The protective effect of Er-Xian decoction against myocardial injury in menopausal rat model
Abstract Background Er-Xian decoction (EXD), a formula of Chinese medicine, is often used to treat menopausal syndrome in China. The aim of the present study was to explore the potential cardioprotective mechanism of EXD against myocardial injury in an ovariectomy-induced menopausal rat model. Metho...
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2311-9 |
| _version_ | 1818498771187662848 |
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| author | Zhiguo Zhang Lihua Xiang Lanping Zhao Hong Jiao Zhen Wang Yubo Li Yanjing Chen |
| author_facet | Zhiguo Zhang Lihua Xiang Lanping Zhao Hong Jiao Zhen Wang Yubo Li Yanjing Chen |
| author_sort | Zhiguo Zhang |
| collection | DOAJ |
| description | Abstract Background Er-Xian decoction (EXD), a formula of Chinese medicine, is often used to treat menopausal syndrome in China. The aim of the present study was to explore the potential cardioprotective mechanism of EXD against myocardial injury in an ovariectomy-induced menopausal rat model. Methods We divided the female Wistar rats into ovariectomy group and sham operation group (SHAM group). The ovariectomized (OVX) rats received treatment of vehicle (OVX group), EXD (EXD group) or 17β-estradiol (E2 group). After 12-week of treatment, the level of estradiol in serum was detected using an electrochemiluminescence immunoassay, and electrophysiologic changes in myocardial action potentials (AP) were evaluated using intracellular microelectrode technique. Changes in the histopathology of the left ventricle and the ultrastructure of the cardiomyocytes were observed by hematoxylin and eosin (HE) staining and transmission electronmicroscopy to assess myocardial injury. Microarrays were applied for the evaluation of gene expression profiles in ventricular muscle of the OVX and EXD rats. Further pathway analyses of the differential expression genes were carried out using the Kyoto Encyclopedia of Genes and Genomes (KEGG). And real-time quantitative RT-PCR (qRT-PCR) was used for verification of the key findings. Results The results from electrophysiological and histomorphological observations demonstrated that EXD had a substantial myocardial protective effect. The EXD-treated rats, in comparison with the OVX rats, demonstrated up-regulated expression of 28 genes yet down-regulated expression of 157 genes in the ventricular muscle. The qRT-PCR assay validated all selected differential expression genes. The KEGG pathway analysis showed that the down-regulated genes were relevant to cardiomyopathy and myocardial contractility. EXD could decrease the mRNA expressions of cardiac myosin (Myh7, Myl2) and integrin (Itgb5) in the ventricular myocardium. Conclusion EXD had a protective effect against myocardial injury in OVX rats, and this cardioprotective effect may be associated with modulation of the expression of cardiac myosin or integrin at the mRNA level. |
| first_indexed | 2024-12-10T20:20:09Z |
| format | Article |
| id | doaj.art-2c9b65e2e0c74b8cbcac39db7a379376 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-10T20:20:09Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-2c9b65e2e0c74b8cbcac39db7a3793762022-12-22T01:35:04ZengBMCBMC Complementary and Alternative Medicine1472-68822018-09-0118111010.1186/s12906-018-2311-9The protective effect of Er-Xian decoction against myocardial injury in menopausal rat modelZhiguo Zhang0Lihua Xiang1Lanping Zhao2Hong Jiao3Zhen Wang4Yubo Li5Yanjing Chen6Institute of Basic Theory, China Academy of Chinese Medical SciencesInstitute of Basic Theory, China Academy of Chinese Medical SciencesBasic Medical College, Hebei North UniversityBasic Medical College, Hebei North UniversityInstitute of Basic Theory, China Academy of Chinese Medical SciencesInstitute of Basic Theory, China Academy of Chinese Medical SciencesInstitute of Basic Theory, China Academy of Chinese Medical SciencesAbstract Background Er-Xian decoction (EXD), a formula of Chinese medicine, is often used to treat menopausal syndrome in China. The aim of the present study was to explore the potential cardioprotective mechanism of EXD against myocardial injury in an ovariectomy-induced menopausal rat model. Methods We divided the female Wistar rats into ovariectomy group and sham operation group (SHAM group). The ovariectomized (OVX) rats received treatment of vehicle (OVX group), EXD (EXD group) or 17β-estradiol (E2 group). After 12-week of treatment, the level of estradiol in serum was detected using an electrochemiluminescence immunoassay, and electrophysiologic changes in myocardial action potentials (AP) were evaluated using intracellular microelectrode technique. Changes in the histopathology of the left ventricle and the ultrastructure of the cardiomyocytes were observed by hematoxylin and eosin (HE) staining and transmission electronmicroscopy to assess myocardial injury. Microarrays were applied for the evaluation of gene expression profiles in ventricular muscle of the OVX and EXD rats. Further pathway analyses of the differential expression genes were carried out using the Kyoto Encyclopedia of Genes and Genomes (KEGG). And real-time quantitative RT-PCR (qRT-PCR) was used for verification of the key findings. Results The results from electrophysiological and histomorphological observations demonstrated that EXD had a substantial myocardial protective effect. The EXD-treated rats, in comparison with the OVX rats, demonstrated up-regulated expression of 28 genes yet down-regulated expression of 157 genes in the ventricular muscle. The qRT-PCR assay validated all selected differential expression genes. The KEGG pathway analysis showed that the down-regulated genes were relevant to cardiomyopathy and myocardial contractility. EXD could decrease the mRNA expressions of cardiac myosin (Myh7, Myl2) and integrin (Itgb5) in the ventricular myocardium. Conclusion EXD had a protective effect against myocardial injury in OVX rats, and this cardioprotective effect may be associated with modulation of the expression of cardiac myosin or integrin at the mRNA level.http://link.springer.com/article/10.1186/s12906-018-2311-9Er-Xian decoctionCardioprotective effectCardiomyopathyMenopauseOvariectomy |
| spellingShingle | Zhiguo Zhang Lihua Xiang Lanping Zhao Hong Jiao Zhen Wang Yubo Li Yanjing Chen The protective effect of Er-Xian decoction against myocardial injury in menopausal rat model BMC Complementary and Alternative Medicine Er-Xian decoction Cardioprotective effect Cardiomyopathy Menopause Ovariectomy |
| title | The protective effect of Er-Xian decoction against myocardial injury in menopausal rat model |
| title_full | The protective effect of Er-Xian decoction against myocardial injury in menopausal rat model |
| title_fullStr | The protective effect of Er-Xian decoction against myocardial injury in menopausal rat model |
| title_full_unstemmed | The protective effect of Er-Xian decoction against myocardial injury in menopausal rat model |
| title_short | The protective effect of Er-Xian decoction against myocardial injury in menopausal rat model |
| title_sort | protective effect of er xian decoction against myocardial injury in menopausal rat model |
| topic | Er-Xian decoction Cardioprotective effect Cardiomyopathy Menopause Ovariectomy |
| url | http://link.springer.com/article/10.1186/s12906-018-2311-9 |
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