Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology?
Recent findings call for the critical overview of some incorrectly used plant cell and tissue culture terminology such as dedifferentiation, callus, totipotency, and somatic embryogenesis. Plant cell and tissue culture methods are efficient means to preserve and propagate genotypes with superior ger...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-04-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00536/full |
| _version_ | 1818302415166767104 |
|---|---|
| author | Attila Fehér Attila Fehér |
| author_facet | Attila Fehér Attila Fehér |
| author_sort | Attila Fehér |
| collection | DOAJ |
| description | Recent findings call for the critical overview of some incorrectly used plant cell and tissue culture terminology such as dedifferentiation, callus, totipotency, and somatic embryogenesis. Plant cell and tissue culture methods are efficient means to preserve and propagate genotypes with superior germplasm as well as to increase genetic variability for breading. Besides, they are useful research tools and objects of plant developmental biology. The history of plant cell and tissue culture dates back to more than a century. Its basic methodology and terminology were formulated preceding modern plant biology. Recent progress in molecular and cell biology techniques allowed unprecedented insights into the underlying processes of plant cell/tissue culture and regeneration. The main aim of this review is to provide a theoretical framework supported by recent experimental findings to reconsider certain historical, even dogmatic, statements widely used by plant scientists and teachers such as “plant cells are totipotent” or “callus is a mass of dedifferentiated cells,” or “somatic embryos have a single cell origin.” These statements are based on a confused terminology. Clarification of it might help to avoid further misunderstanding and to overcome potential “terminology-raised” barriers in plant research. |
| first_indexed | 2024-12-13T05:38:32Z |
| format | Article |
| id | doaj.art-2c9d703c138143b3bc8ead499816e76e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T05:38:32Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2c9d703c138143b3bc8ead499816e76e2022-12-21T23:57:50ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-04-011010.3389/fpls.2019.00536442509Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology?Attila Fehér0Attila Fehér1Department of Plant Biology, University of Szeged, Szeged, HungaryInstitute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Szeged, HungaryRecent findings call for the critical overview of some incorrectly used plant cell and tissue culture terminology such as dedifferentiation, callus, totipotency, and somatic embryogenesis. Plant cell and tissue culture methods are efficient means to preserve and propagate genotypes with superior germplasm as well as to increase genetic variability for breading. Besides, they are useful research tools and objects of plant developmental biology. The history of plant cell and tissue culture dates back to more than a century. Its basic methodology and terminology were formulated preceding modern plant biology. Recent progress in molecular and cell biology techniques allowed unprecedented insights into the underlying processes of plant cell/tissue culture and regeneration. The main aim of this review is to provide a theoretical framework supported by recent experimental findings to reconsider certain historical, even dogmatic, statements widely used by plant scientists and teachers such as “plant cells are totipotent” or “callus is a mass of dedifferentiated cells,” or “somatic embryos have a single cell origin.” These statements are based on a confused terminology. Clarification of it might help to avoid further misunderstanding and to overcome potential “terminology-raised” barriers in plant research.https://www.frontiersin.org/article/10.3389/fpls.2019.00536/fullcallusdedifferentiationplant regenerationplant cell and tissue culturesomatic embryogenesisterminology |
| spellingShingle | Attila Fehér Attila Fehér Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology? Frontiers in Plant Science callus dedifferentiation plant regeneration plant cell and tissue culture somatic embryogenesis terminology |
| title | Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology? |
| title_full | Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology? |
| title_fullStr | Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology? |
| title_full_unstemmed | Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology? |
| title_short | Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology? |
| title_sort | callus dedifferentiation totipotency somatic embryogenesis what these terms mean in the era of molecular plant biology |
| topic | callus dedifferentiation plant regeneration plant cell and tissue culture somatic embryogenesis terminology |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00536/full |
| work_keys_str_mv | AT attilafeher callusdedifferentiationtotipotencysomaticembryogenesiswhatthesetermsmeanintheeraofmolecularplantbiology AT attilafeher callusdedifferentiationtotipotencysomaticembryogenesiswhatthesetermsmeanintheeraofmolecularplantbiology |