High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity
<p>Abstract</p> <p>Background</p> <p>Relatively little is known about the role of specific saturated fatty acids in the development of high fat diet induced obesity and insulin resistance. Here, we have studied the effect of stearate in high fat diets (45% energy as fat...
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| Format: | Article |
| Language: | English |
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BMC
2010-03-01
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| Series: | Nutrition & Metabolism |
| Online Access: | http://www.nutritionandmetabolism.com/content/7/1/24 |
| _version_ | 1818484652943343616 |
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| author | Havekes Louis M Frants Rune R Voshol Peter J Ouwens Margriet Bijland Silvia Guigas Bruno van den Berg Sjoerd AA Romijn Johannes A van Dijk Ko |
| author_facet | Havekes Louis M Frants Rune R Voshol Peter J Ouwens Margriet Bijland Silvia Guigas Bruno van den Berg Sjoerd AA Romijn Johannes A van Dijk Ko |
| author_sort | Havekes Louis M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Relatively little is known about the role of specific saturated fatty acids in the development of high fat diet induced obesity and insulin resistance. Here, we have studied the effect of stearate in high fat diets (45% energy as fat) on whole body energy metabolism and tissue specific insulin sensitivity.</p> <p>Methods</p> <p>C57Bl/6 mice were fed a low stearate diet based on palm oil or one of two stearate rich diets, one diet based on lard and one diet based on palm oil supplemented with tristearin (to the stearate level of the lard based diet), for a period of 5 weeks. <it>Ad libitum </it>fed Oxidative metabolism was assessed by indirect calorimetry at week 5. Changes in body mass and composition was assessed by DEXA scan analysis. Tissue specific insulin sensitivity was assessed by hyperinsulinemic-euglycemic clamp analysis and Western blot at the end of week 5.</p> <p>Results</p> <p>Indirect calorimetry analysis revealed that high levels of dietary stearate resulted in lower caloric energy expenditure characterized by lower oxidation of fatty acids. In agreement with this metabolic phenotype, mice on the stearate rich diets gained more adipose tissue mass. Whole body and tissue specific insulin sensitivity was assessed by hyperinsulinemic-euglycemic clamp and analysis of insulin induced PKB<sup>ser473 </sup>phosphorylation. Whole body insulin sensitivity was decreased by all high fat diets. However, while insulin-stimulated glucose uptake by peripheral tissues was impaired by all high fat diets, hepatic insulin sensitivity was affected only by the stearate rich diets. This tissue-specific pattern of reduced insulin sensitivity was confirmed by similar impairment in insulin-induced phosphorylation of PKB<sup>ser473 </sup>in both liver and skeletal muscle.</p> <p>Conclusion</p> <p>In C57Bl/6 mice, 5 weeks of a high fat diet rich in stearate induces a metabolic state favoring low oxidative metabolism, increased adiposity and whole body insulin resistance characterized by severe hepatic insulin resistance. These results indicate that dietary fatty acid composition <it>per sé </it>rather than dietary fat content determines insulin sensitivity in liver of high fat fed C57Bl/6 mice.</p> |
| first_indexed | 2024-12-10T15:57:57Z |
| format | Article |
| id | doaj.art-2c8c448ab45b4d9c99d1bb67b88466a2 |
| institution | Directory Open Access Journal |
| issn | 1743-7075 |
| language | English |
| last_indexed | 2024-12-10T15:57:57Z |
| publishDate | 2010-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Nutrition & Metabolism |
| spelling | doaj.art-2c8c448ab45b4d9c99d1bb67b88466a22022-12-22T01:42:33ZengBMCNutrition & Metabolism1743-70752010-03-01712410.1186/1743-7075-7-24High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivityHavekes Louis MFrants Rune RVoshol Peter JOuwens MargrietBijland SilviaGuigas Brunovan den Berg Sjoerd AARomijn Johannes Avan Dijk Ko<p>Abstract</p> <p>Background</p> <p>Relatively little is known about the role of specific saturated fatty acids in the development of high fat diet induced obesity and insulin resistance. Here, we have studied the effect of stearate in high fat diets (45% energy as fat) on whole body energy metabolism and tissue specific insulin sensitivity.</p> <p>Methods</p> <p>C57Bl/6 mice were fed a low stearate diet based on palm oil or one of two stearate rich diets, one diet based on lard and one diet based on palm oil supplemented with tristearin (to the stearate level of the lard based diet), for a period of 5 weeks. <it>Ad libitum </it>fed Oxidative metabolism was assessed by indirect calorimetry at week 5. Changes in body mass and composition was assessed by DEXA scan analysis. Tissue specific insulin sensitivity was assessed by hyperinsulinemic-euglycemic clamp analysis and Western blot at the end of week 5.</p> <p>Results</p> <p>Indirect calorimetry analysis revealed that high levels of dietary stearate resulted in lower caloric energy expenditure characterized by lower oxidation of fatty acids. In agreement with this metabolic phenotype, mice on the stearate rich diets gained more adipose tissue mass. Whole body and tissue specific insulin sensitivity was assessed by hyperinsulinemic-euglycemic clamp and analysis of insulin induced PKB<sup>ser473 </sup>phosphorylation. Whole body insulin sensitivity was decreased by all high fat diets. However, while insulin-stimulated glucose uptake by peripheral tissues was impaired by all high fat diets, hepatic insulin sensitivity was affected only by the stearate rich diets. This tissue-specific pattern of reduced insulin sensitivity was confirmed by similar impairment in insulin-induced phosphorylation of PKB<sup>ser473 </sup>in both liver and skeletal muscle.</p> <p>Conclusion</p> <p>In C57Bl/6 mice, 5 weeks of a high fat diet rich in stearate induces a metabolic state favoring low oxidative metabolism, increased adiposity and whole body insulin resistance characterized by severe hepatic insulin resistance. These results indicate that dietary fatty acid composition <it>per sé </it>rather than dietary fat content determines insulin sensitivity in liver of high fat fed C57Bl/6 mice.</p>http://www.nutritionandmetabolism.com/content/7/1/24 |
| spellingShingle | Havekes Louis M Frants Rune R Voshol Peter J Ouwens Margriet Bijland Silvia Guigas Bruno van den Berg Sjoerd AA Romijn Johannes A van Dijk Ko High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity Nutrition & Metabolism |
| title | High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity |
| title_full | High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity |
| title_fullStr | High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity |
| title_full_unstemmed | High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity |
| title_short | High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity |
| title_sort | high levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity |
| url | http://www.nutritionandmetabolism.com/content/7/1/24 |
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