Phytochemical profiling, antimicrobial, and antioxidant activities of hydroethanolic extracts of prickly pear (Opuntia ficus indica) fruit and pulp
Abstract Phenolic compounds in prickly pear [Opuntia ficus indica (L.) Mill.] are known to contribute to the antioxidant and antimicrobial activities of the prickly pear. The present study aimed to evaluate the antioxidants and in vitro antimicrobial potential in the hydroethanolic extracts of diffe...
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| Format: | Article |
| Language: | English |
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Wiley
2023-04-01
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| Series: | Food Science & Nutrition |
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| Online Access: | https://doi.org/10.1002/fsn3.3226 |
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| author | Khansa Iftikhar Farzana Siddique Kashif Ameer Muhammad Arshad Sadia Kharal Isam A. Mohamed Ahmed Zarina Yasmin Nida Aziz |
| author_facet | Khansa Iftikhar Farzana Siddique Kashif Ameer Muhammad Arshad Sadia Kharal Isam A. Mohamed Ahmed Zarina Yasmin Nida Aziz |
| author_sort | Khansa Iftikhar |
| collection | DOAJ |
| description | Abstract Phenolic compounds in prickly pear [Opuntia ficus indica (L.) Mill.] are known to contribute to the antioxidant and antimicrobial activities of the prickly pear. The present study aimed to evaluate the antioxidants and in vitro antimicrobial potential in the hydroethanolic extracts of different parts (fruit, cladode, and pulp) of prickly pear. Different polyphenolic compounds were analyzed by using high‐performance liquid chromatography. The results indicated that cladode possessed a higher quantity of phenolics compared with that observed in fruit and pulp. The most important phenolic compound in high quantity was gallic acid (66.19 μg/g) in cladode. The 100% aqueous extract of cladode exhibited the highest antioxidant (92%) and antimicrobial activities against Salmonella typhi (3.40 mg/ml), Helicobacter pylori (1.37 mg/ml), Escherichia coli (1.41 mg/ml), and Staphylococcus aureus (1.41 mg/ml). Principal component analysis (PCA) indicated that antioxidant activity and minimum inhibitory concentration (MIC) responses had a significant negative correlation with each other. Overall, the current results provided basic data for choosing prickly pear cladode with high antioxidant capacity for the development and consumption of antioxidant‐based alternative medicines and value addition of formulated foods. |
| first_indexed | 2024-04-09T18:42:37Z |
| format | Article |
| id | doaj.art-2c9a139b4bc442158448d3d0be403311 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-04-09T18:42:37Z |
| publishDate | 2023-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-2c9a139b4bc442158448d3d0be4033112023-04-10T17:14:36ZengWileyFood Science & Nutrition2048-71772023-04-011141916193010.1002/fsn3.3226Phytochemical profiling, antimicrobial, and antioxidant activities of hydroethanolic extracts of prickly pear (Opuntia ficus indica) fruit and pulpKhansa Iftikhar0Farzana Siddique1Kashif Ameer2Muhammad Arshad3Sadia Kharal4Isam A. Mohamed Ahmed5Zarina Yasmin6Nida Aziz7Institute of Food Science and Nutrition, University of Sargodha Sargodha PakistanInstitute of Food Science and Nutrition, University of Sargodha Sargodha PakistanInstitute of Food Science and Nutrition, University of Sargodha Sargodha PakistanDepartment of Zoology University of Sargodha Sargodha Punjab PakistanInstitute of Food Science and Nutrition, University of Sargodha Sargodha PakistanDepartment of Food Science and Technology, Faculty of Agriculture University of Khartoum Shambat SudanPost Harvest Research Centre Ayub Agricultural Research Institute Faisalabad PakistanDepartment of Zoology University of Punjab Lahore PakistanAbstract Phenolic compounds in prickly pear [Opuntia ficus indica (L.) Mill.] are known to contribute to the antioxidant and antimicrobial activities of the prickly pear. The present study aimed to evaluate the antioxidants and in vitro antimicrobial potential in the hydroethanolic extracts of different parts (fruit, cladode, and pulp) of prickly pear. Different polyphenolic compounds were analyzed by using high‐performance liquid chromatography. The results indicated that cladode possessed a higher quantity of phenolics compared with that observed in fruit and pulp. The most important phenolic compound in high quantity was gallic acid (66.19 μg/g) in cladode. The 100% aqueous extract of cladode exhibited the highest antioxidant (92%) and antimicrobial activities against Salmonella typhi (3.40 mg/ml), Helicobacter pylori (1.37 mg/ml), Escherichia coli (1.41 mg/ml), and Staphylococcus aureus (1.41 mg/ml). Principal component analysis (PCA) indicated that antioxidant activity and minimum inhibitory concentration (MIC) responses had a significant negative correlation with each other. Overall, the current results provided basic data for choosing prickly pear cladode with high antioxidant capacity for the development and consumption of antioxidant‐based alternative medicines and value addition of formulated foods.https://doi.org/10.1002/fsn3.3226antioxidantscladodeflavonoidsOpuntiapolyphenols |
| spellingShingle | Khansa Iftikhar Farzana Siddique Kashif Ameer Muhammad Arshad Sadia Kharal Isam A. Mohamed Ahmed Zarina Yasmin Nida Aziz Phytochemical profiling, antimicrobial, and antioxidant activities of hydroethanolic extracts of prickly pear (Opuntia ficus indica) fruit and pulp Food Science & Nutrition antioxidants cladode flavonoids Opuntia polyphenols |
| title | Phytochemical profiling, antimicrobial, and antioxidant activities of hydroethanolic extracts of prickly pear (Opuntia ficus indica) fruit and pulp |
| title_full | Phytochemical profiling, antimicrobial, and antioxidant activities of hydroethanolic extracts of prickly pear (Opuntia ficus indica) fruit and pulp |
| title_fullStr | Phytochemical profiling, antimicrobial, and antioxidant activities of hydroethanolic extracts of prickly pear (Opuntia ficus indica) fruit and pulp |
| title_full_unstemmed | Phytochemical profiling, antimicrobial, and antioxidant activities of hydroethanolic extracts of prickly pear (Opuntia ficus indica) fruit and pulp |
| title_short | Phytochemical profiling, antimicrobial, and antioxidant activities of hydroethanolic extracts of prickly pear (Opuntia ficus indica) fruit and pulp |
| title_sort | phytochemical profiling antimicrobial and antioxidant activities of hydroethanolic extracts of prickly pear opuntia ficus indica fruit and pulp |
| topic | antioxidants cladode flavonoids Opuntia polyphenols |
| url | https://doi.org/10.1002/fsn3.3226 |
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