Gang Li Hongjie Yao CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells Cell Regeneration CTCF CTCF acetylation CBP HDAC6 Early cardiac mesoderm differentiation |
| title | CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
| title_full | CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
| title_fullStr | CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
| title_full_unstemmed | CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
| title_short | CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
| title_sort | ctcf acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
| topic | CTCF CTCF acetylation CBP HDAC6 Early cardiac mesoderm differentiation |
| url | https://doi.org/10.1186/s13619-022-00131-w |
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