Study of the Contents of Analogues of Aristolochic Acid in <i>Houttuynia cordata</i> by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry
In this study, a new and simple method was established for the simultaneous determination of analogues of aristolochic acids (aristolochic acid I, aristolochic acid II, aristolactam I and aristolactam AII) in <i>Houttuynia cordata</i> by ultra-high performance liquid chromatography–quadr...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/2304-8158/11/3/302 |
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| author | Xuan Yu Yuan Gao Ying Xu Xian Guo Lan Guo Ting Tan Fan Liu Yiqun Wan |
| author_facet | Xuan Yu Yuan Gao Ying Xu Xian Guo Lan Guo Ting Tan Fan Liu Yiqun Wan |
| author_sort | Xuan Yu |
| collection | DOAJ |
| description | In this study, a new and simple method was established for the simultaneous determination of analogues of aristolochic acids (aristolochic acid I, aristolochic acid II, aristolactam I and aristolactam AII) in <i>Houttuynia cordata</i> by ultra-high performance liquid chromatography–quadrupole/time-of-flight mass spectrometry (UHPLC–Q/TOF-MS). The samples were ultrasonically extracted with methanol, purified with HC-C18, and then separated on a C18 column (75 × 2.1 mm, 2.0 μm) at 35 °C. Under the optimized conditions, aristolochic acid I (AA-I), aristolochic acid II (AA-II), aristolactam I (AL-I) and aristolactam AII (AL-AII) all showed good linear regression (not less than 0.9987). The average recoveries of the four analytes were within the range of 72.3–105.5%, with the relative standard deviations (RSDs) being ≤7.6%. The proposed method was then applied to the determination of <i>Houttuynia cordata</i> samples collected from different regions in China. The results showed that none of the three carcinogenic substances (aristolochic acid I, aristolochic acid II and aristolactam I) were detected in any of the 22 samples collected from 22 different regions of China, while aristolactam AII, which has not been reported to have genotoxicity, was detected in all samples. This study provides a valuable reference for the further safety assessment of <i>Houttuynia cordata</i>. |
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| last_indexed | 2024-03-09T23:53:18Z |
| publishDate | 2022-01-01 |
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| series | Foods |
| spelling | doaj.art-2c96ca224ebc4adba12aa54a5589ed9f2023-11-23T16:28:39ZengMDPI AGFoods2304-81582022-01-0111330210.3390/foods11030302Study of the Contents of Analogues of Aristolochic Acid in <i>Houttuynia cordata</i> by Ultra-High Performance Liquid Chromatography Tandem Mass SpectrometryXuan Yu0Yuan Gao1Ying Xu2Xian Guo3Lan Guo4Ting Tan5Fan Liu6Yiqun Wan7College of Chemistry, Nanchang University, Nanchang 330047, ChinaCollege of Chemistry, Nanchang University, Nanchang 330047, ChinaCollege of Chemistry, Nanchang University, Nanchang 330047, ChinaCollege of Chemistry, Nanchang University, Nanchang 330047, ChinaCollege of Chemistry, Nanchang University, Nanchang 330047, ChinaJiangxi Province Key Laboratory of Modern Analytical Science, Nanchang University, Nanchang 330047, ChinaJiangxi Province Key Laboratory of Modern Analytical Science, Nanchang University, Nanchang 330047, ChinaCollege of Chemistry, Nanchang University, Nanchang 330047, ChinaIn this study, a new and simple method was established for the simultaneous determination of analogues of aristolochic acids (aristolochic acid I, aristolochic acid II, aristolactam I and aristolactam AII) in <i>Houttuynia cordata</i> by ultra-high performance liquid chromatography–quadrupole/time-of-flight mass spectrometry (UHPLC–Q/TOF-MS). The samples were ultrasonically extracted with methanol, purified with HC-C18, and then separated on a C18 column (75 × 2.1 mm, 2.0 μm) at 35 °C. Under the optimized conditions, aristolochic acid I (AA-I), aristolochic acid II (AA-II), aristolactam I (AL-I) and aristolactam AII (AL-AII) all showed good linear regression (not less than 0.9987). The average recoveries of the four analytes were within the range of 72.3–105.5%, with the relative standard deviations (RSDs) being ≤7.6%. The proposed method was then applied to the determination of <i>Houttuynia cordata</i> samples collected from different regions in China. The results showed that none of the three carcinogenic substances (aristolochic acid I, aristolochic acid II and aristolactam I) were detected in any of the 22 samples collected from 22 different regions of China, while aristolactam AII, which has not been reported to have genotoxicity, was detected in all samples. This study provides a valuable reference for the further safety assessment of <i>Houttuynia cordata</i>.https://www.mdpi.com/2304-8158/11/3/302<i>Houttuynia cordata</i>aristolochic acid analoguesdetectionUHPLC-Q/TOF-MS |
| spellingShingle | Xuan Yu Yuan Gao Ying Xu Xian Guo Lan Guo Ting Tan Fan Liu Yiqun Wan Study of the Contents of Analogues of Aristolochic Acid in <i>Houttuynia cordata</i> by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry Foods <i>Houttuynia cordata</i> aristolochic acid analogues detection UHPLC-Q/TOF-MS |
| title | Study of the Contents of Analogues of Aristolochic Acid in <i>Houttuynia cordata</i> by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry |
| title_full | Study of the Contents of Analogues of Aristolochic Acid in <i>Houttuynia cordata</i> by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry |
| title_fullStr | Study of the Contents of Analogues of Aristolochic Acid in <i>Houttuynia cordata</i> by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry |
| title_full_unstemmed | Study of the Contents of Analogues of Aristolochic Acid in <i>Houttuynia cordata</i> by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry |
| title_short | Study of the Contents of Analogues of Aristolochic Acid in <i>Houttuynia cordata</i> by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry |
| title_sort | study of the contents of analogues of aristolochic acid in i houttuynia cordata i by ultra high performance liquid chromatography tandem mass spectrometry |
| topic | <i>Houttuynia cordata</i> aristolochic acid analogues detection UHPLC-Q/TOF-MS |
| url | https://www.mdpi.com/2304-8158/11/3/302 |
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