Molecular detection of cestoda in black francolin (Francolinus francolinus) of Babylon Province, Iraq
Cestodes or tapeworms (Class Cestoda, phylum Platyhelminthes) are widespread parasites of birds, as they cause various damages, which may lead to the death of their hosts, especially when the infection is severe. The Black Francolin belongs to the Phasianidae family of pheasants and, in the order Ga...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2023-04-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_177328_518dd7f9280895c2a4b8afc14993e45c.pdf |
| _version_ | 1827975380218150912 |
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| author | Ameer I. Alanad Afaq T. Farhood |
| author_facet | Ameer I. Alanad Afaq T. Farhood |
| author_sort | Ameer I. Alanad |
| collection | DOAJ |
| description | Cestodes or tapeworms (Class Cestoda, phylum Platyhelminthes) are widespread parasites of birds, as they cause various damages, which may lead to the death of their hosts, especially when the infection is severe. The Black Francolin belongs to the Phasianidae family of pheasants and, in the order Galliformes, the gallinaceous birds, and it is called <em>Francolinus francolinus</em>. Considering it as a local bird in Asia, it was referred to as the black partridge. The current study was conducted from January to October 2019 to determine the Cestoda infections within <em>F. francolinus</em>. Thirty-five birds from <em>F. francolinus</em> were examined for the presence of cestoda, of which 17 birds (48.57%) were infected with cestoda parasites. Molecular diagnosis based on sequences of cytochrome c oxidase 1 (COI) gene regions was performed to confirm the diagnosis of this cestoda. This revealed a close identification of up to 93.44-100% for COI gene regions with other cestoda species obtained from GenBank. Phylogenetic analysis supported the placement of this species within three families, Davaineidae, Taeniidae, and Hymenolepididae, with close relationships to the previously described species of <em>Raillietina</em> sp., <em>R. mahnerti</em>, <em>R. hymenolepidoides</em>, <em>Ophryocotyloides dasi</em>, <em>Rodentolepis nana</em> and <em>Hydatigera parva</em> based on the COI gene regions. This is the first study in Iraq to diagnose Cestoda in birds by molecular methods, where five species of cestoda parasitic on <em>F. francolinus</em> were recorded for the first time in Iraq. |
| first_indexed | 2024-04-09T20:10:40Z |
| format | Article |
| id | doaj.art-2c91440dcc5349c984f94d6b874b9515 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-04-09T20:10:40Z |
| publishDate | 2023-04-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-2c91440dcc5349c984f94d6b874b95152023-03-31T23:07:07ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552023-04-0137241942310.33899/ijvs.2022.134858.2412177328Molecular detection of cestoda in black francolin (Francolinus francolinus) of Babylon Province, IraqAmeer I. Alanad0Afaq T. Farhood1Department of Science, College of Basic Education, University of Babylon, Babylon, IraqDepartment of Pathological Analysis, College of Science, University of Thi-Qar, Thi-Qar, IraqCestodes or tapeworms (Class Cestoda, phylum Platyhelminthes) are widespread parasites of birds, as they cause various damages, which may lead to the death of their hosts, especially when the infection is severe. The Black Francolin belongs to the Phasianidae family of pheasants and, in the order Galliformes, the gallinaceous birds, and it is called <em>Francolinus francolinus</em>. Considering it as a local bird in Asia, it was referred to as the black partridge. The current study was conducted from January to October 2019 to determine the Cestoda infections within <em>F. francolinus</em>. Thirty-five birds from <em>F. francolinus</em> were examined for the presence of cestoda, of which 17 birds (48.57%) were infected with cestoda parasites. Molecular diagnosis based on sequences of cytochrome c oxidase 1 (COI) gene regions was performed to confirm the diagnosis of this cestoda. This revealed a close identification of up to 93.44-100% for COI gene regions with other cestoda species obtained from GenBank. Phylogenetic analysis supported the placement of this species within three families, Davaineidae, Taeniidae, and Hymenolepididae, with close relationships to the previously described species of <em>Raillietina</em> sp., <em>R. mahnerti</em>, <em>R. hymenolepidoides</em>, <em>Ophryocotyloides dasi</em>, <em>Rodentolepis nana</em> and <em>Hydatigera parva</em> based on the COI gene regions. This is the first study in Iraq to diagnose Cestoda in birds by molecular methods, where five species of cestoda parasitic on <em>F. francolinus</em> were recorded for the first time in Iraq.https://www.vetmedmosul.com/article_177328_518dd7f9280895c2a4b8afc14993e45c.pdfblack francolinnested-pcrtapeworm |
| spellingShingle | Ameer I. Alanad Afaq T. Farhood Molecular detection of cestoda in black francolin (Francolinus francolinus) of Babylon Province, Iraq Iraqi Journal of Veterinary Sciences black francolin nested-pcr tapeworm |
| title | Molecular detection of cestoda in black francolin (Francolinus francolinus) of Babylon Province, Iraq |
| title_full | Molecular detection of cestoda in black francolin (Francolinus francolinus) of Babylon Province, Iraq |
| title_fullStr | Molecular detection of cestoda in black francolin (Francolinus francolinus) of Babylon Province, Iraq |
| title_full_unstemmed | Molecular detection of cestoda in black francolin (Francolinus francolinus) of Babylon Province, Iraq |
| title_short | Molecular detection of cestoda in black francolin (Francolinus francolinus) of Babylon Province, Iraq |
| title_sort | molecular detection of cestoda in black francolin francolinus francolinus of babylon province iraq |
| topic | black francolin nested-pcr tapeworm |
| url | https://www.vetmedmosul.com/article_177328_518dd7f9280895c2a4b8afc14993e45c.pdf |
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