Biocompatibility and biofilm formation on conventional and CAD/CAM provisional implant restorations
Abstract Dental implant treatment is a complex and sophisticated process, and implant provisional restorations play a vital role in ensuring its success. The advent of computer-aided design and computer-aided manufacturing (CAD/CAM) technology has revolutionized the field of implant restorations by...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-023-03468-z |
| _version_ | 1797556009377464320 |
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| author | Tipparat Parakaw Nisarat Ruangsawasdi Pornpen Dararat Chareerut Phruksaniyom Sirada Srihirun Pobploy Petchmedyai |
| author_facet | Tipparat Parakaw Nisarat Ruangsawasdi Pornpen Dararat Chareerut Phruksaniyom Sirada Srihirun Pobploy Petchmedyai |
| author_sort | Tipparat Parakaw |
| collection | DOAJ |
| description | Abstract Dental implant treatment is a complex and sophisticated process, and implant provisional restorations play a vital role in ensuring its success. The advent of computer-aided design and computer-aided manufacturing (CAD/CAM) technology has revolutionized the field of implant restorations by providing improved precision leading to a reduction in chair time and more predictable treatment outcomes. This technology offers a promising solution to the drawbacks of conventional methods and has the potential to transform the way implant procedures are approached. Despite the clear advantages of CAD/CAM over conventional provisional implant restorations including higher accuracy of fit and superior mechanical properties, little research has been conducted on the biological aspect of these novel restorations. This study aims to fill that gap, comprehensively assessing the biocompatibility, gingival tissue attachment and biofilm formation of a range of provisional implant restorations using CAD/CAM technology through milling and 3-D printing processes compared to conventional fabrication. The biocompatibility of the tested restorations was assessed by MTT assay, Calcein-AM assay as well as SEM analysis. The surface roughness of the tested samples was evaluated, alongside the attachment of Human Gingival Fibroblasts (HGF) cells as well as biofilm formation, and estimated Porphyromonas gingivalis (P. gingivalis) cell count from DNA detection. The results showed all tested provisional implant restorations were non-toxic and good HGF cell attachment but differed in their quantity of biofilm formation, with surface texture influenced by the material and fabrication technique, playing a role. Within the limitation of this study, the findings suggest that CAD/CAM-fabricated provisional implant restorations using a milling technique may be the most favourable among tested groups in terms of biocompatibility and periodontal-related biofilm formation. |
| first_indexed | 2024-03-10T16:56:37Z |
| format | Article |
| id | doaj.art-2c90802b0229483ca27af3481c7848ac |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-03-10T16:56:37Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-2c90802b0229483ca27af3481c7848ac2023-11-20T11:07:19ZengBMCBMC Oral Health1472-68312023-10-0123111510.1186/s12903-023-03468-zBiocompatibility and biofilm formation on conventional and CAD/CAM provisional implant restorationsTipparat Parakaw0Nisarat Ruangsawasdi1Pornpen Dararat2Chareerut Phruksaniyom3Sirada Srihirun4Pobploy Petchmedyai5Department of Pharmacology, Faculty of Dentistry, Mahidol UniversityDepartment of Pharmacology, Faculty of Dentistry, Mahidol UniversityDepartment of Pharmacology, Faculty of Dentistry, Mahidol UniversityDepartment of Pharmacology, Faculty of Dentistry, Mahidol UniversityDepartment of Pharmacology, Faculty of Dentistry, Mahidol UniversityDental Implant Center, Faculty of Dentistry, Mahidol UniversityAbstract Dental implant treatment is a complex and sophisticated process, and implant provisional restorations play a vital role in ensuring its success. The advent of computer-aided design and computer-aided manufacturing (CAD/CAM) technology has revolutionized the field of implant restorations by providing improved precision leading to a reduction in chair time and more predictable treatment outcomes. This technology offers a promising solution to the drawbacks of conventional methods and has the potential to transform the way implant procedures are approached. Despite the clear advantages of CAD/CAM over conventional provisional implant restorations including higher accuracy of fit and superior mechanical properties, little research has been conducted on the biological aspect of these novel restorations. This study aims to fill that gap, comprehensively assessing the biocompatibility, gingival tissue attachment and biofilm formation of a range of provisional implant restorations using CAD/CAM technology through milling and 3-D printing processes compared to conventional fabrication. The biocompatibility of the tested restorations was assessed by MTT assay, Calcein-AM assay as well as SEM analysis. The surface roughness of the tested samples was evaluated, alongside the attachment of Human Gingival Fibroblasts (HGF) cells as well as biofilm formation, and estimated Porphyromonas gingivalis (P. gingivalis) cell count from DNA detection. The results showed all tested provisional implant restorations were non-toxic and good HGF cell attachment but differed in their quantity of biofilm formation, with surface texture influenced by the material and fabrication technique, playing a role. Within the limitation of this study, the findings suggest that CAD/CAM-fabricated provisional implant restorations using a milling technique may be the most favourable among tested groups in terms of biocompatibility and periodontal-related biofilm formation.https://doi.org/10.1186/s12903-023-03468-zDental prosthesisComputer-aided manufacturingPorphyromonas gingivalisFibroblastCytotoxicity tests |
| spellingShingle | Tipparat Parakaw Nisarat Ruangsawasdi Pornpen Dararat Chareerut Phruksaniyom Sirada Srihirun Pobploy Petchmedyai Biocompatibility and biofilm formation on conventional and CAD/CAM provisional implant restorations BMC Oral Health Dental prosthesis Computer-aided manufacturing Porphyromonas gingivalis Fibroblast Cytotoxicity tests |
| title | Biocompatibility and biofilm formation on conventional and CAD/CAM provisional implant restorations |
| title_full | Biocompatibility and biofilm formation on conventional and CAD/CAM provisional implant restorations |
| title_fullStr | Biocompatibility and biofilm formation on conventional and CAD/CAM provisional implant restorations |
| title_full_unstemmed | Biocompatibility and biofilm formation on conventional and CAD/CAM provisional implant restorations |
| title_short | Biocompatibility and biofilm formation on conventional and CAD/CAM provisional implant restorations |
| title_sort | biocompatibility and biofilm formation on conventional and cad cam provisional implant restorations |
| topic | Dental prosthesis Computer-aided manufacturing Porphyromonas gingivalis Fibroblast Cytotoxicity tests |
| url | https://doi.org/10.1186/s12903-023-03468-z |
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