The Potential Role of CERS1 in Autophagy Through PI3K/AKT Signaling Pathway in Hypophysoma
To explore the role and mechanism of CERS1 in hypophysoma and investigate whether CERS1 overexpression can change the autophagy process of hypophysoma, and then to explore whether CERS1’s effect was regulated by the PI3K/AKT signaling pathway. Western blot and RT-PCR were used to analyze the express...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2020-12-01
|
| Series: | Technology in Cancer Research & Treatment |
| Online Access: | https://doi.org/10.1177/1533033820977536 |
| _version_ | 1818389356738510848 |
|---|---|
| author | Jingtao Wang MM Jimin Zhang MM Dongzhou Ma MM Xiushan Li MB |
| author_facet | Jingtao Wang MM Jimin Zhang MM Dongzhou Ma MM Xiushan Li MB |
| author_sort | Jingtao Wang MM |
| collection | DOAJ |
| description | To explore the role and mechanism of CERS1 in hypophysoma and investigate whether CERS1 overexpression can change the autophagy process of hypophysoma, and then to explore whether CERS1’s effect was regulated by the PI3K/AKT signaling pathway. Western blot and RT-PCR were used to analyze the expression or mRNA level of CERS1 at different tissues or cell lines. Afterwards, the occurrence and development of hypophysoma in vivo and in vitro, respectively, was observed by using CERS1 overexpression by lentivirus. Finally, MK-2206 and LY294002 were applied to discuss whether the role of CERS1 was regulated by the PI3K/AKT signaling pathway. Results show that the CERS1 expression and mRNA level in tumor or AtT-20 cells were decreased. CERS1 over-expressed by lentivirus could inhibit hypophysoma development in vivo and in vitro by reducing tumor volume and weight, weakening tumor proliferation and invasion, and enhancing apoptosis. In addition, shCERS1 could reverse the process. The above results indicate that CERS1 is possibly able to enhance autophagy in hypophysoma through the PI3K/AKT signaling pathway. |
| first_indexed | 2024-12-14T04:40:26Z |
| format | Article |
| id | doaj.art-2c9b01dd10dc4ed28f8866e0a2ccbe6f |
| institution | Directory Open Access Journal |
| issn | 1533-0338 |
| language | English |
| last_indexed | 2024-12-14T04:40:26Z |
| publishDate | 2020-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Technology in Cancer Research & Treatment |
| spelling | doaj.art-2c9b01dd10dc4ed28f8866e0a2ccbe6f2022-12-21T23:16:48ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382020-12-011910.1177/1533033820977536The Potential Role of CERS1 in Autophagy Through PI3K/AKT Signaling Pathway in HypophysomaJingtao Wang MM0Jimin Zhang MM1Dongzhou Ma MM2Xiushan Li MB3 Third Department of Neurosurgery, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, People’s Republic of China Third Department of Neurosurgery, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, People’s Republic of China Third Department of Neurosurgery, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, People’s Republic of China Third Department of Neurosurgery, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, People’s Republic of ChinaTo explore the role and mechanism of CERS1 in hypophysoma and investigate whether CERS1 overexpression can change the autophagy process of hypophysoma, and then to explore whether CERS1’s effect was regulated by the PI3K/AKT signaling pathway. Western blot and RT-PCR were used to analyze the expression or mRNA level of CERS1 at different tissues or cell lines. Afterwards, the occurrence and development of hypophysoma in vivo and in vitro, respectively, was observed by using CERS1 overexpression by lentivirus. Finally, MK-2206 and LY294002 were applied to discuss whether the role of CERS1 was regulated by the PI3K/AKT signaling pathway. Results show that the CERS1 expression and mRNA level in tumor or AtT-20 cells were decreased. CERS1 over-expressed by lentivirus could inhibit hypophysoma development in vivo and in vitro by reducing tumor volume and weight, weakening tumor proliferation and invasion, and enhancing apoptosis. In addition, shCERS1 could reverse the process. The above results indicate that CERS1 is possibly able to enhance autophagy in hypophysoma through the PI3K/AKT signaling pathway.https://doi.org/10.1177/1533033820977536 |
| spellingShingle | Jingtao Wang MM Jimin Zhang MM Dongzhou Ma MM Xiushan Li MB The Potential Role of CERS1 in Autophagy Through PI3K/AKT Signaling Pathway in Hypophysoma Technology in Cancer Research & Treatment |
| title | The Potential Role of CERS1 in Autophagy Through PI3K/AKT Signaling Pathway in Hypophysoma |
| title_full | The Potential Role of CERS1 in Autophagy Through PI3K/AKT Signaling Pathway in Hypophysoma |
| title_fullStr | The Potential Role of CERS1 in Autophagy Through PI3K/AKT Signaling Pathway in Hypophysoma |
| title_full_unstemmed | The Potential Role of CERS1 in Autophagy Through PI3K/AKT Signaling Pathway in Hypophysoma |
| title_short | The Potential Role of CERS1 in Autophagy Through PI3K/AKT Signaling Pathway in Hypophysoma |
| title_sort | potential role of cers1 in autophagy through pi3k akt signaling pathway in hypophysoma |
| url | https://doi.org/10.1177/1533033820977536 |
| work_keys_str_mv | AT jingtaowangmm thepotentialroleofcers1inautophagythroughpi3kaktsignalingpathwayinhypophysoma AT jiminzhangmm thepotentialroleofcers1inautophagythroughpi3kaktsignalingpathwayinhypophysoma AT dongzhoumamm thepotentialroleofcers1inautophagythroughpi3kaktsignalingpathwayinhypophysoma AT xiushanlimb thepotentialroleofcers1inautophagythroughpi3kaktsignalingpathwayinhypophysoma AT jingtaowangmm potentialroleofcers1inautophagythroughpi3kaktsignalingpathwayinhypophysoma AT jiminzhangmm potentialroleofcers1inautophagythroughpi3kaktsignalingpathwayinhypophysoma AT dongzhoumamm potentialroleofcers1inautophagythroughpi3kaktsignalingpathwayinhypophysoma AT xiushanlimb potentialroleofcers1inautophagythroughpi3kaktsignalingpathwayinhypophysoma |