Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organisms
This paper presents material and technological studies on lab-on-chip (LOC) devices as a first step towards biocompatible and reliable research on microscopic fungi and soil organisms on a microscale. This approach is intended to respond to the growing need for environmental control and protection,...
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2021-02-01
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| Series: | Bulletin of the Polish Academy of Sciences: Technical Sciences |
| Subjects: | |
| Online Access: | https://journals.pan.pl/Content/119101/PDF/30_01869_Bpast.No.69(1)_23.02.21_K1_A.pdf |
| _version_ | 1818503488877887488 |
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| author | Agnieszka Podwin Tymon Janisz Katarzyna Patejuk Piotr Szyszka Rafał Walczak Jan Dziuban |
| author_facet | Agnieszka Podwin Tymon Janisz Katarzyna Patejuk Piotr Szyszka Rafał Walczak Jan Dziuban |
| author_sort | Agnieszka Podwin |
| collection | DOAJ |
| description | This paper presents material and technological studies on lab-on-chip (LOC) devices as a first step towards biocompatible and reliable research on microscopic fungi and soil organisms on a microscale. This approach is intended to respond to the growing need for environmental control and protection, by means of modern, miniaturized, portable and dependable microfluidics instrumentation. The authors have presented herein long-term, successful cultivation of different fungi representatives (with emphasis put on Cladosporium macrocarpum) in specially fabricated all-glass LOCs. Notable differences were noted in the development of these creatures on polymer, polydimethylosiloxane (PDMS) cultivation substrates, revealing the uncommon morphological character of the fungi mycelium. The utility of all-glass LOCs was verified for other fungi representatives as well – Fusarium culmorum and Pencilium expansum, showing technical correspondence and biocompatibility of the devices. On that basis, other future applications of the solution are possible, covering, e.g. investigation of additional, environmentally relevant fungi species. Further development of the LOC instrumentation is also taken into consideration, which could be used for cultivation of other soil organisms and study of their mutual relationships within the integrated microfluidic device. |
| first_indexed | 2024-12-10T21:24:39Z |
| format | Article |
| id | doaj.art-2c9e18e1735a40e49c4126e36dd6b518 |
| institution | Directory Open Access Journal |
| issn | 2300-1917 |
| language | English |
| last_indexed | 2024-12-10T21:24:39Z |
| publishDate | 2021-02-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Bulletin of the Polish Academy of Sciences: Technical Sciences |
| spelling | doaj.art-2c9e18e1735a40e49c4126e36dd6b5182022-12-22T01:33:01ZengPolish Academy of SciencesBulletin of the Polish Academy of Sciences: Technical Sciences2300-19172021-02-0169No. 1https://doi.org/10.24425/bpasts.2020.136212Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organismsAgnieszka Podwin0Tymon Janisz1Katarzyna Patejuk2Piotr Szyszka3Rafał Walczak4Jan Dziuban5Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, ul. Janiszewskiego 11/17, 50-372 Wrocław, PolandWrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, ul. Janiszewskiego 11/17, 50-372 Wrocław, PolandWrocław University of Environmental and Life Sciences, Department of Plant Protection, Grunwaldzki Sq. 24a, 50-363 Wroclaw, PolandWrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, ul. Janiszewskiego 11/17, 50-372 Wrocław, PolandWrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, ul. Janiszewskiego 11/17, 50-372 Wrocław, PolandWrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, ul. Janiszewskiego 11/17, 50-372 Wrocław, PolandThis paper presents material and technological studies on lab-on-chip (LOC) devices as a first step towards biocompatible and reliable research on microscopic fungi and soil organisms on a microscale. This approach is intended to respond to the growing need for environmental control and protection, by means of modern, miniaturized, portable and dependable microfluidics instrumentation. The authors have presented herein long-term, successful cultivation of different fungi representatives (with emphasis put on Cladosporium macrocarpum) in specially fabricated all-glass LOCs. Notable differences were noted in the development of these creatures on polymer, polydimethylosiloxane (PDMS) cultivation substrates, revealing the uncommon morphological character of the fungi mycelium. The utility of all-glass LOCs was verified for other fungi representatives as well – Fusarium culmorum and Pencilium expansum, showing technical correspondence and biocompatibility of the devices. On that basis, other future applications of the solution are possible, covering, e.g. investigation of additional, environmentally relevant fungi species. Further development of the LOC instrumentation is also taken into consideration, which could be used for cultivation of other soil organisms and study of their mutual relationships within the integrated microfluidic device.https://journals.pan.pl/Content/119101/PDF/30_01869_Bpast.No.69(1)_23.02.21_K1_A.pdfmicrofluidicslab-chipbiomaterialscell cultivationmycology |
| spellingShingle | Agnieszka Podwin Tymon Janisz Katarzyna Patejuk Piotr Szyszka Rafał Walczak Jan Dziuban Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organisms Bulletin of the Polish Academy of Sciences: Technical Sciences microfluidics lab-chip biomaterials cell cultivation mycology |
| title | Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organisms |
| title_full | Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organisms |
| title_fullStr | Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organisms |
| title_full_unstemmed | Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organisms |
| title_short | Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organisms |
| title_sort | towards microfluidics for mycology material and technological studies on locs as new tools ensuring investigation of microscopic fungi and soil organisms |
| topic | microfluidics lab-chip biomaterials cell cultivation mycology |
| url | https://journals.pan.pl/Content/119101/PDF/30_01869_Bpast.No.69(1)_23.02.21_K1_A.pdf |
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