Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion
Abstract Background Obesity is a major public health concern worldwide. A sedentary life and a nutritional transition to processed foods and high-calorie diets are contributing factors to obesity. The demand for nutraceutical foods, such as herbal weight-loss products, which offer the potential to c...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-02-01
|
| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-020-2824-x |
| _version_ | 1818971143147618304 |
|---|---|
| author | Cécile Héliès-Toussaint Edwin Fouché Nathalie Naud Florence Blas-Y-Estrada Maria del Socorro Santos-Diaz Anne Nègre-Salvayre Ana Paulina Barba de la Rosa Françoise Guéraud |
| author_facet | Cécile Héliès-Toussaint Edwin Fouché Nathalie Naud Florence Blas-Y-Estrada Maria del Socorro Santos-Diaz Anne Nègre-Salvayre Ana Paulina Barba de la Rosa Françoise Guéraud |
| author_sort | Cécile Héliès-Toussaint |
| collection | DOAJ |
| description | Abstract Background Obesity is a major public health concern worldwide. A sedentary life and a nutritional transition to processed foods and high-calorie diets are contributing factors to obesity. The demand for nutraceutical foods, such as herbal weight-loss products, which offer the potential to counteract obesity, has consequently increased. We hypothesised that Opuntia cladodes consumption could assist weight management in an obesity prevention context. Methods This study was designed to explore the anti-adipogenic effects of lyophilised Opuntia cladode powders (OCP) in an in vitro cellular model for adipocyte differentiation and an in vivo high-fat-diet (HFD)-induced obesity rat model. Two OCP were tested, one from wild species O. streptacantha and the second from the most known species O. ficus-indica. Results Pre-adipocytes 3 T3-F442A were treated by OCP during the differentiation process by insulin. OCP treatment impaired the differentiation in adipocytes, as supported by the decreased triglyceride content and a low glucose uptake, which remained comparable to that observed in undifferentiated controls, suggesting that an anti-adipogenic effect was exerted by OCP. Sprague–Dawley rats were fed with a normal or HFD, supplemented or not with OCP for 8 weeks. OCP treatment slightly reduced body weight gain, liver and abdominal fat weights, improved some obesity-related metabolic parameters and increased triglyceride excretion in the faeces. Taken together, these results showed that OCP might contribute to reduce adipogenesis and fat storage in a HFD context, notably by promoting the faecal excretion of fats. Conclusions Opuntia cladodes may be used as a dietary supplement or potential therapeutic agent in diet-based therapies for weight management to prevent obesity. Graphical abstract |
| first_indexed | 2024-12-20T14:47:41Z |
| format | Article |
| id | doaj.art-2c9a7a10751e470ab82db6d9231ccd0f |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-20T14:47:41Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-2c9a7a10751e470ab82db6d9231ccd0f2022-12-21T19:37:04ZengBMCBMC Complementary Medicine and Therapies2662-76712020-02-0120111210.1186/s12906-020-2824-xOpuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretionCécile Héliès-Toussaint0Edwin Fouché1Nathalie Naud2Florence Blas-Y-Estrada3Maria del Socorro Santos-Diaz4Anne Nègre-Salvayre5Ana Paulina Barba de la Rosa6Françoise Guéraud7INRA, ToxAlim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPSINRA, ToxAlim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPSINRA, ToxAlim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPSINRA, ToxAlim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPSCentro de Investigación y Estudios de Posgrado (CienciasQuímicas), UniversidadAutónoma de San Luis PotosíInstituto Potosino de Investigación Científica y TecnológicaINSERM U1048, Institute of Metabolic and Cardiovascular Diseases I2MCINRA, ToxAlim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPSAbstract Background Obesity is a major public health concern worldwide. A sedentary life and a nutritional transition to processed foods and high-calorie diets are contributing factors