Relation of the disaccharidases in the small intestine of the rat to the degree of experimentally induced iron-deficiency anemia
Hypolactasia associated with severe iron-deficiency anemia has been reported in several studies. The objective of the present study was to determine whether hypolactasia is associated with the degree and duration of iron-deficiency anemia. Newly weaned male Wistar rats were divided into a control gr...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2000-05-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500008 |
| _version_ | 1817997998632730624 |
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| author | M.R. Vieira L.C. Galvão M.I.M. Fernandes |
| author_facet | M.R. Vieira L.C. Galvão M.I.M. Fernandes |
| author_sort | M.R. Vieira |
| collection | DOAJ |
| description | Hypolactasia associated with severe iron-deficiency anemia has been reported in several studies. The objective of the present study was to determine whether hypolactasia is associated with the degree and duration of iron-deficiency anemia. Newly weaned male Wistar rats were divided into a control group receiving a diet supplemented with iron (C) and an experimental group (E) receiving a diet not supplemented with iron (iron-deficiency diet). The animals were studied on the 3rd, 5th, 7th, 14th, 21st, 28th and 35th days of the experiment, when overall and iron nutritional status and disaccharidase activity in the small intestine were determined by the Dahlqvist method. A reduction in weight occurred in the anemic animals starting on the 5th day of the study. Anemia was present in the experimental animals, with a progressive worsening up to the 14th day (hemoglobin: C = 13.27 and E = 5.37) and stabilizing thereafter. Saccharase and maltase activities did not differ significantly between groups, whereas lactase showed a significant reduction in total (TA) and specific activity (SA) in the anemic animals starting on the 21st day of the study. Median lactase TA for the C and E groups was 2.27 and 1.25 U on the 21st day, 2.87 and 1.88 U on the 28th day, and 4.20 and 1.59 U on the 35th day, respectively. Median lactase SA was 0.31 and 0.20 U/g wet weight on the 21st day, 0.39 and 0.24 U/g wet weight on the 28th day, and 0.42 and 0.23 U/g wet weight on the 35th day, respectively. These findings suggest a relationship between the enzymatic alterations observed and both the degree and duration of the anemic process. Analysis of other studies on intestinal disaccharidases in anemia suggests that the mechanism of these changes may be functional, i.e., that the enterocytes may suffer a reduction in their ability to synthesize these enzymes. |
| first_indexed | 2024-04-14T02:46:34Z |
| format | Article |
| id | doaj.art-2c97cfcaf04a4871bcc29d9014076986 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-14T02:46:34Z |
| publishDate | 2000-05-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-2c97cfcaf04a4871bcc29d90140769862022-12-22T02:16:28ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2000-05-0133553954410.1590/S0100-879X2000000500008Relation of the disaccharidases in the small intestine of the rat to the degree of experimentally induced iron-deficiency anemiaM.R. VieiraL.C. GalvãoM.I.M. FernandesHypolactasia associated with severe iron-deficiency anemia has been reported in several studies. The objective of the present study was to determine whether hypolactasia is associated with the degree and duration of iron-deficiency anemia. Newly weaned male Wistar rats were divided into a control group receiving a diet supplemented with iron (C) and an experimental group (E) receiving a diet not supplemented with iron (iron-deficiency diet). The animals were studied on the 3rd, 5th, 7th, 14th, 21st, 28th and 35th days of the experiment, when overall and iron nutritional status and disaccharidase activity in the small intestine were determined by the Dahlqvist method. A reduction in weight occurred in the anemic animals starting on the 5th day of the study. Anemia was present in the experimental animals, with a progressive worsening up to the 14th day (hemoglobin: C = 13.27 and E = 5.37) and stabilizing thereafter. Saccharase and maltase activities did not differ significantly between groups, whereas lactase showed a significant reduction in total (TA) and specific activity (SA) in the anemic animals starting on the 21st day of the study. Median lactase TA for the C and E groups was 2.27 and 1.25 U on the 21st day, 2.87 and 1.88 U on the 28th day, and 4.20 and 1.59 U on the 35th day, respectively. Median lactase SA was 0.31 and 0.20 U/g wet weight on the 21st day, 0.39 and 0.24 U/g wet weight on the 28th day, and 0.42 and 0.23 U/g wet weight on the 35th day, respectively. These findings suggest a relationship between the enzymatic alterations observed and both the degree and duration of the anemic process. Analysis of other studies on intestinal disaccharidases in anemia suggests that the mechanism of these changes may be functional, i.e., that the enterocytes may suffer a reduction in their ability to synthesize these enzymes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500008disaccharidaseslactaseanemiairon deficiencyintestinal mucosa |
| spellingShingle | M.R. Vieira L.C. Galvão M.I.M. Fernandes Relation of the disaccharidases in the small intestine of the rat to the degree of experimentally induced iron-deficiency anemia Brazilian Journal of Medical and Biological Research disaccharidases lactase anemia iron deficiency intestinal mucosa |
| title | Relation of the disaccharidases in the small intestine of the rat to the degree of experimentally induced iron-deficiency anemia |
| title_full | Relation of the disaccharidases in the small intestine of the rat to the degree of experimentally induced iron-deficiency anemia |
| title_fullStr | Relation of the disaccharidases in the small intestine of the rat to the degree of experimentally induced iron-deficiency anemia |
| title_full_unstemmed | Relation of the disaccharidases in the small intestine of the rat to the degree of experimentally induced iron-deficiency anemia |
| title_short | Relation of the disaccharidases in the small intestine of the rat to the degree of experimentally induced iron-deficiency anemia |
| title_sort | relation of the disaccharidases in the small intestine of the rat to the degree of experimentally induced iron deficiency anemia |
| topic | disaccharidases lactase anemia iron deficiency intestinal mucosa |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500008 |
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