Ceftaroline fosamil laden allograft: A new modality in reducing infection?
Purpose: Allograft infection remains the greatest challenge in orthopaedic reconstructive surgery especially methicillin-resistant Staphylococcus aureus (MRSA). This risk can be minimized with the use of antibiotic laden allograft (ALA) via iontophoresis. Ceftaroline fosamil (CF) is an advanced-gene...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2018-10-01
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| Series: | Journal of Orthopaedic Surgery |
| Online Access: | https://doi.org/10.1177/2309499018806671 |
| _version_ | 1818554426148782080 |
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| author | Vivek Ajit Singh Lim Han Sim Amber Haseeb Cindy Teh Shuan Ju |
| author_facet | Vivek Ajit Singh Lim Han Sim Amber Haseeb Cindy Teh Shuan Ju |
| author_sort | Vivek Ajit Singh |
| collection | DOAJ |
| description | Purpose: Allograft infection remains the greatest challenge in orthopaedic reconstructive surgery especially methicillin-resistant Staphylococcus aureus (MRSA). This risk can be minimized with the use of antibiotic laden allograft (ALA) via iontophoresis. Ceftaroline fosamil (CF) is an advanced-generation cephalosporin, an alternative treatment for MRSA infections. Its antibacterial activity and safety profile are better than vancomycin. CF iontophoresed bone has not been used before. This study was conducted to establish the feasibility of creating a CF ALA and establish the prime conditions for its expenditure. Method: We created an iontophoresis cell; 3% CF was inserted within medullary segment of goat bone and sealed from external saline solution. The cell operated at the following voltages 30, 60 and 90 V and at the following durations 5, 10, 15, 20, 25 and 30 min. Information regarding optimal conditions for its application was then obtained. After which, correlation between voltages and time with CF concentration in the bone was analysed. A bioavailability test was also conducted to observe the optimal rate of CF elution from the graft. Result: The optimal condition for the impregnation process is 3% CF at 90 V for 10 min. Bone graft impregnated with CF at optimal conditions can elute above minimum inhibitory concentration of the CF against MRSA for 21 days. Conclusion: CF iontophoresis was found feasible for allograft impregnation. The technique is simple, inexpensive and reproducible clinically. Iontophoresis offers a novel solution to reduce the rate of perioperative infection in reconstructive surgery involving use of bone graft. |
| first_indexed | 2024-12-12T09:39:20Z |
| format | Article |
| id | doaj.art-2c965d2f6876482aafff7a888edfbefe |
| institution | Directory Open Access Journal |
| issn | 2309-4990 |
| language | English |
| last_indexed | 2024-12-12T09:39:20Z |
| publishDate | 2018-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Orthopaedic Surgery |
| spelling | doaj.art-2c965d2f6876482aafff7a888edfbefe2022-12-22T00:28:38ZengSAGE PublishingJournal of Orthopaedic Surgery2309-49902018-10-012610.1177/2309499018806671Ceftaroline fosamil laden allograft: A new modality in reducing infection?Vivek Ajit Singh0Lim Han Sim1Amber Haseeb2Cindy Teh Shuan Ju3 Department of Orthopedics, University of Malaya, Kuala Lumpur, Malaysia Department of Orthopedics, University of Malaya, Kuala Lumpur, Malaysia Department of Orthopedics, University of Malaya, Kuala Lumpur, Malaysia Department of Microbiology, University of Malaya, Kuala Lumpur, MalaysiaPurpose: Allograft infection remains the greatest challenge in orthopaedic reconstructive surgery especially methicillin-resistant Staphylococcus aureus (MRSA). This risk can be minimized with the use of antibiotic laden allograft (ALA) via iontophoresis. Ceftaroline fosamil (CF) is an advanced-generation cephalosporin, an alternative treatment for MRSA infections. Its antibacterial activity and safety profile are better than vancomycin. CF iontophoresed bone has not been used before. This study was conducted to establish the feasibility of creating a CF ALA and establish the prime conditions for its expenditure. Method: We created an iontophoresis cell; 3% CF was inserted within medullary segment of goat bone and sealed from external saline solution. The cell operated at the following voltages 30, 60 and 90 V and at the following durations 5, 10, 15, 20, 25 and 30 min. Information regarding optimal conditions for its application was then obtained. After which, correlation between voltages and time with CF concentration in the bone was analysed. A bioavailability test was also conducted to observe the optimal rate of CF elution from the graft. Result: The optimal condition for the impregnation process is 3% CF at 90 V for 10 min. Bone graft impregnated with CF at optimal conditions can elute above minimum inhibitory concentration of the CF against MRSA for 21 days. Conclusion: CF iontophoresis was found feasible for allograft impregnation. The technique is simple, inexpensive and reproducible clinically. Iontophoresis offers a novel solution to reduce the rate of perioperative infection in reconstructive surgery involving use of bone graft.https://doi.org/10.1177/2309499018806671 |
| spellingShingle | Vivek Ajit Singh Lim Han Sim Amber Haseeb Cindy Teh Shuan Ju Ceftaroline fosamil laden allograft: A new modality in reducing infection? Journal of Orthopaedic Surgery |
| title | Ceftaroline fosamil laden allograft: A new modality in reducing infection? |
| title_full | Ceftaroline fosamil laden allograft: A new modality in reducing infection? |
| title_fullStr | Ceftaroline fosamil laden allograft: A new modality in reducing infection? |
| title_full_unstemmed | Ceftaroline fosamil laden allograft: A new modality in reducing infection? |
| title_short | Ceftaroline fosamil laden allograft: A new modality in reducing infection? |
| title_sort | ceftaroline fosamil laden allograft a new modality in reducing infection |
| url | https://doi.org/10.1177/2309499018806671 |
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