Solubilization of Na,K-ATPase from rabbit kidney outer medulla using only C12E8

Solubilization of Na,K-ATPase from rabbit kidney outer medulla using only C12E8

SDS, C12E8, CHAPS or CHAPSO or a combination of two of these detergents is generally used for the solubilization of Na,K-ATPase and other ATPases. Our method using only C12E8 has the advantage of considerable reduction of the time for enzyme purification, with rapid solubilization and purification i...

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Bibliographic Details
Main Authors: H.L. Santos, R.P. Lamas, P. Ciancaglini
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2002-03-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Na,K-ATPase
Rabbit kidney medulla
Membrane solubilization
C12E8, (alphaß)2 Dimer
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300002

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