Prevalence of indeterminate human immunodeficiency virus western blot results in pregnant women attended at a public hospital in Presidente Prudente, Brazil
The AIDS epidemic is spreading rapidly among women worldwide, offering increasing opportunities for vertical transmission of HIV. In Brazil, the prevalence of HIV infection among pregnant women is less than 1%. Therefore, the positive predictive value of an HIV EIA test tends to be lower than the mo...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
|
| Series: | Brazilian Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600009&lng=en&tlng=en |
| _version_ | 1819044502924427264 |
|---|---|
| author | Denise Cremonezi Paulo Eduardo de Mesquita Marisa Menezes Romão Luiz Euribel Prestes-Carneiro |
| author_facet | Denise Cremonezi Paulo Eduardo de Mesquita Marisa Menezes Romão Luiz Euribel Prestes-Carneiro |
| author_sort | Denise Cremonezi |
| collection | DOAJ |
| description | The AIDS epidemic is spreading rapidly among women worldwide, offering increasing opportunities for vertical transmission of HIV. In Brazil, the prevalence of HIV infection among pregnant women is less than 1%. Therefore, the positive predictive value of an HIV EIA test tends to be lower than the more frequent indeterminate Western blot result. Pregnant women receiving antenatal care, from 2000 to 2004, at a public secondary hospital in the city of Presidente Prudente, São Paulo, Brazil, were systematically screened for HIV by means of two distinct EIA tests, in order to determine the prevalence of indeterminate Western blot results among pregnant women showing discordance in both HIV EIA tests and indirect immunofluorescence assay. Confirmatory indirect immunofluorescence was performed on material for all women with positive results in both EIA tests. Whenever there were positive results in EIA and IIA, the applicant was retested by the initial screening assay. Only those not showing concordance in results in EIA and IAA had a Western blot performed. The viral load was measured in pregnant women with positive or indeterminate Western blot results. Out of 9,786 sera, 105 (1.0%) were positive in the two HIV EIA screening tests, confirmed by indirect immunofluorescence. Among these women, Western blot was interpreted as indeterminate in 11 (0.1%) cases and their viral load was <50 copies/mL. We found a prevalence of 0.1% HIV indeterminate Western blots in pregnant women from Presidente Prudente and the surrounding region; none of these pregnant women had positive HIV viral loads. |
| first_indexed | 2024-12-21T10:13:42Z |
| format | Article |
| id | doaj.art-2c8b4f406240465480dfb9889a5d36ce |
| institution | Directory Open Access Journal |
| issn | 1678-4391 |
| language | English |
| last_indexed | 2024-12-21T10:13:42Z |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-2c8b4f406240465480dfb9889a5d36ce2022-12-21T19:07:39ZengElsevierBrazilian Journal of Infectious Diseases1678-43919650650910.1590/S1413-86702005000600009S1413-86702005000600009Prevalence of indeterminate human immunodeficiency virus western blot results in pregnant women attended at a public hospital in Presidente Prudente, BrazilDenise Cremonezi0Paulo Eduardo de Mesquita1Marisa Menezes Romão2Luiz Euribel Prestes-Carneiro3Universidade do Oeste PaulistaUniversidade do Oeste PaulistaAdolfo Lutz InstituteUniversidade do Oeste PaulistaThe AIDS epidemic is spreading rapidly among women worldwide, offering increasing opportunities for vertical transmission of HIV. In Brazil, the prevalence of HIV infection among pregnant women is less than 1%. Therefore, the positive predictive value of an HIV EIA test tends to be lower than the more frequent indeterminate Western blot result. Pregnant women receiving antenatal care, from 2000 to 2004, at a public secondary hospital in the city of Presidente Prudente, São Paulo, Brazil, were systematically screened for HIV by means of two distinct EIA tests, in order to determine the prevalence of indeterminate Western blot results among pregnant women showing discordance in both HIV EIA tests and indirect immunofluorescence assay. Confirmatory indirect immunofluorescence was performed on material for all women with positive results in both EIA tests. Whenever there were positive results in EIA and IIA, the applicant was retested by the initial screening assay. Only those not showing concordance in results in EIA and IAA had a Western blot performed. The viral load was measured in pregnant women with positive or indeterminate Western blot results. Out of 9,786 sera, 105 (1.0%) were positive in the two HIV EIA screening tests, confirmed by indirect immunofluorescence. Among these women, Western blot was interpreted as indeterminate in 11 (0.1%) cases and their viral load was <50 copies/mL. We found a prevalence of 0.1% HIV indeterminate Western blots in pregnant women from Presidente Prudente and the surrounding region; none of these pregnant women had positive HIV viral loads.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600009&lng=en&tlng=enHIV-1indeterminate Western blotpregnant women |
| spellingShingle | Denise Cremonezi Paulo Eduardo de Mesquita Marisa Menezes Romão Luiz Euribel Prestes-Carneiro Prevalence of indeterminate human immunodeficiency virus western blot results in pregnant women attended at a public hospital in Presidente Prudente, Brazil Brazilian Journal of Infectious Diseases HIV-1 indeterminate Western blot pregnant women |
| title | Prevalence of indeterminate human immunodeficiency virus western blot results in pregnant women attended at a public hospital in Presidente Prudente, Brazil |
| title_full | Prevalence of indeterminate human immunodeficiency virus western blot results in pregnant women attended at a public hospital in Presidente Prudente, Brazil |
| title_fullStr | Prevalence of indeterminate human immunodeficiency virus western blot results in pregnant women attended at a public hospital in Presidente Prudente, Brazil |
| title_full_unstemmed | Prevalence of indeterminate human immunodeficiency virus western blot results in pregnant women attended at a public hospital in Presidente Prudente, Brazil |
| title_short | Prevalence of indeterminate human immunodeficiency virus western blot results in pregnant women attended at a public hospital in Presidente Prudente, Brazil |
| title_sort | prevalence of indeterminate human immunodeficiency virus western blot results in pregnant women attended at a public hospital in presidente prudente brazil |
| topic | HIV-1 indeterminate Western blot pregnant women |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600009&lng=en&tlng=en |
| work_keys_str_mv | AT denisecremonezi prevalenceofindeterminatehumanimmunodeficiencyviruswesternblotresultsinpregnantwomenattendedatapublichospitalinpresidenteprudentebrazil AT pauloeduardodemesquita prevalenceofindeterminatehumanimmunodeficiencyviruswesternblotresultsinpregnantwomenattendedatapublichospitalinpresidenteprudentebrazil AT marisamenezesromao prevalenceofindeterminatehumanimmunodeficiencyviruswesternblotresultsinpregnantwomenattendedatapublichospitalinpresidenteprudentebrazil AT luizeuribelprestescarneiro prevalenceofindeterminatehumanimmunodeficiencyviruswesternblotresultsinpregnantwomenattendedatapublichospitalinpresidenteprudentebrazil |