to obesity. The demand for nutraceutical foods, such as herbal weight-loss products, which offer the potential to counteract obesity, has consequently increased. We hypothesised that Opuntia cladodes consumption could assist weight management in an obesity prevention context. Methods This study was designed to explore the anti-adipogenic effects of lyophilised Opuntia cladode powders (OCP) in an in vitro cellular model for adipocyte differentiation and an in vivo high-fat-diet (HFD)-induced obesity rat model. Two OCP were tested, one from wild species O. streptacantha and the second from the most known species O. ficus-indica. Results Pre-adipocytes 3 T3-F442A were treated by OCP during the differentiation process by insulin. OCP treatment impaired the differentiation in adipocytes, as supported by the decreased triglyceride content and a low glucose uptake, which remained comparable to that observed in undifferentiated controls, suggesting that an anti-adipogenic effect was exerted by OCP. Sprague–Dawley rats were fed with a normal or HFD, supplemented or not with OCP for 8 weeks. OCP treatment slightly reduced body weight gain, liver and abdominal fat weights, improved some obesity-related metabolic parameters and increased triglyceride excretion in the faeces. Taken together, these results showed that OCP might contribute to reduce adipogenesis and fat storage in a HFD context, notably by promoting the faecal excretion of fats. Conclusions Opuntia cladodes may be used as a dietary supplement or potential therapeutic agent in diet-based therapies for weight management to prevent obesity. Graphical abstracthttps://doi.org/10.1186/s12906-020-2824-xOpuntiaAnti-obesity3T3-F442A adipocytesRat high-fat dietFaecal fat excretion |
| spellingShingle | Cécile Héliès-Toussaint Edwin Fouché Nathalie Naud Florence Blas-Y-Estrada Maria del Socorro Santos-Diaz Anne Nègre-Salvayre Ana Paulina Barba de la Rosa Françoise Guéraud Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion BMC Complementary Medicine and Therapies Opuntia Anti-obesity 3T3-F442A adipocytes Rat high-fat diet Faecal fat excretion |
| title | Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion |
| title_full | Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion |
| title_fullStr | Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion |
| title_full_unstemmed | Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion |
| title_short | Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion |
| title_sort | opuntia cladode powders inhibit adipogenesis in 3 t3 f442a adipocytes and a high fat diet rat model by modifying metabolic parameters and favouring faecal fat excretion |
| topic | Opuntia Anti-obesity 3T3-F442A adipocytes Rat high-fat diet Faecal fat excretion |
| url | https://doi.org/10.1186/s12906-020-2824-x |
| work_keys_str_mv | AT cecileheliestoussaint opuntiacladodepowdersinhibitadipogenesisin3t3f442aadipocytesandahighfatdietratmodelbymodifyingmetabolicparametersandfavouringfaecalfatexcretion AT edwinfouche opuntiacladodepowdersinhibitadipogenesisin3t3f442aadipocytesandahighfatdietratmodelbymodifyingmetabolicparametersandfavouringfaecalfatexcretion AT nathalienaud opuntiacladodepowdersinhibitadipogenesisin3t3f442aadipocytesandahighfatdietratmodelbymodifyingmetabolicparametersandfavouringfaecalfatexcretion AT florenceblasyestrada opuntiacladodepowdersinhibitadipogenesisin3t3f442aadipocytesandahighfatdietratmodelbymodifyingmetabolicparametersandfavouringfaecalfatexcretion AT mariadelsocorrosantosdiaz opuntiacladodepowdersinhibitadipogenesisin3t3f442aadipocytesandahighfatdietratmodelbymodifyingmetabolicparametersandfavouringfaecalfatexcretion AT annenegresalvayre opuntiacladodepowdersinhibitadipogenesisin3t3f442aadipocytesandahighfatdietratmodelbymodifyingmetabolicparametersandfavouringfaecalfatexcretion AT anapaulinabarbadelarosa opuntiacladodepowdersinhibitadipogenesisin3t3f442aadipocytesandahighfatdietratmodelbymodifyingmetabolicparametersandfavouringfaecalfatexcretion AT francoisegueraud opuntiacladodepowdersinhibitadipogenesisin3t3f442aadipocytesandahighfatdietratmodelbymodifyingmetabolicparametersandfavouringfaecalfatexcretion |